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Rabbit Recombinant Monoclonal GOSR1/GS28 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Not recommended
Tested
Tested
Tested
Tested
Mouse
Not recommended
Tested
Tested
Expected
Expected
Rat
Not recommended
Tested
Expected
Expected
Expected

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

-

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

For unpurified use at 1/250 - 1/500.

Species

Rat

Dilution info

1/1000

Notes

For unpurified use at 1/250 - 1/500.

Species

Human

Dilution info

1/1000

Notes

For unpurified use at 1/250 - 1/500.

Tested
Tested

Species

Mouse

Dilution info

1/150

Notes

For unpurified use at 1/50 - 1/100.

Species

Human

Dilution info

1/150

Notes

For unpurified use at 1/50 - 1/100.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/120

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/30.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor. May play a protective role against hydrogen peroxide induced cytotoxicity under glutathione depleted conditions in neuronal cells by regulating the intracellular ROS levels via inhibition of p38 MAPK (MAPK11, MAPK12, MAPK13 and MAPK14). Participates in docking and fusion stage of ER to cis-Golgi transport. Plays an important physiological role in VLDL-transport vesicle-Golgi fusion and thus in VLDL delivery to the hepatic cis-Golgi.

Alternative names

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Rabbit Recombinant Monoclonal GOSR1/GS28 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP1768Y

Purification technique

Affinity purification Protein A

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

GOSR1 is involved in the intracellular transport system ensuring the proper sorting and distribution of proteins within cells. It is a component of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex working with other SNARE proteins to mediate vesicle fusion events. This interaction plays a significant role in maintaining the functionality and organization of the Golgi network impacting protein processing and trafficking.

Activity summary

GOSR1 also known as GS28 is a Golgi SNAP receptor complex member and has a mass of approximately 28 kDa. It functions mechanically in the membrane trafficking process facilitating the fusion of transport vesicles with the Golgi apparatus. GOSR1 localizes mainly to the Golgi apparatus and is expressed in numerous human tissues including the liver brain and pancreas. It participates in the assembly of the SNARE complex which is important for vesicle docking and fusion.

Pathways

GOSR1 plays a role in intracellular transport and protein processing pathways influencing how proteins are modified and directed within the cell. It interacts with other proteins such as syntaxin 5 and Bet1 to facilitate the fusion of vesicles with the Golgi. This action integrates GOSR1 into the Golgi-to-endoplasmic reticulum retrograde transport pathway as well as anterograde transport which are essential for the maintenance of cellular protein homeostasis.

Associated diseases and disorders

Alterations in GOSR1 expression or function may relate to neurodegenerative conditions such as Parkinson's disease and metabolic disorders like diabetes. GOSR1's role in vesicle trafficking means its dysfunction can disrupt cellular homeostasis potentially leading to these diseases. It interacts with proteins such as alpha-synuclein in neurodegeneration and may influence insulin granule trafficking in metabolic disorders. Understanding GOSR1's involvement opens potential avenues for therapeutic approaches targeting these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Flow Cytometry (Intracellular) - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GOSR1/GS28 with purified ab53288 at 1/120 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288)

    Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling GOSR1/GS28 with Purified ab53288 at 1:150 dilution. Cells were fixed in 100% Methanol. Cells were counterstained with None. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288), expandable thumbnail

    Western blot - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288)

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-GOSR1/GS28 antibody [EP1768Y] (AB53288) at 1/1000 dilution

    Lane 1: SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

    Lane 3: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates at 20 µg

    Lane 4: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 20 µg

    Lane 5: C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

    Lane 6: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 20 µg

    Lane 7: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 29 kDa

    Observed band size: 29 kDa

  • Western blot - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288), expandable thumbnail

    Western blot - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288)

    All lanes: Western blot - Anti-GOSR1/GS28 antibody [EP1768Y] (AB53288) at 1/500 dilution

    Lane 1: SKBR-3 cell lysate at 10 µg

    Lane 2: 3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 29 kDa

    Observed band size: 29 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288)

    HeLa cells stained with unpurified ab53288 at 1/50 - 1/100 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling GOSR1/GS28 with Purified ab53288 at 1:100 dilution (12.3 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288)

    Human stomach carcinoma staining with unpurified ab53288 at 1/100 - 1/250 dilution

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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