AB88462

Anti-GOSR1/GS28 antibody

Name: Anti-GOSR1/GS28 antibody (ab88462) | Abcam
Brand: Abcam Limited
SKU: AB88462
Price: 545 USD
Availability: InStock

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(2 Publications)

Mouse Polyclonal GOSR1/GS28 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GOSR1.

View Alternative Names

GS28, GOSR1, Golgi SNAP receptor complex member 1, 28 kDa Golgi SNARE protein, 28 kDa cis-Golgi SNARE p28, GOS-28

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody (AB88462)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody (AB88462)

Immunofluorescence of ab88462 (used at 10μg/ml) to GOSR1/GS28 on HeLa cells.

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Western blot - Anti-GOSR1/GS28 antibody (AB88462)

All lanes:

Western blot - Anti-GOSR1/GS28 antibody (ab88462) at 5 µg/mL

All lanes:

Human liver lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG at 1/2500 dilution

Predicted band size: 29 kDa

Observed band size: 25 kDa

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Western blot - Anti-GOSR1/GS28 antibody (AB88462)

All lanes:

Western blot - Anti-GOSR1/GS28 antibody (ab88462) at 5 µg/mL

Lane 1:

Transfected 293T cell line at 25 µg

Lane 2:

Non-transfected cell line at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG at 1/2500 dilution

Predicted band size: 29 kDa

Observed band size: 27 kDa

false

Unknown

Western blot - Anti-GOSR1/GS28 antibody (AB88462)

All lanes:

Western blot - Anti-GOSR1/GS28 antibody (ab88462) at 5 µg/mL

All lanes:

A-431 cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG at 1/2500 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GOSR1.

O95249

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GOSR1 also known as GS28 is a Golgi SNAP receptor complex member and has a mass of approximately 28 kDa. It functions mechanically in the membrane trafficking process facilitating the fusion of transport vesicles with the Golgi apparatus. GOSR1 localizes mainly to the Golgi apparatus and is expressed in numerous human tissues including the liver brain and pancreas. It participates in the assembly of the SNARE complex which is important for vesicle docking and fusion.

Biological function summary

GOSR1 is involved in the intracellular transport system ensuring the proper sorting and distribution of proteins within cells. It is a component of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex working with other SNARE proteins to mediate vesicle fusion events. This interaction plays a significant role in maintaining the functionality and organization of the Golgi network impacting protein processing and trafficking.

Pathways

GOSR1 plays a role in intracellular transport and protein processing pathways influencing how proteins are modified and directed within the cell. It interacts with other proteins such as syntaxin 5 and Bet1 to facilitate the fusion of vesicles with the Golgi. This action integrates GOSR1 into the Golgi-to-endoplasmic reticulum retrograde transport pathway as well as anterograde transport which are essential for the maintenance of cellular protein homeostasis.

Alterations in GOSR1 expression or function may relate to neurodegenerative conditions such as Parkinson's disease and metabolic disorders like diabetes. GOSR1's role in vesicle trafficking means its dysfunction can disrupt cellular homeostasis potentially leading to these diseases. It interacts with proteins such as alpha-synuclein in neurodegeneration and may influence insulin granule trafficking in metabolic disorders. Understanding GOSR1's involvement opens potential avenues for therapeutic approaches targeting these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor. May play a protective role against hydrogen peroxide induced cytotoxicity under glutathione depleted conditions in neuronal cells by regulating the intracellular ROS levels via inhibition of p38 MAPK (MAPK11, MAPK12, MAPK13 and MAPK14). Participates in docking and fusion stage of ER to cis-Golgi transport. Plays an important physiological role in VLDL-transport vesicle-Golgi fusion and thus in VLDL delivery to the hepatic cis-Golgi.

See full target information GOSR1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Methods in molecular biology (Clifton, N.J.) 2557:161-209 PubMed36512216

2022

Algorithm for Modern Electron Microscopic Examination of the Golgi Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander A Mironov,Galina V Beznoussenko

Nature medicine 17:610-7 PubMed21499269

2011

B cells promote insulin resistance through modulation of T cells and production of pathogenic IgG antibodies.

Applications

Species

Human

Daniel A Winer,Shawn Winer,Lei Shen,Persis P Wadia,Jason Yantha,Geoffrey Paltser,Hubert Tsui,Ping Wu,Matthew G Davidson,Michael N Alonso,Hwei X Leong,Alec Glassford,Maria Caimol,Justin A Kenkel,Thomas F Tedder,Tracey McLaughlin,David B Miklos,H-Michael Dosch,Edgar G Engleman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GOSR1/GS28 antibody

Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody (AB88462)

Western blot - Anti-GOSR1/GS28 antibody (AB88462)

Western blot - Anti-GOSR1/GS28 antibody (AB88462)

Western blot - Anti-GOSR1/GS28 antibody (AB88462)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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