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AB131117

Anti-GPCR GPR101 antibody

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(1 Publication)

Rabbit Polyclonal GPCR GPR101 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GPR101.

View Alternative Names

Probable G-protein coupled receptor 101, GPR101

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR101 antibody (AB131117)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR101 antibody (AB131117)

Immunofluorescence analysis of HeLa cells labelling GPCR GPR101 with ab131117 at a 1/100 dilution. The image on the right is treated with the synthesized peptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR101 antibody (AB131117)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR101 antibody (AB131117)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tubules labelling GPCR GPR101 with ab131117 at 10 μg/ml.

Western blot - Anti-GPCR GPR101 antibody (AB131117)
  • WB

Unknown

Western blot - Anti-GPCR GPR101 antibody (AB131117)

All lanes:

Western blot - Anti-GPCR GPR101 antibody (ab131117) at 1/500 dilution

Lane 1:

COS-7 (African green monkey kidney fibroblast-like cell line) cell extract

Lane 2:

COS-7 (African green monkey kidney fibroblast-like cell line) cell extract with synthesized peptide

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, African green monkey

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human GPR101. The exact immunogen used to generate this antibody is proprietary information.

Q96P66

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPR101 also known as G-protein coupled receptor 101 is a complex transmembrane protein that belongs to the GPCR family. As a GPCR it plays an important role in signal transduction by interacting with G-proteins upon activation by a ligand. This receptor has a molecular mass of approximately 56.4 kDa. GPR101 is expressed in variable levels across several human tissues with high expression noted in the brain particularly in the pituitary and hippocampus regions.
Biological function summary

The role of GPR101 involves promoting cellular responses to external signals leading to changes in cellular activity. It does not typically associate with larger protein complexes but interacts directly with G-proteins to mediate its functions. Research suggests GPR101 predominantly influences brain signaling pathways possibly affecting neurological processes and development.

Pathways

GPR101 integrates into key signaling routes by facilitating the activation of adenylate cyclase through G-protein signaling. This pathway plays a significant role in the control of cyclic AMP (cAMP) levels an important secondary messenger involved in several cellular processes. It shares pathway space with other GPCRs such as GPR161 which also influences cAMP signaling. Through these pathways GPR101 contributes to the regulation of neuronal signaling and potential growth factors.

GPR101 has been linked to certain growth disorders particularly gigantism. Abnormal expression or mutations in this receptor are associated with excessive growth hormone release. It is also connected to pituitary adenomas where its overexpression might contribute to tumor pathogenesis. Other GPCRs like GPR4 may also display dysregulation in similar conditions indicating a network of GPCR involvement in such disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Orphan receptor.
See full target information GPR101
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 13:20738-20747 PubMed34453028

2021

Apatinib inhibits the proliferation of gastric cancer cells via the AKT/GSK signaling pathway .

Applications

Unspecified application

Species

Unspecified reactive species

Yi Chen,Nan Chen,Jin Xu,Xindong Wang,Xiaowei Wei,Cuiju Tang,Zhong Duanmu,Junfeng Shi
View all publications
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Product promise

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