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AB8104

Anti-GPCR GPR15 antibody

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(1 Review)

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(3 Publications)

Rabbit Polyclonal GPCR GPR15 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human GPR15 aa 1-50.

View Alternative Names

G-protein coupled receptor 15, Brother of Bonzo, BoB, GPR15

2 Images
Western blot - Anti-GPCR GPR15 antibody (AB8104)
  • WB

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Western blot - Anti-GPCR GPR15 antibody (AB8104)

Western blot analysis of GPR15 in human heart lysate with anti- GPR15 (NT) in the absence (lane 1) or presence of specific peptide (lane 2) or non related peptide (lane 3).

All lanes:

Western blot - Anti-GPCR GPR15 antibody (ab8104)

Lane 1:

Human heart lysate in absence of specific peptide

Lane 2:

Human heart lysate in presence of specific peptide

Lane 3:

Human heart lysate in presence of unrelated peptide

Predicted band size: 41 kDa

Observed band size: 50 kDa

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Western blot - Anti-GPCR GPR15 antibody (AB8104)
  • WB

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Western blot - Anti-GPCR GPR15 antibody (AB8104)

Lane 1:

Western blot - Anti-GPCR GPR15 antibody (ab8104) at 0.5 µg/mL

Lane 2:

Western blot - Anti-GPCR GPR15 antibody (ab8104) at 1 µg/mL

All lanes:

Human spleen lysate

Predicted band size: 41 kDa

Observed band size: 50 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human GPR15 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P49685

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Product details

Human immunodeficiency virus (HIV) and related viruses require co-receptors to infect target cells. Some G protein coupled receptors including CCR5, CXCR4, CCR3,CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. The genes encoding human and monkey GPR15/BOB (for G protein-coupled receptor 15 and brother of Bonzo, respectively) were recently cloned. This novel G protein-coupled receptor serves as coreceptor for simian immunodeficiency virus (SIV), and for strains of HIV-2 and M-tropic HIV-1. The ligand for GPR15 has not been identified yet.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The G-protein coupled receptor GPR15 also known as BOB is a seven-transmembrane domain receptor belonging to the GPCR superfamily. While its exact molecular mass varies slightly between species it generally hovers around 40–45 kDa. GPR15 is mainly expressed in the gastrointestinal tract particularly the colon as well as in immune cells like T-cells. The receptor is involved in binding and signal transduction which triggers downstream signaling pathways. Researchers often use GPCR antibodies to study interactions involving GPR15 which holds significance in understanding its mechanical functionality.
Biological function summary

GPR15 functions as a mediator for immune and inflammatory responses. It plays a role in T-cell trafficking to mucosal tissues where it helps manage immune surveillance and response. Its biological function does not seem to involve participation in large protein complexes. The receptor interacts with its ligand GPR15L facilitating signaling cascades that impact immune cell behavior. This interaction is essential for processes like maintaining intestinal homeostasis and could have broader implications in immune system regulation.

Pathways

GPR15 is closely associated with the chemokine-mediated signaling pathways. It modulates the migration and adhesion of immune cells which are critical for maintaining mucosal immunity. GPR15L serves as its ligand in these pathways which highlights their regulatory role in immune responses. GPR15 also intersects with inflammatory cytokine signaling highlighting its involvement in pathways that direct inflammatory responses and immune cell homeostasis in the gut environment.

GPR15 has been linked notably to conditions such as inflammatory bowel disease (IBD) and certain types of cancer particularly colorectal cancer. In IBD GPR15-mediated cell migration can exacerbate inflammatory responses in the intestinal lining. The receptor's overexpression has been observed in colorectal cancer potentially contributing to tumor progression through similar immune modulation pathways. By regulating immune cell traffic GPR15 connects with other proteins involved in disease pathways potentially offering targets for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable chemokine receptor. Alternative coreceptor with CD4 for HIV-1 infection.
See full target information GPR15
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Anticancer research 42:4679-4687 PubMed36191971

2022

Recombinant Thrombomodulin Has Anti-tumor Effects and Enhances the Effects of Gemcitabine for Pancreatic Cancer Through G-protein Coupled Receptor 15.

Applications

Unspecified application

Species

Unspecified reactive species

Kenei Furukawa,Jianhua Ling,Yichen Sun,Y U Lu,Jie Fu,Rui Marukuchi,Paul J Chiao

Scientific reports 7:692 PubMed28386128

2017

G-protein coupled receptor 15 mediates angiogenesis and cytoprotective function of thrombomodulin.

Applications

WB

Species

Unspecified reactive species

Bin Pan,Xiangmin Wang,Chie Nishioka,Goichi Honda,Akihito Yokoyama,Lingyu Zeng,Kailin Xu,Takayuki Ikezoe

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 23:746-756 PubMed28167153

2017

The Fifth Epidermal Growth Factor-like Region of Thrombomodulin Alleviates Murine Graft-versus-Host Disease in a G-Protein Coupled Receptor 15 Dependent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Pan,Xiangmin Wang,Shinsuke Kojima,Chie Nishioka,Akihito Yokoyama,Goichi Honda,Kailin Xu,Takayuki Ikezoe
View all publications
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