AB198817

Anti-GPCR GPR124 antibody

Name: Anti-GPCR GPR124 antibody (ab198817) | Abcam
Brand: Abcam Limited
SKU: AB198817
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal GPCR GPR124 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ADGRA2.

View Alternative Names

GPR124, KIAA1531, TEM5, ADGRA2, Adhesion G protein-coupled receptor A2, G-protein coupled receptor 124, Tumor endothelial marker 5

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR124 antibody (AB198817)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR124 antibody (AB198817)

Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling GPCR GPR124 with ab198817 at a 1/40 dilution.

Supplier Data

Western blot - Anti-GPCR GPR124 antibody (AB198817)

Gel : 6% SDS-PAGE

All lanes:

Western blot - Anti-GPCR GPR124 antibody (ab198817) at 1/300 dilution

All lanes:

Mouse bladder tissue lysate at 40 µg

Predicted band size: 143 kDa

false

Exposure time: 40s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ADGRA2. The exact immunogen used to generate this antibody is proprietary information.

Q96PE1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/300", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GPCR GPR124 also known as Tem5 is a member of the G protein-coupled receptor family. This receptor has a molecular mass of approximately 153 kDa. GPR124 localizes mainly in the central nervous system and also shows expression in endothelial cells of blood vessels. This protein functions as a receptor involved in cellular signaling through its interaction with other proteins and extracellular molecules.

Biological function summary

The receptor plays a significant role in vascular development and angiogenesis. GPR124 forms part of a larger protein complex and contributes to maintaining blood-brain barrier integrity. It acts through its ability to bind to ligands influencing cellular processes that are essential for the proper formation and functioning of blood vessels.

Pathways

Interactions in which GPR124 participates have a notable impact on the Wnt signaling pathway. It collaborates with other proteins such as Frizzled receptors and Dishevelled proteins within this pathway. This interaction influences cellular events like proliferation and differentiation important for proper embryonic development and maintenance of adult tissue homeostasis.

Studies link GPR124 to brain vascular malformations and certain types of cancer. Alterations in its expression or function can lead to cerebral cavernous malformations a condition characterized by irregular blood vessel formation in the brain. Additionally GPR124's interplay with VEGFA an important protein in angiogenesis suggests its involvement in tumorigenesis processes that rely on blood vessel growth for tumor survival and expansion.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Endothelial receptor which functions together with RECK to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (PubMed : 28289266, PubMed : 30026314). Plays a key role in Wnt7-specific responses, such as endothelial cell sprouting and migration in the forebrain and neural tube, and establishment of the blood-brain barrier (By similarity). Acts as a Wnt7-specific coactivator of canonical Wnt signaling : required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (PubMed : 30026314). ADGRA2-tethering function does not rely on its G-protein coupled receptor (GPCR) structure but instead on its combined capacity to interact with RECK extracellularly and recruit the Dishevelled scaffolding protein intracellularly (PubMed : 30026314). Binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate (PubMed : 16982628).

See full target information ADGRA2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

AMB Express 9:178 PubMed31673813

2019

Protective and therapeutic effects of Trianthema portulacastrum against atherosclerosis in male albino rats via G-protein-coupled receptor 124.

Applications

Unspecified application

Species

Unspecified reactive species

Haoyu Wu,Tianjiao Gao,Yiwei Cao,Jiayu Diao,Fengjun Chang,Jie Qi,Congxia Wang

The Journal of biological chemistry 294:11920-11933 PubMed31201273

2019

Solution structure and functional investigation of human guanylate kinase reveals allosteric networking and a crucial role for the enzyme in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nazimuddin Khan,Parag P Shah,David Ban,Pablo Trigo-Mouriño,Marta G Carneiro,Lynn DeLeeuw,William L Dean,John O Trent,Levi J Beverly,Manfred Konrad,Donghan Lee,T Michael Sabo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GPCR GPR124 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR124 antibody (AB198817)

Western blot - Anti-GPCR GPR124 antibody (AB198817)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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