JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB219786

Anti-GPCR GPR3 antibody - Extracellular domain

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal GPCR GPR3 antibody. Extracellular domain. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GPR3.

View Alternative Names

ACCA, GPR3, G-protein coupled receptor 3, ACCA orphan receptor

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR3 antibody - Extracellular domain (AB219786)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR3 antibody - Extracellular domain (AB219786)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovary carcinoma tissue labeling GPCR GPR3 with ab219786 at 6 μg/mL.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR3 antibody - Extracellular domain (AB219786)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR3 antibody - Extracellular domain (AB219786)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain, neurons and glia tissue labeling GPCR GPR3 with ab219786 at 6 μg/mL.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human GPR3. The exact immunogen used to generate this antibody is proprietary information.

P46089

Specificity

BLAST analysis of the peptide immunogen showed no homology with other human proteins.

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB83353

Recombinant HIV1 tat protein

  • 0
  • 0
Proteins & Peptides

AB119880

Recombinant HPV16 L1 protein

Electron Microscopy - Recombinant HPV16 L1 protein (AB119880)

  • 0
  • 0
Proteins & Peptides

AB319985

Recombinant Mouse Prealbumin Protein Standard (His tag)

Sandwich ELISA - Recombinant Mouse Prealbumin Protein Standard (His tag) (AB319985)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "6 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.099% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPR3 also known as G protein-coupled receptor 3 is a member of the GPCR family. This receptor weighs approximately 37 kDa and exists in the cell plasma membrane where it triggers intracellular signaling pathways by interacting with G proteins. Expressed widely in the central nervous system GPR3 plays a mechanical role by activating adenylate cyclase and increasing cyclic AMP (cAMP) levels without any ligand binding a unique characteristic of this receptor.
Biological function summary

The function of GPR3 influences neural processes through its regulation of cAMP signaling. GPR3 is notably a part of signaling cascades within neuronal tissues and impacts processes such as neurogenesis and synaptic transmission. The receptor does not act alone; cooperation with other proteins in cAMP-independent pathways suggests possible involvement in broader complexes affecting brain function.

Pathways

GPR3 contributes significantly to the modulation of signaling via pathways like the cAMP/PKA pathway and may overlap or interact with the Wnt signaling pathway. These pathways include proteins like adenylate cyclase which GPR3 activates playing roles in cellular communication and signal transduction. Through these systems GPR3 impacts various physiological processes especially in the central nervous system intertwining with additional GPCR family members and intracellular signaling proteins.

GPR3 reveals links to Alzheimer's disease and depression potentially due to its regulation of cAMP and related impacts on brain function. The receptor's interaction with proteins in these conditions suggests a possible contribution to disease pathology. Specifically its relationship with beta-amyloid proteins in Alzheimer's elucidates a connection of GPR3 to neurodegenerative pathways. Similarly altered GPR3 function and its effects on serotonin pathways provide insights into depression mechanisms and therapies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Constitutively active G-protein coupled receptor that maintains high cAMP levels and contributes to several processes, including meiotic arrest in oocytes and neuronal development, through activation of intracellular signaling pathways. Essential activator of thermogenic adipocytes, where it drives thermogenesis via constitutive G(s)-coupling activity in the absence of ligand (PubMed : 34048700). May be activated by lipid-derived agonists such as oleoylethanolamide (OEA), oleic acid or sphingosine 1-phosphate leading to activation of the G(s)/cAMP/PKA signaling pathway (PubMed : 12220620, PubMed : 38376112, PubMed : 38287117). Plays a potential role in modulating brain functions, including behavioral responses to stress and amyloid-beta peptide generation in neurons (By similarity). Stimulates neurite outgrowth in cerebellar granule neurons via PKA, ERK, and PI3K-mediated signaling pathways (By similarity).
See full target information GPR3
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 6: PubMed34032636

2021

T-bet+CD27+CD21- B cells poised for plasma cell differentiation during antibody-mediated rejection of kidney transplants.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Louis,Elodie Bailly,Camila Macedo,Louis Lau,Bala Ramaswami,Alexander Chang,Uma Chandran,Douglas Landsittel,Xinyan Gu,Geetha Chalasani,Adriana Zeevi,Parmjeet Randhawa,Harinder Singh,Carmen Lefaucheur,Diana Metes
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com