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AB130413

Anti-GPATCH1 antibody

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(1 Publication)

Rabbit Polyclonal GPATCH1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GPATCH1 aa 450-650.

View Alternative Names

ECGP, GPATC1, GPATCH1, G patch domain-containing protein 1, Evolutionarily conserved G-patch domain-containing protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPATCH1 antibody (AB130413)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPATCH1 antibody (AB130413)

ab130413 at 1/200 dilution staining GPATCH1 in Paraffin-embedded Human kidney tissue by Immunohistochemistry.

Western blot - Anti-GPATCH1 antibody (AB130413)
  • WB

Unknown

Western blot - Anti-GPATCH1 antibody (AB130413)

All lanes:

Western blot - Anti-GPATCH1 antibody (ab130413) at 1/250 dilution

Lane 1:

RT4 lysates

Lane 2:

U251 MG lysates

Lane 3:

Human Plasma lysates

Lane 4:

Human Liver lysates

Lane 5:

Human Tonsil lysates

Predicted band size: 103 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GPATCH1 aa 450-650. The exact immunogen used to generate this antibody is proprietary information.

Q9BRR8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

ab130413 is a mono-specific antibody.
The antibody solution should be gently mixed before use.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPATCH1 also known as G-patch domain-containing protein 1 is a protein involved in RNA processing. Its molecular weight is approximately 95 kDa. This protein is found mainly in the nucleus and it shows high expression levels in various tissues including the brain and liver. GPATCH1 contains a conserved glycine-rich domain called the G-patch which is important for its interaction with other proteins involved in RNA metabolism.
Biological function summary

GPATCH1 plays an important role in RNA splicing which is important for the correct maturation of pre-mRNA into mRNA. It cooperates with other proteins in spliceosome complexes ensuring accurate removal of introns and joining of exons. GPATCH1 also interacts with helicases helping to resolve RNA secondary structures during the splicing process. This interaction contributes to maintaining efficiency and fidelity during RNA processing.

Pathways

GPATCH1 is linked to the RNA splicing and mRNA surveillance pathways. It associates with the spliceosome a complex of snRNPs and protein factors to carry out intron excision. GPATCH1 is also related to the UPF1 protein which plays a role in the nonsense-mediated mRNA decay pathway. This connection emphasizes its involvement in pathways that ensure the integrity of gene expression.

GPATCH1 has been implicated in neurodegenerative diseases and certain cancers. Alterations in its function may contribute to aberrant splicing events which are observed in conditions like Alzheimer's disease. Additionally GPATCH1 has connections with proteins such as SRSF1 which is known to influence cancer cell proliferation. These associations point to GPATCH1's potential role as a therapeutic target in related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information GPATCH1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 13:e7017 PubMed38457205

2024

Enhanced clinical outcomes with radiotherapy in diagnostically challenging intracranial plasmacytomas: Analysis of 190 cases.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Feng,Zongpu Zhang,Fufang Qiu,Zixiao Yang,Ji Xiong,Wei Zhu,Fangzhu Wan,Bobin Chen,Jiguang Wang,Yi Zhang,Wei Hua
View all publications
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Product promise

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