Rabbit Polyclonal GPCR GPR3 antibody. Extracellular domain. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human GPR3.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.099% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human GPR3. The exact immunogen used to generate this antibody is proprietary information. Database link P46089
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Gorilla | Predicted |
| Hamster | Predicted |
| Monkey | Predicted |
| Rabbit | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 6 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Hamster, Monkey, Gorilla | Dilution info - | Notes - |
Target data
Function
Constitutively active G-protein coupled receptor that maintains high 3'-5'-cyclic adenosine monophosphate (cAMP) levels that a plays a role in serveral processes including meiotic arrest in oocytes or neuronal development via activation of numerous intracellular signaling pathways. Acts as an essential activator of thermogenic adipocytes and drives thermogenesis via its intrinsic G(s)-coupling activity without the requirement of a ligand (PubMed:34048700). Has a potential role in modulating a number of brain functions, including behavioral responses to stress (By similarity), amyloid-beta peptide generation in neurons (By similarity). Stimulates neurite outgrowth in cerebellar granular neurons modulated via PKA, ERK, and most strongly PI3K-mediated signaling pathways (By similarity).
Alternative names
ACCA, GPR3, G-protein coupled receptor 3, ACCA orphan receptor
Recommended products
Rabbit Polyclonal GPCR GPR3 antibody. Extracellular domain. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human GPR3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.099% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human GPR3. The exact immunogen used to generate this antibody is proprietary information. Database link P46089
- Purification technique
- Affinity purification Immunogen
- Specificity
BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GPR3 also known as G protein-coupled receptor 3 is a member of the GPCR family. This receptor weighs approximately 37 kDa and exists in the cell plasma membrane where it triggers intracellular signaling pathways by interacting with G proteins. Expressed widely in the central nervous system GPR3 plays a mechanical role by activating adenylate cyclase and increasing cyclic AMP (cAMP) levels without any ligand binding a unique characteristic of this receptor.
- Biological function summary
The function of GPR3 influences neural processes through its regulation of cAMP signaling. GPR3 is notably a part of signaling cascades within neuronal tissues and impacts processes such as neurogenesis and synaptic transmission. The receptor does not act alone; cooperation with other proteins in cAMP-independent pathways suggests possible involvement in broader complexes affecting brain function.
- Pathways
GPR3 contributes significantly to the modulation of signaling via pathways like the cAMP/PKA pathway and may overlap or interact with the Wnt signaling pathway. These pathways include proteins like adenylate cyclase which GPR3 activates playing roles in cellular communication and signal transduction. Through these systems GPR3 impacts various physiological processes especially in the central nervous system intertwining with additional GPCR family members and intracellular signaling proteins.
- Associated diseases and disorders
GPR3 reveals links to Alzheimer's disease and depression potentially due to its regulation of cAMP and related impacts on brain function. The receptor's interaction with proteins in these conditions suggests a possible contribution to disease pathology. Specifically its relationship with beta-amyloid proteins in Alzheimer's elucidates a connection of GPR3 to neurodegenerative pathways. Similarly altered GPR3 function and its effects on serotonin pathways provide insights into depression mechanisms and therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR3 antibody - Extracellular domain (ab219786)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain, neurons and glia tissue labeling GPCR GPR3 with ab219786 at 6 μg/mL.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR3 antibody - Extracellular domain (ab219786)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovary carcinoma tissue labeling GPCR GPR3 with ab219786 at 6 μg/mL.
Downloads
Product protocols
- Visit the general protocols
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