Rabbit Recombinant Monoclonal GPCR GPR4 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR4 antibody [EPR27342-7] - BSA and Azide free (AB307364), expandable thumbnail](https://content.abcam.com/products/images/gpcr-gpr4-antibody-epr27342-7-bsa-and-azide-free-ab307364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405553.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR4 antibody [EPR27342-7] - BSA and Azide free (AB307364)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR4 antibody [EPR27342-7] - BSA and Azide free (AB307364), expandable thumbnail](https://content.abcam.com/products/images/gpcr-gpr4-antibody-epr27342-7-bsa-and-azide-free-ab307364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405552.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR4 antibody [EPR27342-7] - BSA and Azide free (AB307364)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt (Intra) | IHC-P | WB | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Target data
Function
Proton-sensing G-protein coupled receptor couples to multiple intracellular signaling pathways, including GNAS/cAMP, GNAQ/phospholipase C (PLC), and GNA12/GNA13/Rho pathways (PubMed:12955148, PubMed:17462861, PubMed:20211729, PubMed:22110680). Acidosis-induced GPR4 activation increases paracellular gap formation and permeability of vascular endothelial cells through the GNA12/GNA13/Rho GTPase signaling pathway (PubMed:32058960). In the brain may mediate central respiratory sensitivity to CO(2)H(+) (By similarity).
Alternative names
G-protein coupled receptor 4, G-protein coupled receptor 6C.l, GPR6C.l, GPR4
Recommended products
Rabbit Recombinant Monoclonal GPCR GPR4 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27342-7
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GPR4 also known as G protein-coupled receptor 4 is a member of the GPCR family and plays a critical role in physiological processes. It has an approximate molecular mass of 41 kDa and is expressed in various tissues including the lung heart kidney and pancreatic tissues. GPR4 functions as a proton-sensing receptor which allows it to detect and respond to changes in pH levels providing essential feedback for cellular homeostasis.
- Biological function summary
The proton-sensing ability of GPR4 influences numerous cellular activities including angiogenesis inflammatory responses and cell proliferation. GPR4 does not form part of any well-known protein complex but it partners with several intracellular signaling molecules like G proteins which transduce the extracellular pH changes into intracellular signals. The receptor's activation leads to downstream effects that affect cell physiology and contribute to adaptation to microenvironmental changes.
- Pathways
The function of GPR4 integrates into the acid-sensing signaling and inflammatory response pathways. In the acid-sensing signaling pathway GPR4 works closely with other proton-sensitive receptors to regulate vascular and renal functions adjusting cellular activities according to pH variations. Within the inflammatory response pathway GPR4 interacts with proteins involved in immune cell recruitment and activation linking changes in pH to immune modulation and inflammatory responses.
- Associated diseases and disorders
The activity of GPR4 has a significant connection to cancer and inflammatory diseases. The overexpression of GPR4 in certain tumors associates it with cancer progression where it influences tumor microenvironment acidification and consequent cell invasion and metastasis. In inflammatory diseases GPR4 modulates immune responses correlating with conditions like rheumatoid arthritis where it connects to other immune receptors and inflammatory mediators.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR4 antibody [EPR27342-7] - BSA and Azide free (ab307364), expandable thumbnail](https://content.abcam.com/products/images/gpcr-gpr4-antibody-epr27342-7-bsa-and-azide-free-ab307364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405553.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR4 antibody [EPR27342-7] - BSA and Azide free (ab307364)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR4 antibody [EPR27342-7] - BSA and Azide free (ab307364)
This data was developed using Anti-GPCR GPR4 antibody [EPR27342-7] ab307363, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling GPCR GPR4 with Anti-GPCR GPR4 antibody [EPR27342-7] ab307363 at 1/1000 (0.491 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on endothelial cells of human placenta. The section was incubated with Anti-GPCR GPR4 antibody [EPR27342-7] ab307363 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR4 antibody [EPR27342-7] - BSA and Azide free (ab307364), expandable thumbnail](https://content.abcam.com/products/images/gpcr-gpr4-antibody-epr27342-7-bsa-and-azide-free-ab307364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405552.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR4 antibody [EPR27342-7] - BSA and Azide free (ab307364)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR4 antibody [EPR27342-7] - BSA and Azide free (ab307364)
This data was developed using Anti-GPCR GPR4 antibody [EPR27342-7] ab307363, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human breast carcino tissue labeling GPCR GPR4 with Anti-GPCR GPR4 antibody [EPR27342-7] ab307363 at 1/1000 (0.491 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on endothelial cells of human breast carcinoma. The section was incubated with Anti-GPCR GPR4 antibody [EPR27342-7] ab307363 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
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