Rabbit Polyclonal GPCR GPR40 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FFAR1 aa 100-200.
View Alternative Names
GPR40, FFAR1, Free fatty acid receptor 1, G-protein coupled receptor 40
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR40 antibody (AB236285)
Paraffin-embedded human brain tissue stained for GPCR GPR40 using ab236285 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR40 antibody (AB236285)
Paraffin-embedded human pancreas tissue stained for GPCR GPR40 using ab236285 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR40 antibody (AB236285)
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for GPCR GPR40 (green) using ab236285 at 1/133 dilution in ICC/IF. Counterstained with DAPI. The secondary antibody is Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).
Reactivity data
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Supplementary information
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Biological function summary
GPR40 is integral to the regulation of glucose homeostasis. This receptor functions as a part of a complex that facilitates the enhancement of insulin secretion in response to elevated blood glucose levels. When activated GPR40 modulates the release of insulin from pancreatic beta cells contributing significantly to glucose metabolism. This function places GPR40 as an important player in maintaining metabolic balance especially in response to dietary fatty acids.
Pathways
GPR40's role in glucose homeostasis places it within the insulin signaling and fatty acid metabolism pathways. It interacts with proteins like Gq and phospholipase C which transmit signals leading to insulin secretion. These pathways highlight the functional role GPR40 plays in linking nutrient sensing to hormone secretion which positions it alongside other receptors that mediate metabolic processes such as glucose-dependent insulinotropic polypeptide (GIP).
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Publications (4)
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Cancer cell international 23:126 PubMed37355607
2023
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Unspecified reactive species
Frontiers in cellular neuroscience 17:1081190 PubMed37252191
2023
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Species
Unspecified reactive species
Frontiers in immunology 14:1114321 PubMed36911720
2023
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Unspecified reactive species
Drug design, development and therapy 14:5143-5153 PubMed33262575
2020
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Species
Unspecified reactive species
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