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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Monoclonal GOSR1/GS28 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
---|---|---|---|---|---|
Human | Not recommended | Tested | Tested | Tested | Tested |
Mouse | Not recommended | Tested | Tested | Expected | Expected |
Rat | Not recommended | Tested | Expected | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes For unpurified use at 1/250 - 1/500. |
Species Rat | Dilution info 1/1000 | Notes For unpurified use at 1/250 - 1/500. |
Species Human | Dilution info 1/1000 | Notes For unpurified use at 1/250 - 1/500. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/150 | Notes For unpurified use at 1/50 - 1/100. |
Species Human | Dilution info 1/150 | Notes For unpurified use at 1/50 - 1/100. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/120 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/30. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor. May play a protective role against hydrogen peroxide induced cytotoxicity under glutathione depleted conditions in neuronal cells by regulating the intracellular ROS levels via inhibition of p38 MAPK (MAPK11, MAPK12, MAPK13 and MAPK14). Participates in docking and fusion stage of ER to cis-Golgi transport. Plays an important physiological role in VLDL-transport vesicle-Golgi fusion and thus in VLDL delivery to the hepatic cis-Golgi.
Golgi SNAP receptor complex member 1, 28 kDa Golgi SNARE protein, 28 kDa cis-Golgi SNARE p28, GOS-28, GOSR1, GS28
Rabbit Recombinant Monoclonal GOSR1/GS28 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Golgi SNAP receptor complex member 1, 28 kDa Golgi SNARE protein, 28 kDa cis-Golgi SNARE p28, GOS-28, GOSR1, GS28
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EP1768Y
Affinity purification Protein A
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Stable for 12 months at -20°C
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
GOSR1 is involved in the intracellular transport system ensuring the proper sorting and distribution of proteins within cells. It is a component of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex working with other SNARE proteins to mediate vesicle fusion events. This interaction plays a significant role in maintaining the functionality and organization of the Golgi network impacting protein processing and trafficking.
GOSR1 also known as GS28 is a Golgi SNAP receptor complex member and has a mass of approximately 28 kDa. It functions mechanically in the membrane trafficking process facilitating the fusion of transport vesicles with the Golgi apparatus. GOSR1 localizes mainly to the Golgi apparatus and is expressed in numerous human tissues including the liver brain and pancreas. It participates in the assembly of the SNARE complex which is important for vesicle docking and fusion.
GOSR1 plays a role in intracellular transport and protein processing pathways influencing how proteins are modified and directed within the cell. It interacts with other proteins such as syntaxin 5 and Bet1 to facilitate the fusion of vesicles with the Golgi. This action integrates GOSR1 into the Golgi-to-endoplasmic reticulum retrograde transport pathway as well as anterograde transport which are essential for the maintenance of cellular protein homeostasis.
Alterations in GOSR1 expression or function may relate to neurodegenerative conditions such as Parkinson's disease and metabolic disorders like diabetes. GOSR1's role in vesicle trafficking means its dysfunction can disrupt cellular homeostasis potentially leading to these diseases. It interacts with proteins such as alpha-synuclein in neurodegeneration and may influence insulin granule trafficking in metabolic disorders. Understanding GOSR1's involvement opens potential avenues for therapeutic approaches targeting these conditions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GOSR1/GS28 with purified ab53288 at 1/120 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling GOSR1/GS28 with Purified ab53288 at 1:150 dilution. Cells were fixed in 100% Methanol. Cells were counterstained with None. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-GOSR1/GS28 antibody [EP1768Y] (AB53288) at 1/1000 dilution
Lane 1: SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 2: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg
Lane 3: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates at 20 µg
Lane 4: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 20 µg
Lane 5: C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg
Lane 6: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 20 µg
Lane 7: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
All lanes: Western blot - Anti-GOSR1/GS28 antibody [EP1768Y] (AB53288) at 1/500 dilution
Lane 1: SKBR-3 cell lysate at 10 µg
Lane 2: 3T3 cell lysate at 10 µg
All lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
HeLa cells stained with unpurified ab53288 at 1/50 - 1/100 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling GOSR1/GS28 with Purified ab53288 at 1:100 dilution (12.3 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Human stomach carcinoma staining with unpurified ab53288 at 1/100 - 1/250 dilution
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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