Rabbit Recombinant Monoclonal GOSR1/GS28 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (AB232339), expandable thumbnail](https://content.abcam.com/products/images/gosr1-gs28-antibody-ep1768y-bsa-and-azide-free-ab232339--flow-cytometry-intracellular-img26375.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (AB232339)")
![Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (AB232339), expandable thumbnail](https://content.abcam.com/products/images/gosr1-gs28-antibody-ep1768y-bsa-and-azide-free-ab232339--immunocytochemistry-immunofluorescence-img13896.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (AB232339)")
![Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (AB232339), expandable thumbnail](https://content.abcam.com/products/images/gosr1-gs28-antibody-ep1768y-bsa-and-azide-free-ab232339--immunocytochemistry-immunofluorescence-img135482.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (AB232339)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (AB232339), expandable thumbnail](https://content.abcam.com/products/images/gosr1-gs28-antibody-ep1768y-bsa-and-azide-free-ab232339--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107937.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (AB232339)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (AB232339), expandable thumbnail](https://content.abcam.com/products/images/gosr1-gs28-antibody-ep1768y-bsa-and-azide-free-ab232339--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img31034.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (AB232339)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Expected | Expected | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected |
| Rat | Expected | Predicted | Predicted | Predicted |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor. May play a protective role against hydrogen peroxide induced cytotoxicity under glutathione depleted conditions in neuronal cells by regulating the intracellular ROS levels via inhibition of p38 MAPK (MAPK11, MAPK12, MAPK13 and MAPK14). Participates in docking and fusion stage of ER to cis-Golgi transport. Plays an important physiological role in VLDL-transport vesicle-Golgi fusion and thus in VLDL delivery to the hepatic cis-Golgi.
Alternative names
Golgi SNAP receptor complex member 1, 28 kDa Golgi SNARE protein, 28 kDa cis-Golgi SNARE p28, GOS-28, GOSR1, GS28
Recommended products
Rabbit Recombinant Monoclonal GOSR1/GS28 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.
Alternative names
Golgi SNAP receptor complex member 1, 28 kDa Golgi SNARE protein, 28 kDa cis-Golgi SNARE p28, GOS-28, GOSR1, GS28
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EP1768Y
- Purification technique
Affinity purification Protein A
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab232339 is the carrier-free version of Anti-GOSR1/GS28 antibody [EP1768Y] ab53288.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GOSR1 also known as GS28 is a Golgi SNAP receptor complex member and has a mass of approximately 28 kDa. It functions mechanically in the membrane trafficking process facilitating the fusion of transport vesicles with the Golgi apparatus. GOSR1 localizes mainly to the Golgi apparatus and is expressed in numerous human tissues including the liver brain and pancreas. It participates in the assembly of the SNARE complex which is important for vesicle docking and fusion.
- Biological function summary
GOSR1 is involved in the intracellular transport system ensuring the proper sorting and distribution of proteins within cells. It is a component of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex working with other SNARE proteins to mediate vesicle fusion events. This interaction plays a significant role in maintaining the functionality and organization of the Golgi network impacting protein processing and trafficking.
- Pathways
GOSR1 plays a role in intracellular transport and protein processing pathways influencing how proteins are modified and directed within the cell. It interacts with other proteins such as syntaxin 5 and Bet1 to facilitate the fusion of vesicles with the Golgi. This action integrates GOSR1 into the Golgi-to-endoplasmic reticulum retrograde transport pathway as well as anterograde transport which are essential for the maintenance of cellular protein homeostasis.
- Associated diseases and disorders
Alterations in GOSR1 expression or function may relate to neurodegenerative conditions such as Parkinson's disease and metabolic disorders like diabetes. GOSR1's role in vesicle trafficking means its dysfunction can disrupt cellular homeostasis potentially leading to these diseases. It interacts with proteins such as alpha-synuclein in neurodegeneration and may influence insulin granule trafficking in metabolic disorders. Understanding GOSR1's involvement opens potential avenues for therapeutic approaches targeting these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry (Intracellular) - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339), expandable thumbnail](https://content.abcam.com/products/images/gosr1-gs28-antibody-ep1768y-bsa-and-azide-free-ab232339--flow-cytometry-intracellular-img26375.jpg "Flow Cytometry (Intracellular) - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339)")
Flow Cytometry (Intracellular) - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339)
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GOSR1/GS28 with purified Anti-GOSR1/GS28 antibody [EP1768Y] ab53288 at 1/120 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GOSR1/GS28 antibody [EP1768Y] ab53288).
![Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339), expandable thumbnail](https://content.abcam.com/products/images/gosr1-gs28-antibody-ep1768y-bsa-and-azide-free-ab232339--immunocytochemistry-immunofluorescence-img13896.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339)")
Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339)
Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling GOSR1/GS28 with Purified Anti-GOSR1/GS28 antibody [EP1768Y] ab53288 at 1:150 dilution. Cells were fixed in 100% Methanol. Cells were counterstained with None. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GOSR1/GS28 antibody [EP1768Y] ab53288).
![Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339), expandable thumbnail](https://content.abcam.com/products/images/gosr1-gs28-antibody-ep1768y-bsa-and-azide-free-ab232339--immunocytochemistry-immunofluorescence-img135482.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339)")
Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339)
HeLa cells stained with unpurified Anti-GOSR1/GS28 antibody [EP1768Y] ab53288 at 1/50 - 1/100 dilution
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GOSR1/GS28 antibody [EP1768Y] ab53288).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339), expandable thumbnail](https://content.abcam.com/products/images/gosr1-gs28-antibody-ep1768y-bsa-and-azide-free-ab232339--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107937.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339)
Human stomach carcinoma staining with unpurified Anti-GOSR1/GS28 antibody [EP1768Y] ab53288 at 1/100 - 1/250 dilution
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GOSR1/GS28 antibody [EP1768Y] ab53288).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339), expandable thumbnail](https://content.abcam.com/products/images/gosr1-gs28-antibody-ep1768y-bsa-and-azide-free-ab232339--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img31034.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling GOSR1/GS28 with Purified Anti-GOSR1/GS28 antibody [EP1768Y] ab53288 at 1:100 dilution (12.3 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GOSR1/GS28 antibody [EP1768Y] ab53288).
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