Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Rabbit Recombinant Monoclonal GPCR GPR17 antibody. Suitable for IHC-P, IP, ICC/IF, mIHC and reacts with Human, Mouse, Rat samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR17 antibody [EPR26422-118] (AB314307), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	ICC/IF	mIHC	WB	Flow Cyt
Human	Tested	Tested	Expected	Tested	Not recommended	Not recommended
Mouse	Tested	Expected	Tested	Tested	Not recommended	Not recommended
Rat	Tested	Expected	Tested	Tested	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -
Species Rat	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500 - 1/20000	Notes -
Species Mouse	Dilution info 1/500 - 1/20000	Notes -
Species Rat	Dilution info 1/500 - 1/20000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Associated Products

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Target data

See full target information GPR17

Function

Dual specificity receptor for uracil nucleotides and cysteinyl leukotrienes (CysLTs). Signals through G(i) and inhibition of adenylyl cyclase. May mediate brain damage by nucleotides and CysLTs following ischemia.

Alternative names

Uracil nucleotide/cysteinyl leukotriene receptor, UDP/CysLT receptor, G-protein coupled receptor 17, P2Y-like receptor, R12, GPR17

Recommended products

Rabbit Recombinant Monoclonal GPCR GPR17 antibody. Suitable for IHC-P, IP, ICC/IF, mIHC and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR26422-118
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GPR17 also known as G protein-coupled receptor 17 is a member of the G protein-coupled receptor (GPCR) family. This receptor has a molecular weight of approximately 40 kDa. It is expressed in various tissues with significant levels found in the central nervous system including the brain and spinal cord. GPR17 also shows expression in kidneys liver and heart. It is classified under the class A Rhodopsin-like family and is an orphan receptor meaning its natural ligand is not completely established but is thought to be associated with uridine nucleotides and cysteinyl leukotrienes.

Biological function summary

GPR17 is involved in several physiological processes particularly in the regulation of inflammation and myelination within the central nervous system. It plays a critical role in the differentiation of oligodendrocyte precursor cells into mature oligodendrocytes which are essential for myelin sheath formation and repair of neural damage. GPR17 is not known to function as part of a larger receptor complex but it interacts with intracellular signaling pathways to modulate these cellular processes.

Pathways

GPR17 integrates into inflammatory response pathways and neural cell development pathways. It is actively involved in the nucleotide signaling pathway which is important for responses to tissue damage and initiating repair processes. GPR17 shares functional connections with proteins such as P2Y receptors which also respond to nucleotides and are involved in diverse cellular responses including muscle contraction and platelet aggregation.

Associated diseases and disorders

GPR17's expression and role connect it to multiple sclerosis and brain ischemia. In multiple sclerosis dysregulation of GPR17 can affect the formation and maintenance of the myelin sheath contributing to neural degeneration. During brain ischemia GPR17 activation influences the extent of injury and subsequent recovery through its effects on inflammation and tissue repair. It is linked to proteins like CXCR7 which similarly influence neural damage and repair processes in ischemic conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

23 product images

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
GPCR GPR17 Immunocytochemistry/ Immunofluorescence staining of mouse primary neural/glia cell using rabbit Anti-GPCR GPR17 antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling GPCR GPR17 with ab314307 at 1/100 (5.17 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in partial mouse primary neural/glia cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
GPCR GPR17 Immunocytochemistry/ Immunofluorescence staining of rat primary neural/glia cell using rabbit Anti-GPCR GPR17 antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling GPCR GPR17 with ab314307 at 1/100 (5.17 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in partial rat primary neural/glia cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
GPCR GPR17 Multiplex immunohistochemistry staining of Human cerebrum using rabbit Anti-GPCR GPR17 antibody
Fluorescence multiplex immunohistochemical analysis of the human cerebrum (Formalin/PFA-fixed paraffin-embedded sections).

Panel A: merged staining of anti-GFAP (magenta; Opal™690), anti-MAP2 (green; Opal™520) and anti-GPCR GPR17 (red; Opal™570) on human cerebrum.
Panel B: anti-MAP2 staining neurons.
Panel C: anti-GPCR GPR17 staining oligodendrocytes.
Panel D: anti-GFAP staining astrocytes.

The section was incubated in three rounds of staining: in the order of Anti-MAP2 antibody [EPR22641-106] - Neuronal Marker ab254263, ab314307, and Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab68428 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat spinal cord tissue section labelling GPCR GPR17 with ab314307 at 1/2000 dilution (B), TAC1 with Anti-TAC1 antibody [EPR26567-18-1-3] ab307138 at 1/500 dilution (C), and ARMET/ARP with Anti-ARMET/ARP antibody [EPR29115-74] ab316935 at 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-GPCR GPR17 (magenta; Opal™ 690), anti-TAC1 (green; Opal™ 520) and anti-ARMET/ARP (yellow; Opal™ 570) on rat spinal cord.

Panel B: anti-GPCR GPR17 staining oligodendrocytes in rat spinal cord.

Panel C: anti-TAC1 staining posterior horns (sensory) in rat spinal cord.

Panel D: anti-ARMET/ARP staining neurons in rat spinal cord.
The section was incubated in three rounds of staining: in the order of ab314307, Anti-TAC1 antibody [EPR26567-18-1-3] ab307138 and Anti-ARMET/ARP antibody [EPR29115-74] ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spinal cord tissue section labelling GPCR GPR17 with ab314307 at 1/2000 dilution (B), TAC1 with Anti-TAC1 antibody [EPR26567-18-1-3] ab307138 at 1/500 dilution (C), and ARMET/ARP with Anti-ARMET/ARP antibody [EPR29115-74] ab316935 at 1/20000 (D). Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-GPCR GPR17 (magenta; Opal™ 690), anti-TAC1 (green; Opal™ 520) and anti-ARMET/ARP (yellow; Opal™ 570) on mouse spinal cord.

Panel B: anti-GPCR GPR17 staining oligodendrocytes in mouse spinal cord.

Panel C: anti-TAC1 staining posterior horns (sensory) in mouse spinal cord.

Panel D: anti-ARMET/ARP staining neurons in mouse spinal cord.
The section was incubated in three rounds of staining: in the order of ab314307, Anti-TAC1 antibody [EPR26567-18-1-3] ab307138 and Anti-ARMET/ARP antibody [EPR29115-74] ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Immunoprecipitation - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
GPCR GPR17 was immunoprecipitated from 0.35 mg human spinal cord tissue lysate with ab314307 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314307 at 1/1000 dilution.
VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Human spinal cord tissue lysate
Lane 2: ab314307 IP in Human spinal cord tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314307 in human spinal cord tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Immunoprecipitation - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307) at 1/30 dilution
All lanes: Human spinal cord tissue lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Developed using the ECL technique.
Exposure time: 180s
Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat cerebrum tissue section labelling Noradrenaline transporter with Anti-Noradrenaline transporter antibody [EPR23527-534] ab254361 at a 1/100 dilution (B), GPCR GPR17 with ab314307 at a 1/2000 dilution (C), and ARMET/ARP with Anti-ARMET/ARP antibody [EPR29115-74] ab316935 at a 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A: merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on rat cerebrum.

Panel B: anti-Noradrenaline transporter staining nerves in rat cerebrum.

Panel C: anti-GPCR GPR17 staining oligodendrocytes in rat cerebrum.

Panel D: anti-ARMET/ARP staining neurons in rat cerebrum.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
The section was incubated in three rounds of staining: in the order of Anti-Noradrenaline transporter antibody [EPR23527-534] ab254361, ab314307 and Anti-ARMET/ARP antibody [EPR29115-74] ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human cerebrum tissue section labelling Noradrenaline transporter with Anti-Noradrenaline transporter antibody [EPR23527-534] ab254361 at a 1/100 dilution (B), GPCR GPR17 with ab314307 at a 1/500 dilution (C), and ARMET/ARP with Anti-ARMET/ARP antibody [EPR29115-74] ab316935 at a 1/1000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A: merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on human cerebrum.

Panel B: anti-Noradrenaline transporter staining nerves in human cerebrum.

Panel C: anti-GPCR GPR17 staining oligodendrocytes in human cerebrum.

Panel D: anti-ARMET/ARP staining neurons in human cerebrum.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
The section was incubated in three rounds of staining: in the order of Anti-Noradrenaline transporter antibody [EPR23527-534] ab254361, ab314307 and Anti-ARMET/ARP antibody [EPR29115-74] ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebrum tissue section labelling Noradrenaline transporter with Anti-Noradrenaline transporter antibody [EPR23527-534] ab254361 at a 1/100 dilution (B), GPCR GPR17 with ab314307 at a 1/2000 dilution (C), and ARMET/ARP with Anti-ARMET/ARP antibody [EPR29115-74] ab316935 at a 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A: merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on mouse cerebrum.

Panel B: anti-Noradrenaline transporter staining nerves in mouse cerebrum.

Panel C: anti-GPCR GPR17 staining oligodendrocytes in mouse cerebrum.

Panel D: anti-ARMET/ARP staining neurons in mouse cerebrum.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
The section was incubated in three rounds of staining: in the order of Anti-Noradrenaline transporter antibody [EPR23527-534] ab254361, ab314307 and Anti-ARMET/ARP antibody [EPR29115-74] ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat cerebellum tissue section labelling Noradrenaline transporter with Anti-Noradrenaline transporter antibody [EPR23527-534] ab254361 at a 1/100 dilution (B), GPCR GPR17 with ab314307 at a 1/2000 dilution (C), and ARMET/ARP with Anti-ARMET/ARP antibody [EPR29115-74] ab316935 at a 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A: merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on rat cerebellum.

Panel B: anti-Noradrenaline transporter staining nerves in rat cerebellum.

Panel C: anti-GPCR GPR17 staining oligodendrocytes in rat cerebellum.

Panel D: anti-ARMET/ARP staining neurons in rat cerebellum.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
The section was incubated in three rounds of staining: in the order of Anti-Noradrenaline transporter antibody [EPR23527-534] ab254361, ab314307 and Anti-ARMET/ARP antibody [EPR29115-74] ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebellum tissue section labelling Noradrenaline transporter with Anti-Noradrenaline transporter antibody [EPR23527-534] ab254361 at a 1/100 dilution (B), GPCR GPR17 with ab314307 at a 1/2000 dilution (C), and ARMET/ARP with Anti-ARMET/ARP antibody [EPR29115-74] ab316935 at a 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A: merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on mouse cerebellum.

Panel B: anti-Noradrenaline transporter staining nerves in mouse cerebellum.

Panel C: anti-GPCR GPR17 staining oligodendrocytes in mouse cerebellum.

Panel D: anti-ARMET/ARP staining neurons in mouse cerebellum.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
The section was incubated in three rounds of staining: in the order of Anti-Noradrenaline transporter antibody [EPR23527-534] ab254361, ab314307 and Anti-ARMET/ARP antibody [EPR29115-74] ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebellum labelling Noradrenaline transporter with Anti-Noradrenaline transporter antibody [EPR23527-534] ab254361 at 1/100 (B), GPCR GPR17 with ab314307 at 1/2000 dilution (C) and FMRP with Anti-FMRP antibody [EPR23852-90] ab259335 at 1/10000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A: merged staining of anti-Noradrenaline transporter (magenta; Opal™690), anti-GPCR GPR17 (green; Opal™520) and anti-FMRP (gray; Opal™570) on mouse cerebellum.

Panel B: anti-Noradrenaline transporter staining nerves in mouse cerebellum.

Panel C: anti-GPCR GPR17 staining oligodendrocytes in mouse cerebellum.

Panel D: anti-FMRP staining neurons in mouse cerebellum.

The section was incubated in three rounds of staining: in the order of Anti-Noradrenaline transporter antibody [EPR23527-534] ab254361, ab314307 and Anti-FMRP antibody [EPR23852-90] ab259335 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellum labelling Noradrenaline transporter with Anti-Noradrenaline transporter antibody [EPR23527-534] ab254361 at 1/100 (B), GPCR GPR17 with ab314307 at 1/2000 dilution (C) and FMRP with Anti-FMRP antibody [EPR23852-90] ab259335 at 1/10000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A: merged staining of anti-Noradrenaline transporter (magenta; Opal™690), anti-GPCR GPR17 (green; Opal™520) and anti-FMRP (gray; Opal™570) on rat cerebellum.

Panel B: anti-Noradrenaline transporter staining nerves in rat cerebellum.

Panel C: anti-GPCR GPR17 staining oligodendrocytes in rat cerebellum.

Panel D: anti-FMRP staining neurons in rat cerebellum.

The section was incubated in three rounds of staining: in the order of Anti-Noradrenaline transporter antibody [EPR23527-534] ab254361, ab314307 and Anti-FMRP antibody [EPR23852-90] ab259335 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum labelling Noradrenaline transporter with Anti-Noradrenaline transporter antibody [EPR23527-534] ab254361 at 1/100 (B), GPCR GPR17 with ab314307 at 1/2000 dilution (C) and FMRP with Anti-FMRP antibody [EPR23852-90] ab259335 at 1/10000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A: merged staining of anti-Noradrenaline transporter (magenta; Opal™690), anti-GPCR GPR17 (green; Opal™520) and anti-FMRP (gray; Opal™570) on rat cerebrum.

Panel B: anti-Noradrenaline transporter staining nerves in rat cerebrum.

Panel C: anti-GPCR GPR17 staining oligodendrocytes in rat cerebrum.

Panel D: anti-FMRP staining neurons in rat cerebrum.

The section was incubated in three rounds of staining: in the order of Anti-Noradrenaline transporter antibody [EPR23527-534] ab254361, ab314307 and Anti-FMRP antibody [EPR23852-90] ab259335 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum labelling Noradrenaline transporter with Anti-Noradrenaline transporter antibody [EPR23527-534] ab254361 at 1/100 (B), GPCR GPR17 with ab314307 at 1/2000 dilution (C) and FMRP with Anti-FMRP antibody [EPR23852-90] ab259335 at 1/10000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A: merged staining of anti-Noradrenaline transporter (magenta; Opal™690), anti-GPCR GPR17 (green; Opal™520) and anti-FMRP (gray; Opal™570) on mouse cerebrum.

Panel B: anti-Noradrenaline transporter staining nerves in mouse cerebrum.

Panel C: anti-GPCR GPR17 staining oligodendrocytes in mouse cerebrum.

Panel D: anti-FMRP staining neurons in mouse cerebrum.

The section was incubated in three rounds of staining: in the order of Anti-Noradrenaline transporter antibody [EPR23527-534] ab254361, ab314307 and Anti-FMRP antibody [EPR23852-90] ab259335 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling GPCR GPR17 with ab314307 at 1/2000 (0.259 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on rat liver. The section was incubated with ab314307 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling GPCR GPR17 with ab314307 at 1/2000 (0.259 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on mouse liver. The section was incubated with ab314307 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling GPCR GPR17 with ab314307 at 1/500 (1.034 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human skeletal muscle. The section was incubated with ab314307 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling GPCR GPR17 with ab314307 at 1/500 (1.034 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human liver. The section was incubated with ab314307 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling GPCR GPR17 with ab314307 at 1/2000 (0.259 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on oligodendrocytes of rat cerebrum. The section was incubated with ab314307 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling GPCR GPR17 with ab314307 at 1/2000 (0.259 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on oligodendrocytes of mouse cerebrum. The section was incubated with ab314307 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
Immunohistochemical analysis of paraffin-embedded human spinal cord tissue labeling GPCR GPR17 with ab314307 at 1/500 (1.034 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on oligodendrocytes of human spinal cord. The section was incubated with ab314307 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling GPCR GPR17 with ab314307 at 1/500 (1.034 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on oligodendrocytes of human cerebrum. The section was incubated with ab314307 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Anti-GPCR GPR17 antibody [EPR26422-118]

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Immunogen

Reactivity data

IHC-P

TestedTested

IP

TestedTested

ExpectedExpected

ICC/IF

TestedTested

ExpectedExpected

mIHC

TestedTested

WB

Not recommendedNot recommended

Flow Cyt

Not recommendedNot recommended

Associated Products

1 product for Alternative Version

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Product promise

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Predicted

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23 product images

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Immunoprecipitation - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Multiplex immunohistochemistry - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR17 antibody [EPR26422-118] (ab314307)

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Product protocols

Tested
Tested

Tested
Tested

Expected
Expected

Tested
Tested

Expected
Expected

Tested
Tested

Not recommended
Not recommended

Not recommended
Not recommended