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AB326055

Anti-GPC2 antibody [EPR30350-587] - BSA and Azide free

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Rabbit Recombinant Monoclonal GPC2 antibody. Carrier free. Suitable for WB, I-ELISA and reacts with Human, Recombinant full length protein - Human samples.

View Alternative Names

Glypican-2, GPC2

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ELISA - Anti-GPC2 antibody [EPR30350-587] - BSA and Azide free (AB326055)
  • ELISA

Supplier Data

ELISA - Anti-GPC2 antibody [EPR30350-587] - BSA and Azide free (AB326055)

This data was developed using ab324954, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab324954 at 1000-0 ng/ml. The Secondary antibody used was Peroxidase AffiniPure Goat Anti-Rabbit IgG, Fc fragment specific at 1 : 20000 dilution dilution.

Antigen : Human GPC2 protein, Human GPC6 protein, Human GPC1 protein.

Antigen concentration : 1000 ng/ml

This antibody does not cross-react with human GPC1 and GPC6.

Western blot - Anti-GPC2 antibody [EPR30350-587] - BSA and Azide free (AB326055)
  • WB

Supplier Data

Western blot - Anti-GPC2 antibody [EPR30350-587] - BSA and Azide free (AB326055)

This data was developed using ab324954, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : HeLa 293T ARPE-19 (PMID : 34195677).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 34195677; PMID : 34337560; PMID : 12084985).

The identity of the higher MW bands observed in lanes 1–2 are most likely glycosylated forms of GPC2 (PMID : 34337560).

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1 : 136 seconds; Lanes 2-5 : 70 seconds.

All lanes:

Western blot - Anti-GPC2 antibody [EPR30350-587] (<a href='/en-us/products/primary-antibodies/gpc2-antibody-epr30350-587-ab324954'>ab324954</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 48 µg

Lane 2:

IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 48 µg

Lane 3:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 48 µg

Lane 4:

293T (human embryonic kidney epithelial cell) whole cell lysate at 48 µg

Lane 5:

ARPE-19 (human eye epithelial cell) whole cell lysate at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 62 kDa,80-300 kDa,36 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30350-587

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Unsuitable for WB in human tissues.

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Reactivity data

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Product details

ab326055 is the carrier-free version of ab324954

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface proteoglycan that bears heparan sulfate. May fulfill a function related to the motile behaviors of developing neurons (By similarity).
See full target information GPC2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com