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AB188931

Anti-GPCR GPR4 antibody - C-terminal

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(2 Publications)

Rabbit Polyclonal GPCR GPR4 antibody. C-terminal. Suitable for IHC-P, ICC and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GPR4.

View Alternative Names

G-protein coupled receptor 4, hGPR4, G-protein coupled receptor 6C.l, GPR6C.l, GPR4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR4 antibody - C-terminal (AB188931)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR4 antibody - C-terminal (AB188931)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung (respiratory epithelium) tissue labeling GPCR GPR4 with ab188931 at 24 μg/ml.

Immunocytochemistry - Anti-GPCR GPR4 antibody - C-terminal (AB188931)
  • ICC

Supplier Data

Immunocytochemistry - Anti-GPCR GPR4 antibody - C-terminal (AB188931)

Immunocytochemical analysis of untransfected HEK293 cells.

Immunocytochemistry - Anti-GPCR GPR4 antibody - C-terminal (AB188931)
  • ICC

Supplier Data

Immunocytochemistry - Anti-GPCR GPR4 antibody - C-terminal (AB188931)

Immunocytochemical analysis of GPCR GPR4 transfected HEK293 cells labeling GPCR GPR4 with ab188931.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC, IHC-P

applications

Immunogen

Synthetic Peptide within Human GPR4. The exact immunogen used to generate this antibody is proprietary information.

P46093

Specificity

BLAST analysis of the peptide immunogen showed no homology with other Human proteins.

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Alternative Products

Primary Antibodies

AB188913

Anti-GPCR GPR4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR4 antibody (AB188913)

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Primary Antibodies

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Primary Antibodies

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Anti-GPCR GPR35 antibody

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Primary Antibodies

AB140795

Anti-GPR91 antibody - Cytoplasmic domain

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR91 antibody - Cytoplasmic domain (AB140795)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "24 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Bat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPR4 also known as G protein-coupled receptor 4 is a member of the GPCR family and plays a critical role in physiological processes. It has an approximate molecular mass of 41 kDa and is expressed in various tissues including the lung heart kidney and pancreatic tissues. GPR4 functions as a proton-sensing receptor which allows it to detect and respond to changes in pH levels providing essential feedback for cellular homeostasis.
Biological function summary

The proton-sensing ability of GPR4 influences numerous cellular activities including angiogenesis inflammatory responses and cell proliferation. GPR4 does not form part of any well-known protein complex but it partners with several intracellular signaling molecules like G proteins which transduce the extracellular pH changes into intracellular signals. The receptor's activation leads to downstream effects that affect cell physiology and contribute to adaptation to microenvironmental changes.

Pathways

The function of GPR4 integrates into the acid-sensing signaling and inflammatory response pathways. In the acid-sensing signaling pathway GPR4 works closely with other proton-sensitive receptors to regulate vascular and renal functions adjusting cellular activities according to pH variations. Within the inflammatory response pathway GPR4 interacts with proteins involved in immune cell recruitment and activation linking changes in pH to immune modulation and inflammatory responses.

The activity of GPR4 has a significant connection to cancer and inflammatory diseases. The overexpression of GPR4 in certain tumors associates it with cancer progression where it influences tumor microenvironment acidification and consequent cell invasion and metastasis. In inflammatory diseases GPR4 modulates immune responses correlating with conditions like rheumatoid arthritis where it connects to other immune receptors and inflammatory mediators.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Proton-sensing G-protein coupled receptor activated by extracellular pH, which is required to monitor pH changes and generate adaptive reactions (PubMed : 12955148, PubMed : 17462861, PubMed : 33478938, PubMed : 39753132, PubMed : 39799123, PubMed : 40211064, PubMed : 40215959, PubMed : 40215960). Activated by an optimal pH of 6.8-7.2 (PubMed : 12955148, PubMed : 17462861, PubMed : 39753132). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed : 39753132, PubMed : 40211064, PubMed : 40215960). GPR4 is mainly coupled to G(s) G proteins and mediates activation of adenylate cyclase activity (PubMed : 12955148, PubMed : 17462861, PubMed : 20211729, PubMed : 22110680, PubMed : 39753132, PubMed : 40211064, PubMed : 40215960). May also couple with G(i), G(q) and G(12)/G(13) G proteins (PubMed : 12955148, PubMed : 17462861, PubMed : 20211729, PubMed : 22110680). Acts as a key regulator of respiratory sensitivity to CO2/H(+) in brain retrotrapezoid nucleus neurons : acts by mediating detection of protons generated by the formation of carbonic acid in the blood, an important mechanism to impulse to breathe (By similarity). Also acts as a regulator of acid secretion in the kidney collecting duct by maintaining acid-base homeostasis in the kidney (By similarity). Acidosis-induced GPR4 activation increases paracellular gap formation and permeability of vascular endothelial cells, possibly through the G(12)/G(13)/Rho GTPase signaling pathway (PubMed : 32058960).
See full target information GPR4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Naunyn-Schmiedeberg's archives of pharmacology 397:7187-7200 PubMed38652276

2024

The thrombin receptor PAR4 supports visceral adipose tissue inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Sonja Kleeschulte,Vivien Fischinger,Lisa Öhlke,Johannes Bode,Markus Kamler,Dobromir Dobrev,Maria Grandoch,Anke C Fender

EBioMedicine 48:264-276 PubMed31530502

2019

Increased proton-sensing receptor GPR4 signalling promotes colorectal cancer progression by activating the hippo pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Minhao Yu,Ran Cui,Yizhou Huang,Yang Luo,Shaolan Qin,Ming Zhong
View all publications
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