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AB68925

Anti-GPAM antibody

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(2 Publications)

Mouse Polyclonal GPAM antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GPAM.

View Alternative Names

GPAT1, KIAA1560, GPAM, GPAT-1

1 Images
Western blot - Anti-GPAM antibody (AB68925)
  • WB

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Western blot - Anti-GPAM antibody (AB68925)

All lanes:

Western blot - Anti-GPAM antibody (ab68925) at 1/500 dilution

Lane 1:

GPAM transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

Predicted band size: 94 kDa

Observed band size: 98 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GPAM.

Q9HCL2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPAM also known as glycerol-3-phosphate acyltransferase mitochondrial is an enzyme that plays an important role in lipid biosynthesis. This enzyme which consists of approximately 828 amino acids has a mass of around 92 kilodaltons (kDa). GPAM is mainly found in the mitochondria of cells and exhibits high expression levels in tissues such as adipose tissue liver and muscle. Its function involves the initial steps in the synthesis of glycerolipids by catalyzing the esterification of glycerol-3-phosphate with long-chain fatty acids.
Biological function summary

The enzyme influences important lipid metabolic processes across different tissues. GPAM initiates the triacylglycerol biosynthesis pathway by producing lysophosphatidic acid which is a precursor to phosphatidic acid. It does not form a direct complex with other proteins but it does interact with lipid metabolic processes and affects the overall homeostasis of lipids within the cell. It impacts energy storage in adipocytes and has a role in liver metabolic regulation.

Pathways

GPAM participates in the glycerolipid and glycerophospholipid metabolism pathways. It functions at the intersection of these pathways to regulate lipid accumulation and usage in cells especially in adipose tissue and liver. GPAM's activity connects with other enzymes such as diacylglycerol O-acyltransferase (DGAT) in the process of triacylglycerol synthesis and overall lipid metabolism.

GPAM has been linked to obesity and non-alcoholic fatty liver disease (NAFLD). Elevated enzyme activity can lead to increased lipid storage contributing to obesity while dysregulation can play a role in the development of fatty liver conditions. GPAM interacts with proteins like adiponectin where altered pathways can influence metabolic syndromes and associated complications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial membrane protein that catalyzes the essential first step of biosynthesis of glycerolipids such as triglycerides, phosphatidic acids and lysophosphatidic acids (PubMed : 18238778, PubMed : 19075029, PubMed : 36522428). Esterifies acyl-group from acyl-coenzyme A (acyl-CoA) to the sn-1 position of glycerol-3-phosphate, to produce lysophosphatidic acid (PubMed : 18238778). Has a narrow hydrophobic binding cleft that selects for a linear acyl chain (PubMed : 36522428). Catalytic activity is higher for substrates with a 16-carbon acyl chain (PubMed : 36522428).
See full target information GPAM
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Gastrointestinal and liver physiology 308:G198-205 PubMed25477377

2014

Hepatic AQP9 expression in male rats is reduced in response to PPARα agonist treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Janne Lebeck,Muhammad Umar Cheema,Mariusz T Skowronski,Søren Nielsen,Jeppe Praetorius

Proceedings of the National Academy of Sciences of 109:1667-72 PubMed22307628

2012

Glycerolipid signals alter mTOR complex 2 (mTORC2) to diminish insulin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chongben Zhang,Angela A Wendel,Matthew R Keogh,Thurl E Harris,Jie Chen,Rosalind A Coleman
View all publications
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