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AB75559

Anti-GPCR GPR20 antibody

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(1 Publication)

Rabbit Polyclonal GPCR GPR20 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human G-protein coupled receptor 20.

View Alternative Names

G-protein coupled receptor 20, GPR20

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR20 antibody (AB75559)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR20 antibody (AB75559)

Left panel : immunofluorescence analysis of A549 cells, using 1/500 ab75559; Right panel : same, after pre-incubation with the immunizing peptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR20 antibody (AB75559)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR20 antibody (AB75559)

Left panel : immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab75559; Right panel : same, after pre-incubation with the immunizing peptide.

Western blot - Anti-GPCR GPR20 antibody (AB75559)
  • WB

Unknown

Western blot - Anti-GPCR GPR20 antibody (AB75559)

All lanes:

Western blot - Anti-GPCR GPR20 antibody (ab75559) at 1/500 dilution

Lane 1:

extracts from LOVO cells at 20 µg

Lane 2:

extracts from LOVO cells at 20 µg with immunizing peptide

Predicted band size: 39 kDa

Observed band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human G-protein coupled receptor 20. The exact immunogen used to generate this antibody is proprietary information.

Q99678

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The G protein-coupled receptor GPR20 also known as GPR20 is a member of the GPCR family characterized by its seven-transmembrane domains. This receptor has a mass of approximately 37 kDa. GPR20 is an orphan receptor meaning its endogenous ligand is not well-defined. The receptor expresses most highly in the gastrointestinal tract and central nervous system although expression in other tissues can also occur. As an orphan GPCR GPR20 can influence intracellular signaling pathways through activation of G proteins.
Biological function summary

GPR20 plays a role in intracellular signaling pathways involving cyclic AMP (cAMP) and phosphatidylinositol. Although not part of a complex GPR20 can interact with various G proteins to transmit signals from the outside to the inside of the cell. This influences various cellular responses such as cell proliferation differentiation and survival. The exact biological functions of GPR20 are still under investigation but can influence physiological processes in the tissues where it is expressed.

Pathways

GPR20 integrates into signaling pathways related to cAMP production and phosphatidylinositol metabolism. GPR20 influences the cAMP signaling pathway potentially impacting processes like neurotransmission. Through this pathway GPR20 can interact with other proteins like adenylate cyclases and various G protein subunits sharing pathways with other GPCRs but exhibiting specific roles based on its tissue distribution and receptor specificity.

Research indicates that GPR20 may have a connection to cancer especially gastrointestinal cancer. The high expression of GPR20 in the gastrointestinal tract makes it a potential target for understanding cancer cell proliferation in this region. Additionally GPR20 might relate to neurological disorders because of its presence in the central nervous system. Its signaling pathways intersect with other proteins like beta-arrestins which play roles in regulating receptor desensitization potentially influencing cancer progression or neurological disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Orphan receptor with constitutive G(i) signaling activity that activate cyclic AMP.
See full target information G-protein coupled receptor 20
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Annals of dermatology 31:154-163 PubMed33911564

2019

Neuropeptides Profile and Increased Innervation in Becker's Nevus.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Seok Kim,Myeong Jin Park,Hye Young Kang,Seung Phil Hong,Byung Cheol Park,Myung Hwa Kim
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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