AB249825

Anti-GPCR GPR18 antibody [EPR12359] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal GPCR GPR18 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

GPCRW, GPR18, N-arachidonyl glycine receptor, NAGly receptor, G-protein coupled receptor 18

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Supplier Data

Western blot - Anti-GPCR GPR18 antibody [EPR12359] - BSA and Azide free (AB249825)

This data was developed using ab174835, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GPCR GPR18 antibody [EPR12359] (<a href='/en-us/products/primary-antibodies/gpcr-gpr18-antibody-epr12359-ab174835'>ab174835</a>) at 1/1000 dilution

Lane 1:

HuT-78 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 38 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12359

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab249825 is the carrier-free version of ab174835.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPR18 also known as N-arachidonyl glycine receptor is a G protein-coupled receptor (GPCR) found in various human tissues. It has an approximate mass of 40 kDa. This receptor is expressed most notably in immune cells including lymphocytes and macrophages as well as in the spleen and thymus. In addition to immune tissues GPR18 is also present in the heart brain and gastrointestinal system.

Biological function summary

This receptor plays a role in cellular signaling as part of the cardiovascular and immune system regulation. GPR18 binds specific ligands such as N-arachidonoyl glycine facilitating intracellular communication and response to environmental changes. It does not function alone but integrates into GPCR complexes acting in concert with other receptors to regulate physiological functions like inflammation and cell migration.

Pathways

The involvement of GPR18 in the endocannabinoid signaling and in the immune system makes it an essential player. It interfaces significantly with the endocannabinoid system influencing pathways like the calcium signaling pathway and possibly the MAPK signaling pathway. GPR18 interacts with proteins such as CB2 (cannabinoid receptor 2) to modulate immune responses inflammation and other critical cellular processes.

GPR18 is associated with conditions like inflammation and cardiovascular diseases. Changes in its expression or function can contribute to the development of these health issues. The receptor's interaction with proteins like CB2 becomes evident in these contexts where it may modulate disease progression and provide potential therapeutic targets for treating inflammation-related disorders or heart diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for endocannabinoid N-arachidonyl glycine (NAGly) (PubMed : 16844083, PubMed : 24762058, PubMed : 27572937). However, conflicting results about the role of NAGly as an agonist are reported (PubMed : 27018161). Can also be activated by plant-derived and synthetic cannabinoid agonists (PubMed : 24762058). The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase (PubMed : 16844083). May contribute to regulation of the immune system. Is required for normal homeostasis of CD8+ subsets of intraepithelial lymphocytes (IELs) (CD8alphaalpha and CD8alphabeta IELs)in small intstine by supporting preferential migration of CD8alphaalpha T-cells to intraepithelial compartment over lamina propria compartment, and by mediating their reconstitution into small intestine after bone marrow transplant (By similarity). Plays a role in hypotensive responses, mediating reduction in intraocular and blood pressure (By similarity). Mediates NAGly-induced process of reorganization of actin filaments and induction of acrosomal exocytosis (PubMed : 27572937).

See full target information GPR18

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GPCR GPR18 antibody [EPR12359] - BSA and Azide free

Western blot - Anti-GPCR GPR18 antibody [EPR12359] - BSA and Azide free (AB249825)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise