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Rabbit Polyclonal GPCR C5L2 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human C5AR2 conjugated to Keyhole Limpet Haemocyanin.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human C5AR2 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9P296
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested

Tested
Tested

Species

Human

Dilution info

10 µg/mL

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target data

Function

Receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides and also for their dearginated forms ASP/C3adesArg, C4adesArg and C5adesArg respectively. Couples weakly to G(i)-mediated signaling pathways.

Alternative names

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Rabbit Polyclonal GPCR C5L2 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human C5AR2 conjugated to Keyhole Limpet Haemocyanin.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human C5AR2 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9P296
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-80°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

Biological function summary

C5L2 participates in immune response modulation lipid metabolism and inflammation resolution. This receptor does not operate as part of a known complex but plays a critical role in complement cascade regulation. It binds complement component C5a and its inactivated form C5a des-Arg which is a significant function distinguishing it from other receptors. C5L2 may act to sequester these components thereby limiting excessive inflammatory responses and sustaining homeostasis.

Activity summary

The G-protein-coupled receptor C5L2 also known as GPR77 is a member of the GPCR family a large group of cell surface receptors essential for signal transduction. It is a glycoprotein with a molecular mass of around 39 kDa. This receptor does not share typical signaling properties with classical GPCRs as it lacks G-protein activation function. Instead it acts as a decoy receptor. C5L2 is widely expressed in adipose tissue macrophages and other immune cells with an abundant presence in the lungs liver and spleen.

Pathways

This receptor is involved in the complement and the adipokine signaling pathways. C5L2 interacts within the complement system to mediate the immune response functioning alongside other components such as C5aR1. Its presence in the adipokine pathway suggests a role in energy metabolism hinting at the regulation of glucose and lipid homeostasis in adipose tissues and potentially engaging interactions with proteins like adiponectin.

Associated diseases and disorders

C5L2 associates with conditions like sepsis and metabolic syndrome. In sepsis C5L2's regulation of the complement pathway influences inflammatory responses and outcomes. The association of C5L2 with metabolic syndrome implicates its involvement in dysregulated adipokine signaling suggesting a direct relationship with proteins such as C5aR1 in these contexts. Understanding C5L2's function provides insights into therapeutic targets for these diseases.

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1 product image

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR C5L2 antibody (ab116709), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR C5L2 antibody (ab116709)

    Human brain, cortex (formalin fixed, paraffin embedded tissue) stained with ab116709 at 10 μg/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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