AB314107

Anti-GP130/CD130 antibody [BLR114H] - BSA free

Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal CD130 (gp130) antibody. Suitable for ICC, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL6ST aa 1-350.

View Alternative Names

CD130, Interleukin-6 receptor subunit beta, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL-6RB, CDw130, Interleukin-6 signal transducer, Membrane glycoprotein 130, Oncostatin-M receptor subunit alpha, gp130, IL6ST

4 Images

Supplier Data

Immunocytochemistry - Anti-GP130/CD130 antibody [BLR114H] - BSA free (AB314107)

Detection of human GP130/CD130 in FFPE HeLa cells by immunocytochemistry Antibody : Rabbit antiGP130/CD130 recombinant monoclonal antibody [BLR114H] (A700-114). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Supplier Data

Immunocytochemistry - Anti-GP130/CD130 antibody [BLR114H] - BSA free (AB314107)

Detection of human GP130/CD130 in FFPE GaMG cells by immunocytochemistry. Antibody : Rabbit antiGP130/CD130 recombinant monoclonal antibody [BLR114H] (A700-114). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Supplier Data

Immunocytochemistry - Anti-GP130/CD130 antibody [BLR114H] - BSA free (AB314107)

Detection of human GP130/CD130 in FFPE HUVEC-C cells by immunocytochemistry. Antibody : Rabbit antiGP130/CD130 recombinant monoclonal antibody [BLR114H] (A700-114). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Supplier Data

Western blot - Anti-GP130/CD130 antibody [BLR114H] - BSA free (AB314107)

Samples : Whole cell lysate (50 µg) from NCI-H226, MCF-7, IMR-90, Jurkat, HeLa, A-549, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody : Rabbit antiGP130/CD130 recombinant monoclonal antibody [BLR114H] (A700-114) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel : Rabbit anti-COPB2.

All lanes:

Western blot - Anti-GP130/CD130 antibody [BLR114H] - BSA free (ab314107) at 1/1000 dilution

Lane 1:

NCI-H226 Whole cell lysate at 50 µg

Lane 2:

MCF-7 Whole cell lysate at 50 µg

Lane 3:

IMR-90 Whole cell lysate at 50 µg

Lane 4:

Jurkat, Whole cell lysate at 50 µg

Lane 5:

HeLa Whole cell lysate at 50 µg

Lane 6:

A-549 Whole cell lysate at 50 µg

Lane 7:

Hep-G2 Whole cell lysate at 50 µg

Lane 8:

HEK293T Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR114H

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, WB

applications

Immunogen

Recombinant Fragment Protein within Human IL6ST aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

P40189

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

style

left-column

Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD130 also known as gp130 or the IL-6 signal transducer (IL6ST) is a protein weighing around 130 kDa. This receptor is encoded by the IL6ST gene and widely expressed on the surface of cells across multiple tissues. CD130 functions mechanically as a signal transducing receptor facilitating the assembly of receptor complexes necessary for the initiation of signal transduction. It plays a major role in cytokine receptor signaling particularly interacting with interleukin-6 (IL-6) family cytokines.

Biological function summary

CD130 functions as part of the high-affinity receptor complex for certain cytokines. It partners with other receptors such as CD126 to form a complex that initiates signaling pathways after ligand binding contributing to a range of immune responses. Its role is thorough across many cellular processes including differentiation proliferation and apoptosis. In addition to immune signaling CD130 is involved in several growth factor signaling pathways.

Pathways

CD130 serves a significant role in signal transduction particularly in the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway. It works in concert with related proteins like JAK1 and STAT3 to transmit signals from extracellular cytokines to the nucleus influencing gene expression. Additionally CD130 can also be found active in the MAPK/ERK pathway which is important for cellular response to growth signals.

CD130 plays a connection especially to inflammatory conditions and certain cancers. Aberrant signaling involving CD130 has been linked to disorders like rheumatoid arthritis where it contributes to the inflammation process. Additionally it relates to multiple myeloma a cancer where abnormal CD130 interactions with proteins like IL-6 may enhance cell survival and proliferation presenting potential targets for therapeutic intervention.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Signal-transducing molecule (PubMed : 2261637). The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates the intracellular JAK-MAPK and JAK-STAT3 signaling pathways (PubMed : 19915009, PubMed : 2261637, PubMed : 23294003). That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3 (PubMed : 19915009, PubMed : 23294003, PubMed : 25731159). In parallel, the IL6 signaling pathway induces the expression of two cytokine receptor signaling inhibitors, SOCS1 and SOCS3, which inhibit JAK and terminate the activity of the IL6 signaling pathway as a negative feedback loop (By similarity). Also activates the yes-associated protein 1 (YAP) and NOTCH pathways to control inflammation-induced epithelial regeneration, independently of STAT3 (By similarity). Acts as a receptor for the neuroprotective peptide humanin as part of a complex with IL27RA/WSX1 and CNTFR (PubMed : 19386761). Mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism (By similarity). Has a role in embryonic development (By similarity). Essential for survival of motor and sensory neurons and for differentiation of astrocytes (By similarity). Required for expression of TRPA1 in nociceptive neurons (By similarity). Required for the maintenance of PTH1R expression in the osteoblast lineage and for the stimulation of PTH-induced osteoblast differentiation (By similarity). Required for normal trabecular bone mass and cortical bone composition (By similarity).. Isoform 2. Binds to the soluble IL6 : sIL6R complex (hyper-IL6), thereby blocking IL6 trans-signaling. Inhibits sIL6R-dependent acute phase response (PubMed : 11121117, PubMed : 21990364, PubMed : 30279168). Also blocks IL11 cluster signaling through IL11R (PubMed : 30279168).

See full target information IL6ST

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GP130/CD130 antibody [BLR114H] - BSA free

Immunocytochemistry - Anti-GP130/CD130 antibody [BLR114H] - BSA free (AB314107)

Immunocytochemistry - Anti-GP130/CD130 antibody [BLR114H] - BSA free (AB314107)

Immunocytochemistry - Anti-GP130/CD130 antibody [BLR114H] - BSA free (AB314107)

Western blot - Anti-GP130/CD130 antibody [BLR114H] - BSA free (AB314107)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise