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AB91214

Anti-gp64 antibody [AcV5]

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(5 Publications)

Mouse Monoclonal TLS/FUS antibody. Suitable for WB and reacts with Autographa californica nucleopolyhedrovirus samples. Cited in 5 publications. Immunogen corresponding to Cell preparation containing gp64 protein.

View Alternative Names

GP67, ORF128, GP64, Major envelope glycoprotein, gp64

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AcV5

Isotype

IgG2b

Carrier free

No

Reacts with

Autographa californica nucleopolyhedrovirus

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab91214 reacts with the gp64 envelope protein of the baculovirus Autographa californica (AcMNPV).

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Autographa californica nucleopolyhedrovirus": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>ab91214 can be used in identifying virally-infected insect cells and biochemical analysis of the gp64 protein.</p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The gp64 also known as glycoprotein 64 is a viral envelope protein that mechanically facilitates the fusion and entry of viral particles into host cells. It has an approximate mass of 64 kDa. Gp64 is expressed on the surface of baculoviruses which are viruses commonly used in insect cell systems. It plays an important role in the infection process by mediating membrane fusion between the virus and the host cell ensuring the delivery of viral DNA into the host.
Biological function summary

Gp64 functions as a critical component in the lifecycle of baculoviruses. During the infection cycle it facilitates the binding and entry of viral particles acting as a fusion protein. Gp64 is a part of the larger viral envelope protein framework which helps the virus adapt and interact with the host cell's membrane. Its role extends beyond simple attachment as it initiates the processes leading to successful viral replication within the host cell.

Pathways

Gp64 participates in the endocytosis pathway important for viral entry into host cells. It interacts with cellular membrane proteins both directly and indirectly ensuring fusion and release of viral contents. Gp64 shares a functional relationship with other viral envelope proteins like those found in orthologous systems such as the vesicular stomatitis virus G protein. This interaction highlights its integral role in the viral infection process.

Gp64 is notably associated with vector systems used in therapies and vaccinations particularly in cancer treatments. While not directly linked to causing diseases its role in facilitating baculovirus entry has made it an important target in designing vectors for gene delivery. Gp64 interacts indirectly with proteins related to immune response and cell signaling during its use in therapeutic models providing potential pathways in treating disorders like cancer.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Class III viral fusion protein. Envelope phosphoglycoprotein which mediates the fusion of viral and host endosomal membranes leading to virus entry into the host cell. After receptor-mediated internalization of the virus, gp64 undergoes conformational change into a fusion-competent state at low pH, and the nucleocapsid is released into the cytoplasm after cell fusion. May also play role in budding.
See full target information GP64

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:7711 PubMed39231976

2024

EXTL3 and NPC1 are mammalian host factors for Autographa californica multiple nucleopolyhedrovirus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yuege Huang,Hong Mei,Chunchen Deng,Wei Wang,Chao Yuan,Yan Nie,Jia-Da Li,Jia Liu

International journal of molecular sciences 25: PubMed39201788

2024

Development of Fusion-Based Assay as a Drug Screening Platform for Nipah Virus Utilizing Baculovirus Expression Vector System.

Applications

Unspecified application

Species

Unspecified reactive species

Indah Permata Sari,Christopher Llynard D Ortiz,Lee-Wei Yang,Ming-Hsiang Chen,Ming-Der Perng,Tzong-Yuan Wu

BMC microbiology 20:26 PubMed32019519

2020

Identification of E2 with improved secretion and immunogenicity against CSFV in piglets.

Applications

Unspecified application

Species

Unspecified reactive species

Huiling Xu,Yanli Wang,Guangwei Han,Weihuan Fang,Fang He

PloS one 9:e105986 PubMed25221982

2014

Differential susceptibilities to BmNPV infection of two cell lines derived from the same silkworm ovarian tissues.

Applications

WB

Species

Unspecified reactive species

Jun Zhang,Xue-Mei Chen,Chun-Dong Zhang,Qian He,Zhan-Qi Dong,Ming-Ya Cao,Xiao-Long Dong,Cai-Xia Pan,Cheng Lu,Min-Hui Pan

Virology 125:432-44 PubMed6340331

1983

Monoclonal antibodies to baculovirus structural proteins: determination of specificities by Western blot analysis.

Applications

Unspecified application

Species

Unspecified reactive species

A W Hohmann,P Faulkner
View all publications

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