Rabbit Polyclonal GPCR GPR125 antibody. Suitable for ELISA, ICC/IF and reacts with Synthetic peptide, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human ADGRA3 aa 300-400 conjugated to Keyhole Limpet Haemocyanin.
Images
Publications
Filter by
- BMC cancer 21:532021Adhesion G protein-coupled receptor, ELTD1, is a potential therapeutic target for retinoblastoma migration and invasion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonathan Guihurt Santiago et. al.PubMed 33430814
- Molecular therapy. Nucleic acids 20:788-8002020Hsa-miR-1908-3p Mediates the Self-Renewal and Apoptosis of Human Spermatogonial Stem Cells via Targeting KLF2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Chen et. al.PubMed 32438314
- Medical science monitor : international medical journal of experimental and clinical research 24:6608-66162018Elevated G-Protein Receptor 125 (GPR125) Expression Predicts Good Outcomes in Colorectal Cancer and Inhibits Wnt/β-Catenin Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYibin Wu,Wenjie Chen,Lifeng Gong,Chongwei Ke,Huipeng Wang,Yuankun CaiPubMed 30231258
- Molecular therapy. Nucleic acids 12:769-7862018PAK1 Promotes the Proliferation and Inhibits Apoptosis of Human Spermatogonial Stem Cells via PDK1/KDR/ZNF367 and ERK1/2 and AKT Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongyong Fu et. al.PubMed 30141410
- Cell death & disease 9:762018Transcriptional regulation of P63 on the apoptosis of male germ cells and three stages of spermatogenesis in mice.
- Cell cycle (Georgetown, Tex.) 17:225-2392018VEGFC/VEGFR3 Signaling Regulates Mouse Spermatogonial Cell Proliferation via the Activation of AKT/MAPK and Cyclin D1 Pathway and Mediates the Apoptosis by affecting Caspase 3/9 and Bcl-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiangyu Zhao et. al.PubMed 29169284
- Reproduction in domestic animals = Zuchthygiene 52 Suppl 2:177-1862017Molecular markers of putative spermatogonial stem cells in the domestic cat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS J Bedford-Guaus,S Kim,L Mulero,J M Vaquero,C Morera,R Adan-Milanès,A Veiga,Á RayaPubMed 28402059
- Stem cell research & therapy 8:372017In vitro generation of Sertoli-like and haploid spermatid-like cells from human umbilical cord perivascular cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEkaterina Shlush et. al.PubMed 28202061
- Reproduction (Cambridge, England) :2016Human umbilical perivascular cells: a novel source of MSCs to support testicular niche regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeila Maghen et. al.PubMed 27780883
- Reproduction (Cambridge, England) 148:417-272014Characterization of human spermatogonial stem cell markers in fetal, pediatric, and adult testicular tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEran Altman,Pamela Yango,Radwa Moustafa,James F Smith,Peter C Klatsky,Nam D TranPubMed 25030892
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: Whole serum
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ADGRA3 aa 300-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IWK6
Reactivity data
Select an application| ELISA | ICC/IF | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Predicted | Predicted |
| Synthetic peptide | Tested | Not recommended |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Target data
Function
Orphan receptor that may have a role in planar cell polarity pathway.
Alternative names
GPR125, UNQ556/PRO1113, ADGRA3, Adhesion G protein-coupled receptor A3, G-protein coupled receptor 125
Recommended products
Rabbit Polyclonal GPCR GPR125 antibody. Suitable for ELISA, ICC/IF and reacts with Synthetic peptide, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human ADGRA3 aa 300-400 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: Whole serum
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ADGRA3 aa 300-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IWK6
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
G protein-coupled receptor 125 also known as GPR125 is a member of the adhesion GPCR family. It has an approximate molecular mass of 100 kDa. GPR125 is involved in signal transduction processes functioning as a receptor that spans the cell membrane. This protein is largely expressed in the testis embryonic tissues and various adult tissues including the central nervous system and heart. Its localization suggests it plays a role in complex signaling processes across development and regular adult function.
- Biological function summary
GPR125 acts in cellular adhesion and signaling mechanisms. This receptor is not known to form part of a larger molecular complex but interacts with several cellular components to regulate biological processes. GPR125 influences cell communication and has been implicated in maintaining tissue architecture which is essential for normal cellular functions and development. Through its signaling roles GPR125 impacts cell polarity and migration.
- Pathways
GPR125 fits into cell signaling and developmental pathways. It associates with the Wnt signaling pathway which is critical for regulating cell proliferation and polarity. This receptor also displays potential links to pathways involving protein kinases related to cell adhesion and migration. Related proteins like Frizzled receptors in the Wnt pathway interact with GPR125 suggesting their collaboration in various signaling networks.
- Associated diseases and disorders
Mutations or dysregulation of GPR125 have connections to cancer and neurodevelopmental disorders. While its exact functional impact on specific cancers like testicular cancer remains under research GPR125's expression patterns suggest a potential role in tumor development or progression. In neurodevelopmental disorders altered GPR125 signaling could affect neural cell communication and adhesion. The protein Intelectin expressed with GPR125 in neural tissues might also contribute to understanding these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR125 antibody (ab51705)
ICC/IF image of ab51705 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51705, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ELISA - Anti-GPCR GPR125 antibody (ab51705)
ELISA using ab51705 at varying antibody concentrations and antigen concentration at 1000 ng/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com