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AB136262

Anti-GPCR GPR116 antibody

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(5 Publications)

Rabbit Polyclonal GPCR GPR116 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human ADGRF5 aa 1-100.

View Alternative Names

GPR116, KIAA0758, ADGRF5, Adhesion G protein-coupled receptor F5, G-protein coupled receptor 116

1 Images
Western blot - Anti-GPCR GPR116 antibody (AB136262)
  • WB

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Western blot - Anti-GPCR GPR116 antibody (AB136262)

All lanes:

Western blot - Anti-GPCR GPR116 antibody (ab136262) at 1/500 dilution

All lanes:

HeLa cell lysate at 30 µg

Predicted band size: 149 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ADGRF5 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q8IZF2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab136262 was affinity-purified from rabbit antiserum by immunogenic peptide affinity-chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPCR GPR116 also known as adhesion G protein-coupled receptor F5 is a receptor protein that belongs to the large family of adhesion GPCRs. The GPR116 protein has an approximate mass of 140 kDa. It plays a role in regulating G protein signaling and interacts with other cellular proteins through its extracellular and intracellular domains. GPR116 is expressed in multiple tissues with high expression levels noted in endothelial cells lungs and adipose tissue.
Biological function summary

This receptor is involved in important processes like maintaining pulmonary surfactant homeostasis and regulating vascular integrity. GPR116 does not function alone but as part of larger receptor complexes interacting with various other proteins and molecules at the cellular membrane. Its activity influences the balance of signals within the cell impacting cellular responses and activities.

Pathways

GPR116 is an important component in the surfactant and vascular maintenance pathways. It is associated with the phosphatidylinositol signaling system and can influence phospholipase C activity. GPR116 also interacts with other proteins like thrombin which assists in mediating inflammatory responses via G protein-coupled signal transduction.

Dysfunction of GPR116 has implications in respiratory conditions like pulmonary alveolar proteinosis and cardiovascular diseases such as hypertension. In these disease contexts GPR116's relationship with proteins like surfactant protein A and B shows its role in regulating respiratory and cardiovascular conditions. Understanding these interactions might offer insights into targeting GPR116 for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adhesion G protein-coupled receptor (PubMed : 28570277). In alveolar type II (ATII or AT2) cells, required for normal lung surfactant homeostasis (PubMed : 28570277). Modulation of both surfactant secretion and uptake by ATII cells is mediated by the downstream activation of GNAQ/GNA11 proteins and may be a consequence of increased cortical F-actin assembly induced by ADGRF5 activation (PubMed : 28570277). In the kidney, may play a role in the regulation of acid excretion into the primary urine, possibly by regulating the surface expression of V-ATPase proton pump (By similarity). As a receptor for soluble FNDC4 (sFNDC4), required for proper systemic glucose tolerance, specifically sensitizing white adipose tissue to insulin. Plays also a role in sFNDC4-induced decrease of local inflammation in white adipose tissue (PubMed : 34016966).
See full target information ADGRF5
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Clinical nutrition (Edinburgh, Scotland) 43:2221-2233 PubMed39173437

2024

FNDC4 reduces hepatocyte inflammatory cell death via AMPKα in metabolic dysfunction-associated steatotic liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriela Neira,Sara Becerril,Víctor Valentí,Rafael Moncada,Victoria Catalán,Javier Gómez-Ambrosi,Inmaculada Colina,Camilo Silva,Javier Escalada,Gema Frühbeck,Amaia Rodríguez

Cell & bioscience 13:51 PubMed36895027

2023

GPR116 receptor regulates the antitumor function of NK cells via Gαq/HIF1α/NF-κB signaling pathway as a potential immune checkpoint.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Guo,Chenxu Jin,Yaoxin Gao,Haizhen Lin,Li Zhang,Ying Zhou,Jie Yao,Yixin Duan,Yaojun Ren,Xinhui Hui,Yujia Ge,Renzheng Yang,Wenzheng Jiang

Nature communications 12:2999 PubMed34016966

2021

Orphan GPR116 mediates the insulin sensitizing effects of the hepatokine FNDC4 in adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Anastasia Georgiadi,Valeria Lopez-Salazar,Rabih El- Merahbi,Rhoda Anane Karikari,Xiaochuan Ma,André Mourão,Katarina Klepac,Lea Bühler,Ana Jimena Alfaro,Isabell Kaczmarek,Adam Linford,Madeleen Bosma,Olga Shilkova,Olli Ritvos,Nobuhiro Nakamura,Shigehisa Hirose,Maximilian Lassi,Raffaele Teperino,Juliano Machado,Marcel Scheideler,Arne Dietrich,Arie Geerlof,Annette Feuchtinger,Andreas Blutke,Katrin Fischer,Timo Dirk Müller,Katharina Kessler,Torsten Schöneberg,Doreen Thor,Silke Hornemann,Michael Kruse,Peter Nawroth,Olga Pivovarova-Ramich,Andreas Friedrich Hermann Pfeiffer,Michael Sattler,Matthias Blüher,Stephan Herzig

ACS chemical neuroscience 12:990-997 PubMed33683115

2021

Phoenixin-20 Prevents ox-LDL-Induced Attachment of Monocytes to Human Aortic Endothelial Cells (HAECs): A Protective Implication in Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolan Wei,Huasong Lin,Biyue Zhang,Mimi Li,Yafang Chen,Yali Huang,Jinying Zhang,Yingxia Yang,Zeming Guo,Weiwei Li,Lichao Ye,Ruoting Lin

European review for medical and pharmacological sciences 23:6119-6130 PubMed31364112

2019

MiR-511-5p functions as a tumor suppressor and a predictive of prognosis in colorectal cancer by directly targeting GPR116.

Applications

Unspecified application

Species

Unspecified reactive species

C Wang,H-Q Fan,Y-W Zhang
View all publications
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