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AB53288

Anti-GOSR1/GS28 antibody [EP1768Y]

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(1 Review)

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(3 Publications)

Rabbit Recombinant Monoclonal GOSR1/GS28 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

View Alternative Names

GS28, GOSR1, Golgi SNAP receptor complex member 1, 28 kDa Golgi SNARE protein, 28 kDa cis-Golgi SNARE p28, GOS-28

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] (AB53288)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] (AB53288)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling GOSR1/GS28 with Purified ab53288 at 1 : 100 dilution (12.3 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

Flow Cytometry (Intracellular) - Anti-GOSR1/GS28 antibody [EP1768Y] (AB53288)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-GOSR1/GS28 antibody [EP1768Y] (AB53288)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GOSR1/GS28 with purified ab53288 at 1/120 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] (AB53288)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] (AB53288)

Human stomach carcinoma staining with unpurified ab53288 at 1/100 - 1/250 dilution

Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] (AB53288)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] (AB53288)

HeLa cells stained with unpurified ab53288 at 1/50 - 1/100 dilution

Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] (AB53288)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] (AB53288)

Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling GOSR1/GS28 with Purified ab53288 at 1 : 150 dilution. Cells were fixed in 100% Methanol. Cells were counterstained with None. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Western blot - Anti-GOSR1/GS28 antibody [EP1768Y] (AB53288)
  • WB

Lab

Western blot - Anti-GOSR1/GS28 antibody [EP1768Y] (AB53288)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288) at 1/1000 dilution

Lane 1:

SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

Lane 3:

Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates at 20 µg

Lane 4:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 20 µg

Lane 5:

C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

Lane 6:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 20 µg

Lane 7:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Western blot - Anti-GOSR1/GS28 antibody [EP1768Y] (AB53288)
  • WB

Unknown

Western blot - Anti-GOSR1/GS28 antibody [EP1768Y] (AB53288)

All lanes:

Western blot - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288) at 1/500 dilution

Lane 1:

SKBR-3 cell lysate at 10 µg

Lane 2:

3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

  • Carrier free

    Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1768Y

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/250 - 1/500. </strong></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/150", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/50 - 1/100. </strong></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/120", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/30.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.</p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/250 - 1/500. </strong></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/150", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/50 - 1/100. </strong></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/250 - 1/500. </strong></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GOSR1 also known as GS28 is a Golgi SNAP receptor complex member and has a mass of approximately 28 kDa. It functions mechanically in the membrane trafficking process facilitating the fusion of transport vesicles with the Golgi apparatus. GOSR1 localizes mainly to the Golgi apparatus and is expressed in numerous human tissues including the liver brain and pancreas. It participates in the assembly of the SNARE complex which is important for vesicle docking and fusion.
Biological function summary

GOSR1 is involved in the intracellular transport system ensuring the proper sorting and distribution of proteins within cells. It is a component of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex working with other SNARE proteins to mediate vesicle fusion events. This interaction plays a significant role in maintaining the functionality and organization of the Golgi network impacting protein processing and trafficking.

Pathways

GOSR1 plays a role in intracellular transport and protein processing pathways influencing how proteins are modified and directed within the cell. It interacts with other proteins such as syntaxin 5 and Bet1 to facilitate the fusion of vesicles with the Golgi. This action integrates GOSR1 into the Golgi-to-endoplasmic reticulum retrograde transport pathway as well as anterograde transport which are essential for the maintenance of cellular protein homeostasis.

Alterations in GOSR1 expression or function may relate to neurodegenerative conditions such as Parkinson's disease and metabolic disorders like diabetes. GOSR1's role in vesicle trafficking means its dysfunction can disrupt cellular homeostasis potentially leading to these diseases. It interacts with proteins such as alpha-synuclein in neurodegeneration and may influence insulin granule trafficking in metabolic disorders. Understanding GOSR1's involvement opens potential avenues for therapeutic approaches targeting these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor. May play a protective role against hydrogen peroxide induced cytotoxicity under glutathione depleted conditions in neuronal cells by regulating the intracellular ROS levels via inhibition of p38 MAPK (MAPK11, MAPK12, MAPK13 and MAPK14). Participates in docking and fusion stage of ER to cis-Golgi transport. Plays an important physiological role in VLDL-transport vesicle-Golgi fusion and thus in VLDL delivery to the hepatic cis-Golgi.
See full target information GOSR1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 8: PubMed37192004

2023

JP1 normalizes tumor vasculature to suppress metastasis and facilitate drug delivery by inhibiting IL-8.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahua Cui,Zhen Che,Lu Zou,Dongyin Chen,Zhan Xie,Kun Ding,Huning Jiang,Aiping Li,Jianwei Zhou,Yongqian Shu

Nature communications 13:4918 PubMed35995799

2022

Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Xue Xie,Nima N Naseri,Jasmine Fels,Parinati Kharel,Yoonmi Na,Diane Lane,Jacqueline Burré,Manu Sharma

The Journal of biological chemistry 293:17505-17522 PubMed30242129

2018

Complex formation of sphingomyelin synthase 1 with glucosylceramide synthase increases sphingomyelin and decreases glucosylceramide levels.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuhiro Hayashi,Yoko Nemoto-Sasaki,Naoki Matsumoto,Kotaro Hama,Takashi Tanikawa,Saori Oka,Tadaaki Saeki,Tatsuya Kumasaka,Takanori Koizumi,Seisuke Arai,Ikuo Wada,Kazuaki Yokoyama,Takayuki Sugiura,Atsushi Yamashita
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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