Rabbit Polyclonal GPCR GPR18 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human GPR18.
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Publications
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- Frontiers in cell and developmental biology 11:11872532023Gpr18 agonist dampens inflammation, enhances myogenesis, and restores muscle function in models of Duchenne muscular dystrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunio Dort,Zakaria Orfi,Melissa Fiscaletti,Philippe M Campeau,Nicolas A DumontPubMed 37645248
- JCI insight 6:2021Inflammation resolution circuits are uncoupled in acute sepsis and correlate with clinical severity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBakr Jundi,Do-Hyun Lee,Hyungkook Jeon,Melody G Duvall,Julie Nijmeh,Raja-Elie E Abdulnour,Mayra Pinilla-Vera,Rebecca M Baron,Jongyoon Han,Joel Voldman,Bruce D LevyPubMed 34166226
- International journal of molecular sciences 20:2019Omega-3 Fatty Acid-Derived Resolvin D2 Regulates Human Placental Vascular Smooth Muscle and Extravillous Trophoblast Activities.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArzu Ulu et. al.PubMed 31500240
- The Journal of biological chemistry 289:13079-1002014The endocannabinoid/endovanilloid N-arachidonoyl dopamine (NADA) and synthetic cannabinoid WIN55,212-2 abate the inflammatory activation of human endothelial cells.Applications:WBReactive species:HumanKevin Wilhelmsen,Samira Khakpour,Alphonso Tran,Kayla Sheehan,Mark Schumacher,Fengyun Xu,Judith HellmanPubMed 24644287
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human GPR18. The exact immunogen used to generate this antibody is proprietary information. Database link Q14330
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Associated Products
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Target data
Function
Receptor for endocannabinoid N-arachidonyl glycine (NAGly) (PubMed:16844083, PubMed:24762058, PubMed:27572937). However, conflicting results about the role of NAGly as an agonist are reported (PubMed:27018161). Can also be activated by plant-derived and synthetic cannabinoid agonists (PubMed:24762058). The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase (PubMed:16844083). May contribute to regulation of the immune system. Is required for normal homeostasis of CD8+ subsets of intraepithelial lymphocytes (IELs) (CD8alphaalpha and CD8alphabeta IELs)in small intstine by supporting preferential migration of CD8alphaalpha T-cells to intraepithelial compartment over lamina propria compartment, and by mediating their reconstitution into small intestine after bone marrow transplant (By similarity). Plays a role in hypotensive responses, mediating reduction in intraocular and blood pressure (By similarity). Mediates NAGly-induced process of reorganization of actin filaments and induction of acrosomal exocytosis (PubMed:27572937).
Alternative names
GPCRW, GPR18, N-arachidonyl glycine receptor, NAGly receptor, G-protein coupled receptor 18
Recommended products
Rabbit Polyclonal GPCR GPR18 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human GPR18.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human GPR18. The exact immunogen used to generate this antibody is proprietary information. Database link Q14330
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GPR18 also known as N-arachidonyl glycine receptor is a G protein-coupled receptor (GPCR) found in various human tissues. It has an approximate mass of 40 kDa. This receptor is expressed most notably in immune cells including lymphocytes and macrophages as well as in the spleen and thymus. In addition to immune tissues GPR18 is also present in the heart brain and gastrointestinal system.
- Biological function summary
This receptor plays a role in cellular signaling as part of the cardiovascular and immune system regulation. GPR18 binds specific ligands such as N-arachidonoyl glycine facilitating intracellular communication and response to environmental changes. It does not function alone but integrates into GPCR complexes acting in concert with other receptors to regulate physiological functions like inflammation and cell migration.
- Pathways
The involvement of GPR18 in the endocannabinoid signaling and in the immune system makes it an essential player. It interfaces significantly with the endocannabinoid system influencing pathways like the calcium signaling pathway and possibly the MAPK signaling pathway. GPR18 interacts with proteins such as CB2 (cannabinoid receptor 2) to modulate immune responses inflammation and other critical cellular processes.
- Associated diseases and disorders
GPR18 is associated with conditions like inflammation and cardiovascular diseases. Changes in its expression or function can contribute to the development of these health issues. The receptor's interaction with proteins like CB2 becomes evident in these contexts where it may modulate disease progression and provide potential therapeutic targets for treating inflammation-related disorders or heart diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-GPCR GPR18 antibody (ab76258)
All lanes: Western blot - Anti-GPCR GPR18 antibody (ab76258) at 1/500 dilution
Lane 1: Extracts from HT-29 cells at 5 µg
Lane 2: Extracts from HT-29 cells plus 5µg immunizing peptide at 5 µg
Predicted band size: 38 kDa
Observed band size: 117 kDa, 38 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR18 antibody (ab76258)
Immunofluorescence analysis of HeLa cells using ab76258 at a 1/500 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
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