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AB251810

Anti-GPBB antibody

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(1 Publication)

Rabbit Polyclonal GPBB antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Glycogen phosphorylase, brain form aa 650 to C-terminus.

View Alternative Names

PYGB

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPBB antibody (AB251810)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPBB antibody (AB251810)

Paraffin-embedded human prostate tissue stained for GPBB using ab251810 at 1/1000 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-GPBB antibody (AB251810)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GPBB antibody (AB251810)

PFA-fixed, Triton X-100 permeabilized PC-3 (human prostate adenocarcinoma cell line) cells stained for GPBB (green) using ab251810 at 4 µg/ml in ICC/IF.

Western blot - Anti-GPBB antibody (AB251810)
  • WB

Supplier Data

Western blot - Anti-GPBB antibody (AB251810)

All lanes:

Western blot - Anti-GPBB antibody (ab251810) at 0.4 µg/mL

Lane 1:

SK-MEL-30 cell line

Lane 2:

Human epithelial cell line from cervix adenocarcinoma (HeLa cell line)

Predicted band size: 96 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Glycogen phosphorylase, brain form aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P11216

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein generally known as GPBB (glycogen phosphorylase brain form) plays a critical role in glucose metabolism. An alternate name for this protein is brain-type glycogen phosphorylase. The GPBB protein weighs approximately 97 kDa. It is mainly expressed in the brain but also present in skeletal and cardiac muscle tissues. This enzyme catalyzes the breakdown of glycogen into glucose-1-phosphate an essential step in energy production particularly during periods of increased cellular energy demand.
Biological function summary

GPBB contributes significantly to cellular energy homeostasis. It often partners with AMP-activated protein kinase (AMPK) among other proteins acting as a part of cellular complexes that regulate energy metabolism. GPBB is essential in maintaining the balance between glycogen synthesis and degradation ensuring that glucose levels rise during energy-demanding activities. This regulation supports proper brain function especially in neurons which heavily rely on glucose as an energy source.

Pathways

GPBB is fundamental in the glycogenolysis pathway which mobilizes glucose from glycogen reserves during metabolic needs. GPBB also relates to the insulin signaling pathway influencing how cells respond to insulin a protein vital for glucose uptake and metabolism. Through these pathways GPBB interacts with other proteins like glycogen synthase and phosphoglucomutase establishing a network that carefully coordinates metabolic responses to varying energy availability.

GPBB has significant implications in cardiac ischemia and neurodegenerative disorders such as Alzheimer's disease. During cardiac ischemia elevated levels of GPBB often indicate heart damage given its role in glycogen breakdown in cardiac muscle cells. In Alzheimer's disease abnormal GPBB activity might affect neuronal energy balance contributing to the disease's progression. Understanding these connections is important for developing therapeutic approaches targeting metabolic pathways involving GPBB.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Glycogen phosphorylase that regulates glycogen mobilization (PubMed : 27402852). Phosphorylase is an important allosteric enzyme in carbohydrate metabolism (PubMed : 3346228). Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates (PubMed : 3346228). However, all known phosphorylases share catalytic and structural properties (PubMed : 3346228).
See full target information Glycogen phosphorylase, brain form
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioscience reports 40: PubMed32880385

2020

Identification and validation of a prognostic index based on a metabolic-genomic landscape analysis of ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qun-Feng Zhang,Yu-Kun Li,Chang-Ye Chen,Xiao-di Zhang,Lu Cao,Fei-Fei Quan,Xin Zeng,Juan Wang,Jue Liu
View all publications
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Product promise

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