AB154969

Anti-GPBB antibody

Name: Anti-GPBB antibody (ab154969) | Abcam
Brand: Abcam Limited
SKU: AB154969
Price: 545 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(7 Publications)

Rabbit Polyclonal GPBB antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Glycogen phosphorylase, brain form aa 500 to C-terminus.

View Alternative Names

PYGB

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPBB antibody (AB154969)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPBB antibody (AB154969)

Immunohistochemical analysis of paraffin embedded u87 xenograft tissue stained for PYGB protein at cytosol with ab154969 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPBB antibody (AB154969)

Immunohistochemical analysis of paraffin embedded mouse fore brain tissue stained for GPBB protein at cytosol with ab154969 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Supplier Data

Western blot - Anti-GPBB antibody (AB154969)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-GPBB antibody (ab154969) at 1/1000 dilution

All lanes:

Mouse brain tissue lysates at 50 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 96 kDa

false

Supplier Data

Western blot - Anti-GPBB antibody (AB154969)

Samples were separated by 7.5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-GPBB antibody (ab154969) at 1/1000 dilution

Lane 1:

SK-MEL-5 whole cell extracts at 30 µg

Lane 2:

HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 97 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Glycogen phosphorylase, brain form aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P11216

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein generally known as GPBB (glycogen phosphorylase brain form) plays a critical role in glucose metabolism. An alternate name for this protein is brain-type glycogen phosphorylase. The GPBB protein weighs approximately 97 kDa. It is mainly expressed in the brain but also present in skeletal and cardiac muscle tissues. This enzyme catalyzes the breakdown of glycogen into glucose-1-phosphate an essential step in energy production particularly during periods of increased cellular energy demand.

Biological function summary

GPBB contributes significantly to cellular energy homeostasis. It often partners with AMP-activated protein kinase (AMPK) among other proteins acting as a part of cellular complexes that regulate energy metabolism. GPBB is essential in maintaining the balance between glycogen synthesis and degradation ensuring that glucose levels rise during energy-demanding activities. This regulation supports proper brain function especially in neurons which heavily rely on glucose as an energy source.

Pathways

GPBB is fundamental in the glycogenolysis pathway which mobilizes glucose from glycogen reserves during metabolic needs. GPBB also relates to the insulin signaling pathway influencing how cells respond to insulin a protein vital for glucose uptake and metabolism. Through these pathways GPBB interacts with other proteins like glycogen synthase and phosphoglucomutase establishing a network that carefully coordinates metabolic responses to varying energy availability.

GPBB has significant implications in cardiac ischemia and neurodegenerative disorders such as Alzheimer's disease. During cardiac ischemia elevated levels of GPBB often indicate heart damage given its role in glycogen breakdown in cardiac muscle cells. In Alzheimer's disease abnormal GPBB activity might affect neuronal energy balance contributing to the disease's progression. Understanding these connections is important for developing therapeutic approaches targeting metabolic pathways involving GPBB.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Glycogen phosphorylase that regulates glycogen mobilization (PubMed : 27402852). Phosphorylase is an important allosteric enzyme in carbohydrate metabolism (PubMed : 3346228). Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates (PubMed : 3346228). However, all known phosphorylases share catalytic and structural properties (PubMed : 3346228).

See full target information Glycogen phosphorylase, brain form

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37046993

2023

Deciphering the Polyglucosan Accumulation Present in Lafora Disease Using an Astrocytic Cellular Model.

Applications

Unspecified application

Species

Unspecified reactive species

Mireia Moreno-Estellés,Ángela Campos-Rodríguez,Carla Rubio-Villena,Lorena Kumarasinghe,Maria Adelaida Garcia-Gimeno,Pascual Sanz

Oncology letters 24:244 PubMed35761940

2022

Analysis of the expression, function and signaling of glycogen phosphorylase isoforms in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyu Jiang,Shuyan Liu,Tingzhi Deng,Yang Yang,Yin Zhang

Frontiers in microbiology 11:557039 PubMed33329421

2020

Glycogen Phosphorylase: A Drug Target of Amino Alcohols in , Predicted by a Computer-Aided Method.

Applications

Unspecified application

Species

Unspecified reactive species

Congshan Liu,Jianhai Yin,Wei Hu,Haobing Zhang

Neuroscience bulletin 36:1513-1523 PubMed33048310

2020

Dynamic Variations in Brain Glycogen are Involved in Modulating Isoflurane Anesthesia in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ze Fan,Zhihao Zhang,Shiyi Zhao,Yuanyuan Zhu,Dong Guo,Bo Yang,Lixia Zhuo,Jiao Han,Rui Wang,Zongping Fang,Hailong Dong,Yan Li,Lize Xiong

Technology in cancer research & treatment 19:1533033820926592 PubMed32462986

2020

PYGB Promoted Tumor Progression by Regulating Wnt/β-Catenin Pathway in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Boning Xia,Ke Zhang,Chang Liu

Molecular medicine reports 18:3800-3808 PubMed30106110

2018

Silencing of PYGB suppresses growth and promotes the apoptosis of prostate cancer cells via the NF‑κB/Nrf2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Wang,Gang Han,Qinghong Liu,Wenyuan Zhang,Jinshan Wang

Molecular medicine reports 18:715-722 PubMed29845265

2018

PYGB siRNA inhibits the cell proliferation of human osteosarcoma cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Shuwei Zhang,Yichi Zhou,Yuanyu Zha,Yang Yang,Linlong Wang,Jingfeng Li,Wei Jin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GPBB antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPBB antibody (AB154969)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPBB antibody (AB154969)

Western blot - Anti-GPBB antibody (AB154969)

Western blot - Anti-GPBB antibody (AB154969)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise