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Rabbit Recombinant Monoclonal GPAM antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Not recommended
Tested
Not recommended
Mouse
Tested
Tested
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human, Mouse

Dilution info

-

Notes

-

Target data

Function

Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipids biosynthesis such as triglycerides, phosphatidic acids and lysophosphatidic acids.

Alternative names

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Rabbit Recombinant Monoclonal GPAM antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR29023-506

Purification technique

Affinity purification Protein A

Specificity

Unsuitable for human IHC-P

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

The enzyme influences important lipid metabolic processes across different tissues. GPAM initiates the triacylglycerol biosynthesis pathway by producing lysophosphatidic acid which is a precursor to phosphatidic acid. It does not form a direct complex with other proteins but it does interact with lipid metabolic processes and affects the overall homeostasis of lipids within the cell. It impacts energy storage in adipocytes and has a role in liver metabolic regulation.

Activity summary

GPAM also known as glycerol-3-phosphate acyltransferase mitochondrial is an enzyme that plays an important role in lipid biosynthesis. This enzyme which consists of approximately 828 amino acids has a mass of around 92 kilodaltons (kDa). GPAM is mainly found in the mitochondria of cells and exhibits high expression levels in tissues such as adipose tissue liver and muscle. Its function involves the initial steps in the synthesis of glycerolipids by catalyzing the esterification of glycerol-3-phosphate with long-chain fatty acids.

Pathways

GPAM participates in the glycerolipid and glycerophospholipid metabolism pathways. It functions at the intersection of these pathways to regulate lipid accumulation and usage in cells especially in adipose tissue and liver. GPAM's activity connects with other enzymes such as diacylglycerol O-acyltransferase (DGAT) in the process of triacylglycerol synthesis and overall lipid metabolism.

Associated diseases and disorders

GPAM has been linked to obesity and non-alcoholic fatty liver disease (NAFLD). Elevated enzyme activity can lead to increased lipid storage contributing to obesity while dysregulation can play a role in the development of fatty liver conditions. GPAM interacts with proteins like adiponectin where altered pathways can influence metabolic syndromes and associated complications.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-GPAM antibody [EPR29023-506] (ab322201), expandable thumbnail

    Western blot - Anti-GPAM antibody [EPR29023-506] (ab322201)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: spleen.

    Lanes 1-2 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 and lanes 3-5 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-GPAM antibody [EPR29023-506] (AB322201) at 1/1000 dilution

    Lane 1: Human fat tissue lysate at 20 µg

    Lane 2: Human spleen tissue lysate at 20 µg

    Lane 3: Mouse liver tissue lysate at 20 µg

    Lane 4: Mouse testis tissue lysate at 20 µg

    Lane 5: Mouse spleen tissue lysate at 20 µg

    Secondary

    Lanes 1 - 2: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Lanes 3 - 5: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Observed band size: 93 kDa, 36 kDa

    Exposure time: 15s

  • Western blot - Anti-GPAM antibody [EPR29023-506] (ab322201), expandable thumbnail

    Western blot - Anti-GPAM antibody [EPR29023-506] (ab322201)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the lower MW band at approximately 40 kDa is unknown.

    Exposure time: Lane 1: 114 seconds; Lanes 2-7: 37 seconds.

    All lanes: Western blot - Anti-GPAM antibody [EPR29023-506] (AB322201) at 1/1000 dilution

    Lane 1: NCI-H1975 (human adenocarcinoma lung epithelial cell) whole cell lysate at 20 µg

    Lane 2: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 7: 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Observed band size: 93 kDa

  • Western blot - Anti-GPAM antibody [EPR29023-506] (ab322201), expandable thumbnail

    Western blot - Anti-GPAM antibody [EPR29023-506] (ab322201)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the lower MW band at approximately 40 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-GPAM antibody [EPR29023-506] (AB322201) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HeLa transfected with siRNA specifically targeting GPAM whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Observed band size: 93 kDa, 36 kDa

    Exposure time: 114s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPAM antibody [EPR29023-506] (ab322201), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPAM antibody [EPR29023-506] (ab322201)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling GPAM with ab322201 at 1/500 (0.994 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression: weak staining on mouse spleen. The section was incubated with ab322201 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPAM antibody [EPR29023-506] (ab322201), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPAM antibody [EPR29023-506] (ab322201)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling GPAM with ab322201 at 1/500 (0.994 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse liver. The section was incubated with ab322201 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPAM antibody [EPR29023-506] (ab322201), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPAM antibody [EPR29023-506] (ab322201)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling GPAM with ab322201 at 1/500 (0.994 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse kidney. The section was incubated with ab322201 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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