Rabbit Recombinant Monoclonal GOLPH4/GPP130 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Images
![Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] - BSA and Azide free (AB251244), expandable thumbnail](https://content.abcam.com/products/images/golph4-gpp130-antibody-epr13439-bsa-and-azide-free-ab251244--western-blot-img271.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] - BSA and Azide free (AB251244)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
8 products for Alternative Version
Target data
Function
Plays a role in endosome to Golgi protein trafficking; mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi.
Alternative names
Golgi integral membrane protein 4, Golgi phosphoprotein 4, Golgi-localized phosphoprotein of 130 kDa, GIMPc, Golgi phosphoprotein of 130 kDa, GOLIM4, GIMPC, GOLPH4, GPP130
Recommended products
Rabbit Recombinant Monoclonal GOLPH4/GPP130 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Alternative names
Golgi integral membrane protein 4, Golgi phosphoprotein 4, Golgi-localized phosphoprotein of 130 kDa, GIMPc, Golgi phosphoprotein of 130 kDa, GOLIM4, GIMPC, GOLPH4, GPP130
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR13439
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab251244 is the carrier-free version of Anti-GOLPH4/GPP130 antibody [EPR13439] ab197595.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
GOLPH4 also known as GPP130 and Golgi integral membrane protein 4 is a protein with a mass of approximately 130 kDa. It plays an important role in the organization and maintenance of the Golgi apparatus structure. GOLPH4 is predominantly found in the cis-Golgi network and it is involved in the cellular sorting and trafficking of proteins and lipids. The protein functions as a tethering factor and it may participate in the transport of cargo from the Golgi to other cellular locations.
- Biological function summary
The role of GOLPH4 centers around its involvement in the protein trafficking process within cells. It acts as part of the complex tasked with maintaining Golgi organization which is important for facilitating accurate cargo sorting and delivery. GOLPH4's location and structure enable it to interact with motor proteins and other Golgi matrix proteins aiding in vesicle tethering and fusion processes.
- Pathways
GOLPH4 is involved in essential cellular pathways such as the secretion and endocytic trafficking pathways. It interacts with other Golgi-resident proteins like GM130 and giantin which are essential for the movement of materials through the Golgi and to their final destinations. These pathways ensure that proteins and lipids reach their intended areas within the cell or are secreted to the extracellular space which is important for maintaining cellular function and homeostasis.
- Associated diseases and disorders
Disturbances in GOLPH4 function have been associated with conditions such as cancer and neurodegenerative diseases. Aberrant expression or mutations in this protein can lead to misregulation of Golgi functions affecting overall cellular trafficking systems. GOLPH4's interaction with proteins involved in these conditions such as p53 in cancer or tau in neurodegenerative diseases highlights its potential as a target for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] - BSA and Azide free (ab251244), expandable thumbnail](https://content.abcam.com/products/images/golph4-gpp130-antibody-epr13439-bsa-and-azide-free-ab251244--western-blot-img271.jpg "Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] - BSA and Azide free (ab251244)")
Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] - BSA and Azide free (ab251244)
This data was developed using the same antibody clone in a different buffer formulation (Anti-GOLPH4/GPP130 antibody [EPR13439] ab197595).
Lanes 1-4: Merged signal (red and green). Green - Anti-GOLPH4/GPP130 antibody [EPR13439] ab197595 observed at 130 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
Anti-GOLPH4/GPP130 antibody [EPR13439] ab197595 Anti-GOLPH4/GPP130 antibody [EPR13439] was shown to specifically react with GOLPH4/GPP130 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell line ab266123 (knockout cell lysate Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell lysate ab257455) was used. Wild-type and GOLPH4/GPP130 knockout samples were subjected to SDS-PAGE. Anti-GOLPH4/GPP130 antibody [EPR13439] ab197595 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] (Anti-GOLPH4/GPP130 antibody [EPR13439] ab197595) at 1/1000 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: GOLIM4 knockout HEK293T cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: MOLT-4 cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 82 kDa
Observed band size: 130 kDa
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