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AB116709

Anti-GPCR C5L2 antibody

5

(1 Review)

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(1 Publication)

Rabbit Polyclonal GPCR C5L2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human C5AR2 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

C5L2, GPR77, C5AR2, C5a anaphylatoxin chemotactic receptor 2, Complement component 5a receptor 2, G-protein coupled receptor 77

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR C5L2 antibody (AB116709)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR C5L2 antibody (AB116709)

Human brain, cortex (formalin fixed, paraffin embedded tissue) stained with ab116709 at 10 μg/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human C5AR2 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9P296

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody [EPR22301-103] (AB225732)

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Secondary Antibodies

AB150077

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Primary Antibodies

AB32059

Anti-cIAP2 antibody [E40]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP2 antibody [E40] (AB32059)

  • 4
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The G-protein-coupled receptor C5L2 also known as GPR77 is a member of the GPCR family a large group of cell surface receptors essential for signal transduction. It is a glycoprotein with a molecular mass of around 39 kDa. This receptor does not share typical signaling properties with classical GPCRs as it lacks G-protein activation function. Instead it acts as a decoy receptor. C5L2 is widely expressed in adipose tissue macrophages and other immune cells with an abundant presence in the lungs liver and spleen.
Biological function summary

C5L2 participates in immune response modulation lipid metabolism and inflammation resolution. This receptor does not operate as part of a known complex but plays a critical role in complement cascade regulation. It binds complement component C5a and its inactivated form C5a des-Arg which is a significant function distinguishing it from other receptors. C5L2 may act to sequester these components thereby limiting excessive inflammatory responses and sustaining homeostasis.

Pathways

This receptor is involved in the complement and the adipokine signaling pathways. C5L2 interacts within the complement system to mediate the immune response functioning alongside other components such as C5aR1. Its presence in the adipokine pathway suggests a role in energy metabolism hinting at the regulation of glucose and lipid homeostasis in adipose tissues and potentially engaging interactions with proteins like adiponectin.

C5L2 associates with conditions like sepsis and metabolic syndrome. In sepsis C5L2's regulation of the complement pathway influences inflammatory responses and outcomes. The association of C5L2 with metabolic syndrome implicates its involvement in dysregulated adipokine signaling suggesting a direct relationship with proteins such as C5aR1 in these contexts. Understanding C5L2's function provides insights into therapeutic targets for these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a, stimulating chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production (PubMed : 11773063). Also acts as a receptor for dearginated forms of C3a, C4a and C5a anaphylatoxin peptides (ASP/C3adesArg, C4adesArg and C5adesArg, respectively) (PubMed : 12540846, PubMed : 15833747, PubMed : 19615750). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed : 12540846). C5AR1 is coupled to G(i)/G(o) (GNAI1 or GNAO1) G alpha proteins and mediates inhibition of adenylate cyclase (PubMed : 12540846).
See full target information C5AR2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Hereditas 162:104 PubMed40514721

2025

Astragaloside IV regulates FOXM1 deubiquitination to ameliorate trophoblast damage caused by high glucose.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Li,Xiaofang Zhao
View all publications
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Product promise

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