AB188977

Anti-GPCR GPR1 antibody

Name: Anti-GPCR GPR1 antibody (ab188977) | Abcam
Brand: Abcam Limited
SKU: AB188977

(1 Review)

(2 Publications)

Rabbit Polyclonal GPCR GPR1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CMKLR2.

View Alternative Names

GPR1, CMKLR2, Chemerin-like receptor 2, Chemerin chemokine-like receptor 2, Chemokine-like receptor 2, G-protein coupled receptor 1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR1 antibody (AB188977)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR1 antibody (AB188977)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human kidney tissue labelling GPCR GPR1 with ab188977 at 20 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human CMKLR2. The exact immunogen used to generate this antibody is proprietary information.

P46091

Specificity

BLAST analysis of the peptide immunogen showed no homology with other human proteins, except LONRF2 (47%).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "15-20 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPR1 a G protein-coupled receptor (GPCR) is an integral membrane protein that plays a role in cellular signaling. Known alternatively as G protein-coupled receptor 1 its mass is about 41 kDa. GPR1 localizes in the cell membrane primarily expressed in tissues like the brain and kidney. Its function involves interaction with specific ligands leading to the activation of G-proteins and the downstream signaling cascade. This makes it an important player in transmitting extracellular signals to the interior of the cell.

Biological function summary

GPR1 takes part in inflammatory response and metabolism regulation. It does not appear to form part of a larger protein complex functioning independently in the pathways it influences. GPR1's role in attracting immune cells to sites of inflammation is significant as it interacts with chemokines to mediate leucocyte migration. In cellular metabolism GPR1 helps maintain energy balance by modulating pathways linked to energy and nutrient availability.

Pathways

GPR1 interacts with the MAPK signaling pathway and the chemokine-mediated signal transduction. These pathways involve proteins like ERK1/2 and CCR1 which help transmit signals that affect cellular responses such as growth differentiation or immune cell trafficking. GPR1 influences these pathways through ligand-binding leading to a cascade of intracellular events that impact cell function and communication.

GPR1 is associated with inflammation-related diseases and metabolic disorders. For instance its role in inflammation pathways links it to conditions like rheumatoid arthritis. It may also contribute to obesity by affecting energy regulation pathways. The protein demonstrates interaction with other receptors like CCR5 in these diseases which facilitates a better understanding of its involvement in pathological states. Researchers are exploring GPR1 as a potential therapeutic target given its role in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for chemoattractant adipokine chemerin/RARRES2 suggesting a role for this receptor in the regulation of inflammation and energy homesotasis (PubMed : 18165312, PubMed : 27716822). Signals mainly via beta-arrestin pathway. Binding of RARRES2 activates weakly G proteins, calcium mobilization and MAPK1/MAPK3 (ERK1/2) phosphorylation too (PubMed : 27716822). Also acts as a receptor for TAFA1, mediates its effects on neuronal stem-cell proliferation and differentiation via the activation of ROCK/ERK and ROCK/STAT3 signaling pathway (By similarity).. (Microbial infection) Coreceptor for HIV-1.

See full target information CMKLR2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 20: PubMed31404950

2019

Expression of Chemerin and Its Receptors in the Porcine Hypothalamus and Plasma Chemerin Levels during the Oestrous Cycle and Early Pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Smolinska,Marta Kiezun,Kamil Dobrzyn,Edyta Rytelewska,Katarzyna Kisielewska,Marlena Gudelska,Ewa Zaobidna,Krystyna Bogus-Nowakowska,Joanna Wyrebek,Kinga Bors,Grzegorz Kopij,Barbara Kaminska,Tadeusz Kaminski

Oncotarget 8:95135-95151 PubMed29221117

2017

Inhibition of chemerin/CMKLR1 axis in neuroblastoma cells reduces clonogenicity and cell viability and impairs tumor growth .

Applications

IHC-P

Species

Human

Conny Tümmler,Igor Snapkov,Malin Wickström,Ugo Moens,Linda Ljungblad,Lotta Helena Maria Elfman,Jan-Olof Winberg,Per Kogner,John Inge Johnsen,Baldur Sveinbjørnsson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GPCR GPR1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR1 antibody (AB188977)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise