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AB51705

Anti-GPCR GPR125 antibody

5

(1 Review)

|

(19 Publications)

Rabbit Polyclonal GPCR GPR125 antibody. Suitable for ELISA, ICC/IF and reacts with Synthetic peptide, Human samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human ADGRA3 aa 300-400 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

GPR125, UNQ556/PRO1113, ADGRA3, Adhesion G protein-coupled receptor A3, G-protein coupled receptor 125

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR125 antibody (AB51705)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR125 antibody (AB51705)

ICC/IF image of ab51705 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51705, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ELISA - Anti-GPCR GPR125 antibody (AB51705)
  • ELISA

Supplier Data

ELISA - Anti-GPCR GPR125 antibody (AB51705)

ELISA using ab51705 at varying antibody concentrations and antigen concentration at 1000 ng/mL.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ELISA, ICC/IF

applications

Immunogen

Synthetic Peptide within Human ADGRA3 aa 300-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q8IWK6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

G protein-coupled receptor 125 also known as GPR125 is a member of the adhesion GPCR family. It has an approximate molecular mass of 100 kDa. GPR125 is involved in signal transduction processes functioning as a receptor that spans the cell membrane. This protein is largely expressed in the testis embryonic tissues and various adult tissues including the central nervous system and heart. Its localization suggests it plays a role in complex signaling processes across development and regular adult function.
Biological function summary

GPR125 acts in cellular adhesion and signaling mechanisms. This receptor is not known to form part of a larger molecular complex but interacts with several cellular components to regulate biological processes. GPR125 influences cell communication and has been implicated in maintaining tissue architecture which is essential for normal cellular functions and development. Through its signaling roles GPR125 impacts cell polarity and migration.

Pathways

GPR125 fits into cell signaling and developmental pathways. It associates with the Wnt signaling pathway which is critical for regulating cell proliferation and polarity. This receptor also displays potential links to pathways involving protein kinases related to cell adhesion and migration. Related proteins like Frizzled receptors in the Wnt pathway interact with GPR125 suggesting their collaboration in various signaling networks.

Mutations or dysregulation of GPR125 have connections to cancer and neurodevelopmental disorders. While its exact functional impact on specific cancers like testicular cancer remains under research GPR125's expression patterns suggest a potential role in tumor development or progression. In neurodevelopmental disorders altered GPR125 signaling could affect neural cell communication and adhesion. The protein Intelectin expressed with GPR125 in neural tissues might also contribute to understanding these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Orphan receptor that may have a role in planar cell polarity pathway.
See full target information ADGRA3
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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 35:102342 PubMed40207021

2025

Erratum: PAK1 Promotes the Proliferation and Inhibits Apoptosis of Human Spermatogonial Stem Cells via PDK1/KDR/ZNF367 and ERK1/2 and AKT Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyong Fu,Wenhui Zhang,Qingqing Yuan,Minghui Niu,Fan Zhou,Qianqian Qiu,Guoping Mao,Hong Wang,Liping Wen,Min Sun,Zheng Li,Zuping He

Advanced healthcare materials 14:e2402606 PubMed39955738

2025

3D Bioprinted Coaxial Testis Model Using Human Induced Pluripotent Stem Cells:A Step Toward Bicompartmental Cytoarchitecture and Functionalization.

Applications

Unspecified application

Species

Unspecified reactive species

Meghan A Robinson,Sonia Hy Kung,Khaled Ym Youssef,Kali M Scheck,Robert H Bell,Funda Sar,Anne M Haegert,M Mahdi Asmae,Changfeng Cheng,Salina V Yeack,Bhairvi T Mathur,Feng Jiang,Colin C Collins,Faraz Hach,Stephanie M Willerth,Ryan K Flannigan

Scientific reports 14:15732 PubMed38977826

2024

The dynamic expression of YAP is essential for the development of male germ cells derived from human embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sujittra Khampang,Chanchao Lorthongpanich,Chuti Laowtammathron,Phatchanat Klaihmon,Sukanya Meesa,Wichuda Suksomboon,Nittaya Jiamvoraphong,Pakpoom Kheolamai,Sudjit Luanpitpong,Charles A Easley,Eisa Mahyari,Surapol Issaragrisil

BMC cancer 21:53 PubMed33430814

2021

Adhesion G protein-coupled receptor, ELTD1, is a potential therapeutic target for retinoblastoma migration and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan Guihurt Santiago,Neikelyn Burgos-Tirado,Daniella Dorta Lafontaine,José C Mendoza Sierra,Roberto Herrera Camacho,Clara M Vecchini Rodríguez,Vanessa Morales-Tirado,Jacqueline Flores-Otero

Molecular therapy. Nucleic acids 20:788-800 PubMed32438314

2020

Hsa-miR-1908-3p Mediates the Self-Renewal and Apoptosis of Human Spermatogonial Stem Cells via Targeting KLF2.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Chen,Yinghong Cui,Bang Liu,Chunyun Li,Li Du,Ruiling Tang,Lulu Qin,Yiqun Jiang,Jian Li,Xing Yu,Quanyuan He,Zuping He

Medical science monitor : international medical journal of experimental and clinical research 24:6608-6616 PubMed30231258

2018

Elevated G-Protein Receptor 125 (GPR125) Expression Predicts Good Outcomes in Colorectal Cancer and Inhibits Wnt/β-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yibin Wu,Wenjie Chen,Lifeng Gong,Chongwei Ke,Huipeng Wang,Yuankun Cai

Cell stem cell 23:599-614.e4 PubMed30174296

2018

Single-Cell RNA Sequencing Analysis Reveals Sequential Cell Fate Transition during Human Spermatogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Wang,Xixi Liu,Gang Chang,Yidong Chen,Geng An,Liying Yan,Shuai Gao,Yanwen Xu,Yueli Cui,Ji Dong,Yuhan Chen,Xiaoying Fan,Yuqiong Hu,Ke Song,Xiaohui Zhu,Yun Gao,Zhaokai Yao,Shuhui Bian,Yu Hou,Jiahao Lu,Rui Wang,Yong Fan,Ying Lian,Wenhao Tang,Yapeng Wang,Jianqiao Liu,Lianming Zhao,Luyu Wang,Zhaoting Liu,Renpei Yuan,Yujia Shi,Boqiang Hu,Xiulian Ren,Fuchou Tang,Xiao-Yang Zhao,Jie Qiao

Molecular therapy. Nucleic acids 12:769-786 PubMed30141410

2018

PAK1 Promotes the Proliferation and Inhibits Apoptosis of Human Spermatogonial Stem Cells via PDK1/KDR/ZNF367 and ERK1/2 and AKT Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyong Fu,Wenhui Zhang,Qingqing Yuan,Minghui Niu,Fan Zhou,Qianqian Qiu,Guoping Mao,Hong Wang,Liping Wen,Min Sun,Zheng Li,Zuping He

Cell death & disease 9:76 PubMed29362488

2018

Transcriptional regulation of P63 on the apoptosis of male germ cells and three stages of spermatogenesis in mice.

Applications

ICC/IF

Species

Unspecified reactive species

Hong Wang,Qingqing Yuan,Minghui Niu,Wenhui Zhang,Liping Wen,Hongyong Fu,Fan Zhou,Zuping He

Cell cycle (Georgetown, Tex.) 17:225-239 PubMed29169284

2018

VEGFC/VEGFR3 Signaling Regulates Mouse Spermatogonial Cell Proliferation via the Activation of AKT/MAPK and Cyclin D1 Pathway and Mediates the Apoptosis by affecting Caspase 3/9 and Bcl-2.

Applications

Unspecified application

Species

Unspecified reactive species

Liangyu Zhao,Zijue Zhu,Chencheng Yao,Yuhua Huang,Erlei Zhi,Huixing Chen,Ruhui Tian,Peng Li,Qingqing Yuan,Yunjing Xue,Zhong Wan,Chao Yang,Yuehua Gong,Zuping He,Zheng Li
View all publications
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