Anti-GPCR GPR17 antibody [EPR26422-118]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling GPCR GPR17 with ab314307 at 1/500 (1.034 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human liver. The section was incubated with ab314307 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling GPCR GPR17 with ab314307 at 1/500 (1.034 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human skeletal muscle. The section was incubated with ab314307 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling GPCR GPR17 with ab314307 at 1/500 (1.034 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on oligodendrocytes of human cerebrum. The section was incubated with ab314307 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Immunohistochemical analysis of paraffin-embedded human spinal cord tissue labeling GPCR GPR17 with ab314307 at 1/500 (1.034 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on oligodendrocytes of human spinal cord. The section was incubated with ab314307 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

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Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human cerebrum tissue section labelling Noradrenaline transporter with ab254361 at a 1/100 dilution (B), GPCR GPR17 with ab314307 at a 1/500 dilution (C), and ARMET/ARP with ab316935 at a 1/1000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on human cerebrum.
Panel B : anti-Noradrenaline transporter staining nerves in human cerebrum.
Panel C : anti-GPCR GPR17 staining oligodendrocytes in human cerebrum.
Panel D : anti-ARMET/ARP staining neurons in human cerebrum.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The section was incubated in three rounds of staining : in the order of ab254361, ab314307 and ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

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Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Fluorescence multiplex immunohistochemical analysis of the human cerebrum (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-GFAP (magenta; Opal™690), anti-MAP2 (green; Opal™520) and anti-GPCR GPR17 (red; Opal™570) on human cerebrum. Panel B : anti-MAP2 staining neurons. Panel C : anti-GPCR GPR17 staining oligodendrocytes. Panel D : anti-GFAP staining astrocytes. The section was incubated in three rounds of staining : in the order of ab254263, ab314307, and ab68428 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IP

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Immunoprecipitation - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

GPCR GPR17 was immunoprecipitated from 0.35 mg human spinal cord tissue lysate with ab314307 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314307 at 1/1000 dilution.

VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Human spinal cord tissue lysate

Lane 2 : ab314307 IP in Human spinal cord tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314307 in human spinal cord tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Immunoprecipitation - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307) at 1/30 dilution

All lanes:

Human spinal cord tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

true

Exposure time: 180s

• mIHC

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Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum labelling Noradrenaline transporter with ab254361 at 1/100 (B), GPCR GPR17 with ab314307 at 1/2000 dilution (C) and FMRP with ab259335 at 1/10000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™690), anti-GPCR GPR17 (green; Opal™520) and anti-FMRP (gray; Opal™570) on rat cerebrum.
Panel B : anti-Noradrenaline transporter staining nerves in rat cerebrum.
Panel C : anti-GPCR GPR17 staining oligodendrocytes in rat cerebrum.
Panel D : anti-FMRP staining neurons in rat cerebrum.

The section was incubated in three rounds of staining : in the order of ab254361, ab314307 and ab259335 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling GPCR GPR17 with ab314307 at 1/100 (5.17 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in partial mouse primary neural/glia cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling GPCR GPR17 with ab314307 at 1/100 (5.17 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in partial rat primary neural/glia cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• mIHC

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Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat spinal cord tissue section labelling GPCR GPR17 with ab314307 at 1/2000 dilution (B), TAC1 with ab307138 at 1/500 dilution (C), and ARMET/ARP with ab316935 at 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-GPCR GPR17 (magenta; Opal™ 690), anti-TAC1 (green; Opal™ 520) and anti-ARMET/ARP (yellow; Opal™ 570) on rat spinal cord.
Panel B : anti-GPCR GPR17 staining oligodendrocytes in rat spinal cord.
Panel C : anti-TAC1 staining posterior horns (sensory) in rat spinal cord.
Panel D : anti-ARMET/ARP staining neurons in rat spinal cord.

The section was incubated in three rounds of staining : in the order of ab314307, ab307138 and ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spinal cord tissue section labelling GPCR GPR17 with ab314307 at 1/2000 dilution (B), TAC1 with ab307138 at 1/500 dilution (C), and ARMET/ARP with ab316935 at 1/20000 (D). Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-GPCR GPR17 (magenta; Opal™ 690), anti-TAC1 (green; Opal™ 520) and anti-ARMET/ARP (yellow; Opal™ 570) on mouse spinal cord.
Panel B : anti-GPCR GPR17 staining oligodendrocytes in mouse spinal cord.
Panel C : anti-TAC1 staining posterior horns (sensory) in mouse spinal cord.
Panel D : anti-ARMET/ARP staining neurons in mouse spinal cord.

The section was incubated in three rounds of staining : in the order of ab314307, ab307138 and ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebrum tissue section labelling Noradrenaline transporter with ab254361 at a 1/100 dilution (B), GPCR GPR17 with ab314307 at a 1/2000 dilution (C), and ARMET/ARP with ab316935 at a 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on mouse cerebrum.
Panel B : anti-Noradrenaline transporter staining nerves in mouse cerebrum.
Panel C : anti-GPCR GPR17 staining oligodendrocytes in mouse cerebrum.
Panel D : anti-ARMET/ARP staining neurons in mouse cerebrum.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The section was incubated in three rounds of staining : in the order of ab254361, ab314307 and ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat cerebellum tissue section labelling Noradrenaline transporter with ab254361 at a 1/100 dilution (B), GPCR GPR17 with ab314307 at a 1/2000 dilution (C), and ARMET/ARP with ab316935 at a 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on rat cerebellum.
Panel B : anti-Noradrenaline transporter staining nerves in rat cerebellum.
Panel C : anti-GPCR GPR17 staining oligodendrocytes in rat cerebellum.
Panel D : anti-ARMET/ARP staining neurons in rat cerebellum.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The section was incubated in three rounds of staining : in the order of ab254361, ab314307 and ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat cerebrum tissue section labelling Noradrenaline transporter with ab254361 at a 1/100 dilution (B), GPCR GPR17 with ab314307 at a 1/2000 dilution (C), and ARMET/ARP with ab316935 at a 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on rat cerebrum.
Panel B : anti-Noradrenaline transporter staining nerves in rat cerebrum.
Panel C : anti-GPCR GPR17 staining oligodendrocytes in rat cerebrum.
Panel D : anti-ARMET/ARP staining neurons in rat cerebrum.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The section was incubated in three rounds of staining : in the order of ab254361, ab314307 and ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling GPCR GPR17 with ab314307 at 1/2000 (0.259 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse liver. The section was incubated with ab314307 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebellum tissue section labelling Noradrenaline transporter with ab254361 at a 1/100 dilution (B), GPCR GPR17 with ab314307 at a 1/2000 dilution (C), and ARMET/ARP with ab316935 at a 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on mouse cerebellum.
Panel B : anti-Noradrenaline transporter staining nerves in mouse cerebellum.
Panel C : anti-GPCR GPR17 staining oligodendrocytes in mouse cerebellum.
Panel D : anti-ARMET/ARP staining neurons in mouse cerebellum.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The section was incubated in three rounds of staining : in the order of ab254361, ab314307 and ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling GPCR GPR17 with ab314307 at 1/2000 (0.259 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on rat liver. The section was incubated with ab314307 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling GPCR GPR17 with ab314307 at 1/2000 (0.259 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on oligodendrocytes of mouse cerebrum. The section was incubated with ab314307 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling GPCR GPR17 with ab314307 at 1/2000 (0.259 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on oligodendrocytes of rat cerebrum. The section was incubated with ab314307 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebellum labelling Noradrenaline transporter with ab254361 at 1/100 (B), GPCR GPR17 with ab314307 at 1/2000 dilution (C) and FMRP with ab259335 at 1/10000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™690), anti-GPCR GPR17 (green; Opal™520) and anti-FMRP (gray; Opal™570) on mouse cerebellum.

Panel B : anti-Noradrenaline transporter staining nerves in mouse cerebellum.

Panel C : anti-GPCR GPR17 staining oligodendrocytes in mouse cerebellum.

Panel D : anti-FMRP staining neurons in mouse cerebellum.

The section was incubated in three rounds of staining : in the order of ab254361, ab314307 and ab259335 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum labelling Noradrenaline transporter with ab254361 at 1/100 (B), GPCR GPR17 with ab314307 at 1/2000 dilution (C) and FMRP with ab259335 at 1/10000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™690), anti-GPCR GPR17 (green; Opal™520) and anti-FMRP (gray; Opal™570) on mouse cerebrum.

Panel B : anti-Noradrenaline transporter staining nerves in mouse cerebrum.

Panel C : anti-GPCR GPR17 staining oligodendrocytes in mouse cerebrum.

Panel D : anti-FMRP staining neurons in mouse cerebrum.

The section was incubated in three rounds of staining : in the order of ab254361, ab314307 and ab259335 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellum labelling Noradrenaline transporter with ab254361 at 1/100 (B), GPCR GPR17 with ab314307 at 1/2000 dilution (C) and FMRP with ab259335 at 1/10000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™690), anti-GPCR GPR17 (green; Opal™520) and anti-FMRP (gray; Opal™570) on rat cerebellum.

Panel B : anti-Noradrenaline transporter staining nerves in rat cerebellum.

Panel C : anti-GPCR GPR17 staining oligodendrocytes in rat cerebellum.

Panel D : anti-FMRP staining neurons in rat cerebellum.

The section was incubated in three rounds of staining : in the order of ab254361, ab314307 and ab259335 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.