AB69990

Anti-GPAM antibody

Name: Anti-GPAM antibody (ab69990) | Abcam
Brand: Abcam Limited
SKU: AB69990
Rating: 3 (3 reviews)

(3 Reviews)

(6 Publications)

Rabbit Polyclonal GPAM antibody. Suitable for WB and reacts with Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human GPAM.

View Alternative Names

GPAT1, KIAA1560, GPAM, GPAT-1

1 Images

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Western blot - Anti-GPAM antibody (AB69990)

Lane 1:

Western blot - Anti-GPAM antibody (ab69990) at 1 µg/mL

Lane 2:

Western blot - Anti-GPAM antibody (ab69990) at 2 µg/mL

All lanes:

Rat brain tissue lysate at 15 µg

Predicted band size: 94 kDa

Observed band size: 80 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

Synthetic Peptide within Human GPAM. The exact immunogen used to generate this antibody is proprietary information.

Q9HCL2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPAM also known as glycerol-3-phosphate acyltransferase mitochondrial is an enzyme that plays an important role in lipid biosynthesis. This enzyme which consists of approximately 828 amino acids has a mass of around 92 kilodaltons (kDa). GPAM is mainly found in the mitochondria of cells and exhibits high expression levels in tissues such as adipose tissue liver and muscle. Its function involves the initial steps in the synthesis of glycerolipids by catalyzing the esterification of glycerol-3-phosphate with long-chain fatty acids.

Biological function summary

The enzyme influences important lipid metabolic processes across different tissues. GPAM initiates the triacylglycerol biosynthesis pathway by producing lysophosphatidic acid which is a precursor to phosphatidic acid. It does not form a direct complex with other proteins but it does interact with lipid metabolic processes and affects the overall homeostasis of lipids within the cell. It impacts energy storage in adipocytes and has a role in liver metabolic regulation.

Pathways

GPAM participates in the glycerolipid and glycerophospholipid metabolism pathways. It functions at the intersection of these pathways to regulate lipid accumulation and usage in cells especially in adipose tissue and liver. GPAM's activity connects with other enzymes such as diacylglycerol O-acyltransferase (DGAT) in the process of triacylglycerol synthesis and overall lipid metabolism.

GPAM has been linked to obesity and non-alcoholic fatty liver disease (NAFLD). Elevated enzyme activity can lead to increased lipid storage contributing to obesity while dysregulation can play a role in the development of fatty liver conditions. GPAM interacts with proteins like adiponectin where altered pathways can influence metabolic syndromes and associated complications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial membrane protein that catalyzes the essential first step of biosynthesis of glycerolipids such as triglycerides, phosphatidic acids and lysophosphatidic acids (PubMed : 18238778, PubMed : 19075029, PubMed : 36522428). Esterifies acyl-group from acyl-coenzyme A (acyl-CoA) to the sn-1 position of glycerol-3-phosphate, to produce lysophosphatidic acid (PubMed : 18238778). Has a narrow hydrophobic binding cleft that selects for a linear acyl chain (PubMed : 36522428). Catalytic activity is higher for substrates with a 16-carbon acyl chain (PubMed : 36522428).

See full target information GPAM

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 13:1049818 PubMed36588703

2022

Naringin attenuates fructose-induced NAFLD progression in rats through reducing endogenous triglyceride synthesis and activating the Nrf2/HO-1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sirinat Pengnet,Phinsuda Sumarithum,Nuttaphong Phongnu,Sakdina Prommaouan,Napapas Kantip,Ittipon Phoungpetchara,Wachirawadee Malakul

Antioxidants (Basel, Switzerland) 11: PubMed36421440

2022

-Fermented Ameliorates Metabolic-Syndrome-Like Pathological Conditions in Long-Term HFHFD-Fed Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Hua Wang,Seung-Ju Hwang,Kwang-Soo Shin,Dong-Woo Lim,Chang-Gue Son

Endocrine 75:92-107 PubMed34327606

2021

Chronic angiotensin receptor activation promotes hepatic triacylglycerol accumulation during an acute glucose challenge in obese-insulin-resistant OLETF rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jose A Godoy-Lugo,Max A Thorwald,David Y Hui,Akira Nishiyama,Daisuke Nakano,Jose G Soñanez-Organis,Rudy M Ortiz

Diabetologia 64:1642-1659 PubMed33770195

2021

Exercise training improves mitochondrial respiration and is associated with an altered intramuscular phospholipid signature in women with obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Amy E Mendham,Julia H Goedecke,Yingxu Zeng,Steen Larsen,Cindy George,Jon Hauksson,Melony C Fortuin-de Smidt,Alexander V Chibalin,Tommy Olsson,Elin Chorell

Journal of translational medicine 18:126 PubMed32169080

2020

Long noncoding RNA lncARSR promotes nonalcoholic fatty liver disease and hepatocellular carcinoma by promoting YAP1 and activating the IRS2/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Chi,Zheng Gong,He Xin,Ziwen Wang,Zhaoyu Liu

Obesity (Silver Spring, Md.) 22:1632-42 PubMed24500776

2014

Novel effects of rosiglitazone on SMAD2 and SMAD3 signaling in white adipose tissue of diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Marie-Soleil Beaudoin,Laelie A Snook,Alicia M Arkell,Amanda Stefanson,Zhongxiao Wan,Jeremy A Simpson,Graham P Holloway,David C Wright

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GPAM antibody

Western blot - Anti-GPAM antibody (AB69990)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise