Anti-GPC2 antibody [EPR30350-627]

• Flow Cyt

Lab

Flow Cytometry - Anti-GPC2 antibody [EPR30350-627] (AB324378)

Flow cytometry overlay histogram of Negative control : HeLa (human cervical adenocarcinoma epithelial cells, Left) and IMR-32 (human neuroblastoma neuroblasts, Right) labelling GPC2 with ab324378 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Secondary antibody details, Goat anti-Rabbit IgG (Alexa Fluor^® 647 (ab150083), at 1/5000 dilution was used as the secondary antibody. Gated on viable cells.

• Flow Cyt

Lab

Flow Cytometry - Anti-GPC2 antibody [EPR30350-627] (AB324378)

Flow cytometry overlay histogram of WERI-Rb-1 (human Eye Retina grape-like clusters of round cells) labelling GPC2 with ab324378 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Secondary antibody details, Goat anti-Rabbit IgG (Alexa Fluor^® 647 (ab150083), at 1/5000 dilution was used as the secondary antibody. Gated on viable cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPC2 antibody [EPR30350-627] (AB324378)

Immunohistochemical analysis of paraffin-embedded (A) IMR-32 (human neuroblastoma neuroblast) cell pellet and (B) HeLa (human cervical adenocarcinoma epithelial cell) cell pellet labeling GPC2 with ab324378 at 1/200 (2.555 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Positive staining on (A) IMR-32 cell pellet, no staining on (B) HeLa cell pellet.
The primary antibody was incubated overnight at 4°C.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-GPC2 antibody [EPR30350-627] (AB324378)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human testis tissue staining GPC2 with ab324378 at a 1/200 dilution, ab323317 anti-Oligodendrocyte Specific Protein used at 1/500 dilution and ab323802 anti-MAGEA4 used at a 1/1000 dilution.

Panel A : anti-GPC2 (green; Opal™520), anti-Oligodendrocyte Specific Protein (magenta; Opal™690) and anti-MAGEA4 (gray; Opal™570) on human testis.

Panel B : anti-GPC2 staining early spermatids in human testis.

Panel C : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in human testis.

Panel D : anti-MAGEA4 staining spermatogonia in human testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324378, ab323317 and ab323802 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPC2 antibody [EPR30350-627] (AB324378)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling GPC2 with ab324378 at 1/200 (2.555 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Positive staining on human testis.
The primary antibody was incubated overnight at 4°C.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPC2 antibody [EPR30350-627] (AB324378)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling GPC2 with ab324378 at 1/200 (2.555 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on human colon (PMID : 28739923).
The primary antibody was incubated overnight at 4°C.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPC2 antibody [EPR30350-627] (AB324378)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GPC2 with ab324378 at 1/200 (2.555 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Negative control : No staining on human kidney (PMID : 28739923).
The primary antibody was incubated overnight at 4°C.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• I-ELISA

Supplier Data

Indirect ELISA - Anti-GPC2 antibody [EPR30350-627] (AB324378)

Indirect ELISA analysis of ab324378 at 1000-0 ng/ml. The Secondary antibody used was Peroxidase AffiniPure Goat Anti-Rabbit IgG, Fc fragment specific at 1 : 20000 dilution dilution.

Antigen : Human GPC2 protein, Human GPC6 protein, Human GPC1 protein.

Antigen concentration : 1000 ng/ml

This antibody does not cross-react with human GPC1 and GPC6.