AB106781

Anti-GPCR GPR17 antibody

Name: Anti-GPCR GPR17 antibody (ab106781) | Abcam
Brand: Abcam Limited
SKU: AB106781
Price: 535 USD
Availability: InStock

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(3 Publications)

Goat Polyclonal GPCR GPR17 antibody. Suitable for ICC, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human GPR17 aa 350 to C-terminus.

View Alternative Names

Uracil nucleotide/cysteinyl leukotriene receptor, UDP/CysLT receptor, G-protein coupled receptor 17, P2Y-like receptor, R12, GPR17

3 Images

Supplier Data

Immunocytochemistry - Anti-GPCR GPR17 antibody (AB106781)

ab106781 staining GPCR GPR17 in U251 (Human brain glioma cell line) cells by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton. Samples were incubated with primary antibody (10 ug/ml) for 1 hour, followed by incubation by an Alexa Fluor^® 488 secondary antibody (2 ug/ml).

Negative control : Unimmunized goat IgG (10 ug/ml), followed by an Alexa Fluor^® 488 secondary antibody (2 ug/ml).

Supplier Data

Immunocytochemistry - Anti-GPCR GPR17 antibody (AB106781)

ab106781 staining GPCR GPR17 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton. Samples were incubated with primary antibody (10 ug/ml) for 1 hour, followed by incubation by an Alexa Fluor^® 488 secondary antibody (2 ug/ml).

Negative control : Unimmunized goat IgG (10 ug/ml), followed by an Alexa Fluor^® 488 secondary antibody (2 ug/ml).

Supplier Data

Western blot - Anti-GPCR GPR17 antibody (AB106781)

Western blot analysis of mouse brain cell lysate labeling GPCR GPR17 with ab106781 at 3 ug/ml.

All lanes:

Western blot - Anti-GPCR GPR17 antibody (ab106781) at 3 µg/mL

All lanes:

Mouse brain cell lysate

Predicted band size: 41 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC

applications

Immunogen

Synthetic Peptide within Human GPR17 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13304

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPR17 also known as G protein-coupled receptor 17 is a member of the G protein-coupled receptor (GPCR) family. This receptor has a molecular weight of approximately 40 kDa. It is expressed in various tissues with significant levels found in the central nervous system including the brain and spinal cord. GPR17 also shows expression in kidneys liver and heart. It is classified under the class A Rhodopsin-like family and is an orphan receptor meaning its natural ligand is not completely established but is thought to be associated with uridine nucleotides and cysteinyl leukotrienes.

Biological function summary

GPR17 is involved in several physiological processes particularly in the regulation of inflammation and myelination within the central nervous system. It plays a critical role in the differentiation of oligodendrocyte precursor cells into mature oligodendrocytes which are essential for myelin sheath formation and repair of neural damage. GPR17 is not known to function as part of a larger receptor complex but it interacts with intracellular signaling pathways to modulate these cellular processes.

Pathways

GPR17 integrates into inflammatory response pathways and neural cell development pathways. It is actively involved in the nucleotide signaling pathway which is important for responses to tissue damage and initiating repair processes. GPR17 shares functional connections with proteins such as P2Y receptors which also respond to nucleotides and are involved in diverse cellular responses including muscle contraction and platelet aggregation.

GPR17's expression and role connect it to multiple sclerosis and brain ischemia. In multiple sclerosis dysregulation of GPR17 can affect the formation and maintenance of the myelin sheath contributing to neural degeneration. During brain ischemia GPR17 activation influences the extent of injury and subsequent recovery through its effects on inflammation and tissue repair. It is linked to proteins like CXCR7 which similarly influence neural damage and repair processes in ischemic conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Dual specificity receptor for uracil nucleotides and cysteinyl leukotrienes (CysLTs). Signals through G(i) and inhibition of adenylyl cyclase. May mediate brain damage by nucleotides and CysLTs following ischemia.

See full target information GPR17

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International immunopharmacology 88:106870 PubMed32805694

2020

Inhibition of GPR17 with pranlukast protects against TNF-α-induced loss of type II collagen in ATDC5 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhangfu Wang,Weiwei Zhou,Guangbin Zheng,Guangyong Yang

Cancers 12: PubMed32466580

2020

ESM-1 Overexpression is Involved in Increased Tumorigenesis of Radiotherapy-Resistant Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hana Jin,Trojan Rugira,Young Shin Ko,Sang Won Park,Seung Pil Yun,Hye Jung Kim

Development (Cambridge, England) 146: PubMed31444216

2019

GPR17 is an essential regulator for the temporal adaptation of sonic hedgehog signalling in neural tube development.

Applications

Unspecified application

Species

Unspecified reactive species

Atsuki Yatsuzuka,Akiko Hori,Minori Kadoya,Mami Matsuo-Takasaki,Toru Kondo,Noriaki Sasai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GPCR GPR17 antibody

Immunocytochemistry - Anti-GPCR GPR17 antibody (AB106781)

Immunocytochemistry - Anti-GPCR GPR17 antibody (AB106781)

Western blot - Anti-GPCR GPR17 antibody (AB106781)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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