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AB204335

Anti-GORASP2/GRASP55 antibody

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(3 Publications)

Rabbit Polyclonal GORASP2/GRASP55 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GORASP2 aa 350 to C-terminus.

View Alternative Names

GOLPH6, GORASP2, Golgi reassembly-stacking protein 2, GRS2, Golgi phosphoprotein 6, Golgi reassembly-stacking protein of 55 kDa, p59, GRASP55

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GORASP2/GRASP55 antibody (AB204335)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GORASP2/GRASP55 antibody (AB204335)

Immunohistochemical analysis of Paraffin-embedded Human duodenum tissue labeling GORASP2 with ab204335 at 1/50 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-GORASP2/GRASP55 antibody (AB204335)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GORASP2/GRASP55 antibody (AB204335)

Immunofluorescent analysis of A431 cells (PFA-fixed/Triton X-100 permeabilized) labeling GORASP2 with ab204335 at 4 μg/ml (green).

Western blot - Anti-GORASP2/GRASP55 antibody (AB204335)
  • WB

Supplier Data

Western blot - Anti-GORASP2/GRASP55 antibody (AB204335)

All lanes:

Western blot - Anti-GORASP2/GRASP55 antibody (ab204335) at 1/100 dilution

Lane 1:

RT4 cell lysate

Lane 2:

U251 MG cell lysate

Lane 3:

Human plasma lysate

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GORASP2 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H8Y8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GORASP2 also known as GRASP55 is a golgi reassembly stacking protein with a mass approximately 55 kDa. It is highly expressed in the Golgi apparatus where it plays an important role in maintaining the structure and function of this organelle. GRASP55 facilitates the stacking of Golgi cisternae which is essential for Golgi apparatus organization. The protein acts alongside its isoform GRASP65 though they function somewhat independently in different Golgi regions.
Biological function summary

GRASP55 controls various cellular processes including the trafficking of proteins and lipids within the cell. It is part of the GRASP complex along with GRASP65 facilitating membrane tethering and fusion events. The protein's function is also linked to its ability to regulate Golgi's response to cellular stress or changes in environment impacting secretion and growth factor availability. In some conditions GRASP55 can influence processes such as autophagy underlining its diverse biological actions within the cell.

Pathways

GRASP55 integrates into significant cellular pathways like the secretory pathway and the autophagy pathway. It interacts with key proteins like golgins and Rab GTPases to assist in vesicle tethering and transport. GRASP55 also closely interacts with p62 a protein that plays a critical role in the selective autophagy pathway. This positions GRASP55 as a contributor to not only the routine processing of cellular materials but also cellular adaptation to stress through autophagy modulation.

GRASP55 shows relevance to cancer progression and neurodegenerative diseases. Aberrant expression or dysfunction of GRASP55 can contribute to cancerous transformations by altering cell adhesion and signaling pathways. Its role in autophagy connects it to diseases like Alzheimer's where defective protein processing is a hallmark. The interaction of GRASP55 with proteins such as p62 becomes particularly important as disrupted autophagy pathways are often implicated in neurodegenerative conditions highlighting GRASP55's significant role in both health and disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key structural protein of the Golgi apparatus (PubMed : 33301566). The membrane cisternae of the Golgi apparatus adhere to each other to form stacks, which are aligned side by side to form the Golgi ribbon (PubMed : 33301566). Acting in concert with GORASP1/GRASP65, is required for the formation and maintenance of the Golgi ribbon, and may be dispensable for the formation of stacks (PubMed : 33301566). However, other studies suggest that GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after breakdown during mitosis and meiosis (PubMed : 10487747, PubMed : 21515684, PubMed : 22523075). May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA (PubMed : 11101516). Required for normal acrosome formation during spermiogenesis and normal male fertility, probably by promoting colocalization of JAM2 and JAM3 at contact sites between germ cells and Sertoli cells (By similarity). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (PubMed : 21884936, PubMed : 27062250, PubMed : 28067262).
See full target information GORASP2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Clinical science (London, England : 1979) 138:1131-1150 PubMed39282930

2024

Identifying mitigating strategies for endothelial cell dysfunction and hypertension in response to VEGF receptor inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas D Camarda,Qing Lu,Dawn M Meola,Joshua J Man,Zeyuan Song,Richard J Travers,Katherine E Lopez,Sarah N Powers,Malvina Papanastasiou,Katherine C DeRuff,James Mullahoo,Shawn B Egri,Desiree Davison,Paola Sebastiani,Scott T Eblen,Rachel Buchsbaum,Gordon S Huggins,Cheryl A London,Jacob D Jaffe,Jenica N Upshaw,Vicky K Yang,Iris Z Jaffe

International journal of molecular sciences 24: PubMed37373263

2023

Golgi Reassembly Stacking Protein 2 Modulates Myometrial Contractility during Labor by Affecting ATP Production.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yang,Lina Chen,Bolun Wen,Xiaodi Wang,Lele Wang,Kaiyuan Ji,Huishu Liu

Nature communications 12:2951 PubMed34012031

2021

FKRP-dependent glycosylation of fibronectin regulates muscle pathology in muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

A J Wood,C H Lin,M Li,K Nishtala,S Alaei,F Rossello,C Sonntag,L Hersey,L B Miles,C Krisp,S Dudczig,A J Fulcher,S Gibertini,P J Conroy,A Siegel,M Mora,P Jusuf,N H Packer,P D Currie
View all publications
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Product promise

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