AB211532

Anti-GORASP2/GRASP55 antibody [CL2610]

Name: Anti-GORASP2/GRASP55 antibody [CL2610] (ab211532) | Abcam
Brand: Abcam Limited
SKU: AB211532
Price: 495 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(5 Publications)

Mouse Monoclonal GORASP2/GRASP55 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GORASP2 aa 350-500.

View Alternative Names

GOLPH6, GORASP2, Golgi reassembly-stacking protein 2, GRS2, Golgi phosphoprotein 6, Golgi reassembly-stacking protein of 55 kDa, p59, GRASP55

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human epidiymis tissue labelling GORASP2/GRASP55 with ab211532 at 1/5000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunocytochemistry - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

Immunofluorescent analysis of A431 cells (PFA-fixed/Triton X-100 permeabilized) labeling GORASP2 with ab211532 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling GORASP2/GRASP55 with ab211532 at 1/5000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunocytochemistry - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

Immunofluorescent analysis of U251 cells (PFA-fixed/Triton X-100 permeabilized) labeling GORASP2 with ab211532 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.

Supplier Data

Immunocytochemistry - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

Immunofluorescent analysis of U2OS cells (PFA-fixed/Triton X-100 permeabilized) labeling GORASP2 with ab211532 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.

Supplier Data

Immunocytochemistry - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

Immunofluorescent analysis of HeLa cells (PFA-fixed/Triton X-100 permeabilized) labeling GORASP2 with ab211532 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.

Supplier Data

Immunocytochemistry - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

Immunofluorescent analysis of MCF7 cells (PFA-fixed/Triton X-100 permeabilized) labeling GORASP2 with ab211532 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling GORASP2/GRASP55 with ab211532 at 1/5000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling GORASP2/GRASP55 with ab211532 at 1/5000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.

All lanes:

Western blot - Anti-GORASP2/GRASP55 antibody [CL2610] (ab211532)

Lane 1:

U-251 cells, transfected with control siRNA

Lane 2:

U-251 cells, transfected with target specific siRNA probe #1

Lane 3:

U-251 cells, transfected with target specific siRNA probe #2

Predicted band size: 47 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL2610

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC

applications

Immunogen

Recombinant Fragment Protein within Human GORASP2 aa 350-500. The exact immunogen used to generate this antibody is proprietary information.

Q9H8Y8

Epitope

Binds to an epitope located within the peptide sequence DAASSLTVDVTPPTA (aa 405-412) as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/10000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2-10 µg/mL", "ICC-species-notes": "Fixation/Permeabilization: PFA/Triton X-100", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GORASP2 also known as GRASP55 is a golgi reassembly stacking protein with a mass approximately 55 kDa. It is highly expressed in the Golgi apparatus where it plays an important role in maintaining the structure and function of this organelle. GRASP55 facilitates the stacking of Golgi cisternae which is essential for Golgi apparatus organization. The protein acts alongside its isoform GRASP65 though they function somewhat independently in different Golgi regions.

Biological function summary

GRASP55 controls various cellular processes including the trafficking of proteins and lipids within the cell. It is part of the GRASP complex along with GRASP65 facilitating membrane tethering and fusion events. The protein's function is also linked to its ability to regulate Golgi's response to cellular stress or changes in environment impacting secretion and growth factor availability. In some conditions GRASP55 can influence processes such as autophagy underlining its diverse biological actions within the cell.

Pathways

GRASP55 integrates into significant cellular pathways like the secretory pathway and the autophagy pathway. It interacts with key proteins like golgins and Rab GTPases to assist in vesicle tethering and transport. GRASP55 also closely interacts with p62 a protein that plays a critical role in the selective autophagy pathway. This positions GRASP55 as a contributor to not only the routine processing of cellular materials but also cellular adaptation to stress through autophagy modulation.

GRASP55 shows relevance to cancer progression and neurodegenerative diseases. Aberrant expression or dysfunction of GRASP55 can contribute to cancerous transformations by altering cell adhesion and signaling pathways. Its role in autophagy connects it to diseases like Alzheimer's where defective protein processing is a hallmark. The interaction of GRASP55 with proteins such as p62 becomes particularly important as disrupted autophagy pathways are often implicated in neurodegenerative conditions highlighting GRASP55's significant role in both health and disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key structural protein of the Golgi apparatus (PubMed : 33301566). The membrane cisternae of the Golgi apparatus adhere to each other to form stacks, which are aligned side by side to form the Golgi ribbon (PubMed : 33301566). Acting in concert with GORASP1/GRASP65, is required for the formation and maintenance of the Golgi ribbon, and may be dispensable for the formation of stacks (PubMed : 33301566). However, other studies suggest that GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after breakdown during mitosis and meiosis (PubMed : 10487747, PubMed : 21515684, PubMed : 22523075). May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA (PubMed : 11101516). Required for normal acrosome formation during spermiogenesis and normal male fertility, probably by promoting colocalization of JAM2 and JAM3 at contact sites between germ cells and Sertoli cells (By similarity). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (PubMed : 21884936, PubMed : 27062250, PubMed : 28067262).

See full target information GORASP2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:532 PubMed38710927

2024

SUMOylated Golgin45 associates with PML-NB to transcriptionally regulate lipid metabolism genes during heat shock stress.

Applications

Unspecified application

Species

Unspecified reactive species

Shuaiyang Jing,Jingkai Gao,Neeraj Tiwari,Yulei Du,Lianhui Zhu,Bopil Gim,Yi Qian,Xihua Yue,Intaek Lee

The Journal of cell biology 221: PubMed36099524

2022

Impaired XK recycling for importing manganese underlies striatal vulnerability in Huntington's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Gaurav Chhetri,Yuting Ke,Ping Wang,Muhammad Usman,Yan Li,Ellen Sapp,Jing Wang,Arabinda Ghosh,Md Ariful Islam,Xiaolong Wang,Adel Boudi,Marian DiFiglia,Xueyi Li

Communications biology 4:1370 PubMed34876695

2021

Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xihua Yue,Neeraj Tiwari,Lianhui Zhu,Hai Dang Truong Ngo,Jae-Min Lim,Bopil Gim,Shuaiyang Jing,Yijing Wang,Yi Qian,Intaek Lee

Biology open 10: PubMed34533192

2021

GRASP55 restricts early-stage autophagy and regulates spatial organization of the early secretory network.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Y Liu,Yu-Hsiu Tony Lin,Andrew M Leidal,Hector H Huang,Jordan Ye,Arun P Wiita,Jayanta Debnath

International journal of molecular sciences 22: PubMed34502507

2021

Regulation of the Golgi Apparatus by p38 and JNK Kinases during Cellular Stress Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Cathrine Nordgaard,Maxim A X Tollenaere,Ana Martinez Del Val,Dorte B Bekker-Jensen,Melanie Blasius,Jesper V Olsen,Simon Bekker-Jensen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GORASP2/GRASP55 antibody [CL2610]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

Immunocytochemistry - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

Immunocytochemistry - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

Immunocytochemistry - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

Immunocytochemistry - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

Immunocytochemistry - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

Western blot - Anti-GORASP2/GRASP55 antibody [CL2610] (AB211532)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise