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AB288416

Anti-GPCR GPR119 antibody [Gipg013]

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Rabbit Recombinant Monoclonal GPCR GPR119 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GPR119.

View Alternative Names

Glucose-dependent insulinotropic receptor, G-protein coupled receptor 119, GPR119

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR119 antibody [Gipg013] (AB288416)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR119 antibody [Gipg013] (AB288416)

Immunofluoresence staining of fixed MDA-MB-231 cells with ab288416.

Immunofluorescence analysis of paraformaldehyde fixed MDA-MB-231 cells, permeabilized with 0.15% Triton stained ab288416 at 10 μg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 μg/ml), showing cytoplasmic staining with additional puncta. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom ab288416, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor® 488 secondary antibody.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Gipg013

Isotype

IgG

Light chain type

lambda

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GPR119.

Q8TDV5

Specificity

The original has a Ki value of 7 nM and a Kd value of 6.8 nM for human GIPr.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Proclin 300 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPR119 also known as Glucose-dependent insulinotropic receptor is a G protein-coupled receptor (GPCR) that plays an essential role in metabolic regulation. With a mass of approximately 51 kDa it is expressed mainly in pancreatic beta cells and intestinal enteroendocrine cells. This receptor senses dietary lipids and promotes insulin secretion in response to meals. It also couples with G proteins to initiate intracellular signaling cascades important for energy homeostasis.
Biological function summary

GPR119 functions as a sensor of lipid-derived molecules often acting as an aryl agonist receptor. It is not part of a larger complex but can activate a series of downstream pathways upon ligand binding. When activated by endogenous and synthetic agonists GPR119 stimulates the release of incretin hormones such as GLP-1 and GIP. This hormone release enhances insulin response and promotes optimal glycemic control essential in maintaining blood glucose levels.

Pathways

GPR119 signaling pathway is integral in glucose metabolism and energy balance. It links to both the incretin hormone signaling pathway and the insulin signaling pathway. Upon activation it influences the levels of cAMP an important secondary messenger that modulates several downstream effects including the regulation of the insulin receptor. GPR119 interacts with other proteins such as adenylate cyclase affecting insulin secretion and lipid metabolism.

GPR119 has significant relevance to type 2 diabetes and obesity. Its role in glucose homeostasis and energy balance makes it a potential therapeutic target for these conditions. Researchers examine GPR119 agonists as possible treatments for enhancing insulin secretion and controlling body weight. The receptor is linked to related pathways integrating multiple metabolic signals including those mediated by GLP-1 an important hormone involved in energy regulation and glycemic control.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the endogenous fatty-acid ethanolamide oleoylethanolamide (OEA) and lysophosphatidylcholine (LPC). Functions as a glucose-dependent insulinotropic receptor. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Seems to act through a G(s) mediated pathway.
See full target information GPR119
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Product promise

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