Rabbit Recombinant Monoclonal GPAM antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Not recommended | Tested | Not recommended |
Mouse | Tested | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Mitochondrial membrane protein that catalyzes the essential first step of biosynthesis of glycerolipids such as triglycerides, phosphatidic acids and lysophosphatidic acids (PubMed:18238778, PubMed:19075029, PubMed:36522428). Esterifies acyl-group from acyl-coenzyme A (acyl-CoA) to the sn-1 position of glycerol-3-phosphate, to produce lysophosphatidic acid (PubMed:18238778). Has a narrow hydrophobic binding cleft that selects for a linear acyl chain (PubMed:36522428). Catalytic activity is higher for substrates with a 16-carbon acyl chain (PubMed:36522428).
GPAT1, KIAA1560, GPAM, GPAT-1
Rabbit Recombinant Monoclonal GPAM antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Unsuitable for human IHC-P
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
GPAM also known as glycerol-3-phosphate acyltransferase mitochondrial is an enzyme that plays an important role in lipid biosynthesis. This enzyme which consists of approximately 828 amino acids has a mass of around 92 kilodaltons (kDa). GPAM is mainly found in the mitochondria of cells and exhibits high expression levels in tissues such as adipose tissue liver and muscle. Its function involves the initial steps in the synthesis of glycerolipids by catalyzing the esterification of glycerol-3-phosphate with long-chain fatty acids.
The enzyme influences important lipid metabolic processes across different tissues. GPAM initiates the triacylglycerol biosynthesis pathway by producing lysophosphatidic acid which is a precursor to phosphatidic acid. It does not form a direct complex with other proteins but it does interact with lipid metabolic processes and affects the overall homeostasis of lipids within the cell. It impacts energy storage in adipocytes and has a role in liver metabolic regulation.
GPAM participates in the glycerolipid and glycerophospholipid metabolism pathways. It functions at the intersection of these pathways to regulate lipid accumulation and usage in cells especially in adipose tissue and liver. GPAM's activity connects with other enzymes such as diacylglycerol O-acyltransferase (DGAT) in the process of triacylglycerol synthesis and overall lipid metabolism.
GPAM has been linked to obesity and non-alcoholic fatty liver disease (NAFLD). Elevated enzyme activity can lead to increased lipid storage contributing to obesity while dysregulation can play a role in the development of fatty liver conditions. GPAM interacts with proteins like adiponectin where altered pathways can influence metabolic syndromes and associated complications.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: spleen.
Lanes 1-2 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 and lanes 3-5 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-GPAM antibody [EPR29023-506] (ab322201) at 1/1000 dilution
Lane 1: Human fat tissue lysate at 20 µg
Lane 2: Human spleen tissue lysate at 20 µg
Lane 3: Mouse liver tissue lysate at 20 µg
Lane 4: Mouse testis tissue lysate at 20 µg
Lane 5: Mouse spleen tissue lysate at 20 µg
Lanes 1 - 2: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Lanes 3 - 5: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 93 kDa, 36 kDa
Exposure time: 15s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the lower MW band at approximately 40 kDa is unknown.
Exposure time: Lane 1: 114 seconds; Lanes 2-7: 37 seconds.
All lanes: Western blot - Anti-GPAM antibody [EPR29023-506] (ab322201) at 1/1000 dilution
Lane 1: NCI-H1975 (human adenocarcinoma lung epithelial cell) whole cell lysate at 20 µg
Lane 2: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 20 µg
Lane 6: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Lane 7: 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 93 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the lower MW band at approximately 40 kDa is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-GPAM antibody [EPR29023-506] (ab322201) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: HeLa transfected with siRNA specifically targeting GPAM whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 93 kDa, 36 kDa
Exposure time: 114s
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling GPAM with ab322201 at 1/500 (0.994 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: weak staining on mouse spleen. The section was incubated with ab322201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling GPAM with ab322201 at 1/500 (0.994 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse liver. The section was incubated with ab322201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling GPAM with ab322201 at 1/500 (0.994 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse kidney. The section was incubated with ab322201 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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