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Mouse Monoclonal GPCR GPR48/LGR4 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human LGR4.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR48/LGR4 antibody [6G8.B3.G5.C3] (AB106089), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR48/LGR4 antibody [6G8.B3.G5.C3] (AB106089), expandable thumbnail

Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.42% Tripotassium orthophosphate

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Human LGR4. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BXB1

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested
Chimpanzee
Predicted
Macaque monkey
Predicted
Orangutan
Predicted

Tested
Tested

Species

Human

Dilution info

5 µg/mL

Notes

Do not perform antigen retrieval.

Predicted
Predicted

Species

Chimpanzee, Macaque monkey, Orangutan

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Its function as activator of the Wnt signaling pathway is required for the development of various organs, including liver, kidney, intestine, bone, reproductive tract and eye. May also act as a receptor for norrin (NDP), such results however require additional confirmation in vivo. Required during spermatogenesis to activate the Wnt signaling pathway in peritubular myoid cells. Required for the maintenance of intestinal stem cells and Paneth cell differentiation in postnatal intestinal crypts. Acts as a regulator of bone formation and remodeling. Involved in kidney development; required for maintaining the ureteric bud in an undifferentiated state. Involved in the development of the anterior segment of the eye. Required during erythropoiesis. Also acts as a negative regulator of innate immunity by inhibiting TLR2/TLR4 associated pattern-recognition and pro-inflammatory cytokine production. Plays an important role in regulating the circadian rhythms of plasma lipids, partially through regulating the rhythmic expression of MTTP (By similarity). Required for proper development of GnRH neurons (gonadotropin-releasing hormone expressing neurons) that control the release of reproductive hormones from the pituitary gland (By similarity).

Alternative names

Recommended products

Mouse Monoclonal GPCR GPR48/LGR4 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human LGR4.

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Human LGR4. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BXB1
Clone number

6G8.B3.G5.C3

Purification technique

Affinity purification Protein A

Specificity

A BLAST analysis was used to suggest cross-reactivity with LGR4 from chimpanzee, orangutan and macaque based on 100% homology with the immunizing sequence. Cross-reactivity with LGR4 from other sources has not been determined.

Concentration
Loading...
Purification notes

ab106089 was purified from concentrated tissue culture supernate by Protein A chromatography.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Expiration date is one year from date of opening. Dilute only prior to immediate use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GPR48 also known as LGR4 is a member of the G protein-coupled receptor (GPCR) family. This receptor has a known molecular mass of approximately 105 kDa. It is expressed in various tissues including the liver kidneys intestines and reproductive organs. LGR4 is part of the large family of leucine-rich repeat-containing GPCRs and functions through the activation of distinct downstream signaling pathways which eventually lead to cellular responses important for physiological processes.

Biological function summary

The receptor engages in a wide range of functions through its interactions with specific ligands influencing cell proliferation differentiation and survival. LGR4 does not act alone but forms complexes with other receptors or signaling molecules to perform specific activities. It significantly impacts the development of the reproductive system and plays a role in bone formation and remodeling. The receptor contributes to cellular signaling that drives essential biological processes by modulating the activity of various downstream components.

Pathways

The LGR4 receptor is actively involved in the Wnt signaling pathway and RANKL pathway both essential pathways for development and tissue homeostasis. Within the Wnt signaling pathway LGR4 interacts with proteins like LRP6 and Frizzled receptors to regulate gene expression and cellular proliferation. In the RANKL pathway it orchestrates osteoclast differentiation which is important for bone metabolism. The receptor's ability to modulate these pathways highlights its intricate role in maintaining cellular and structural integrity in the body.

Associated diseases and disorders

Aberrations in LGR4 signaling have associations with various conditions such as osteoporosis and cancer. In osteoporosis disrupted signaling affects bone density and strength leading to increased fracture risk. In cancers altered LGR4 function or expression can contribute to tumorigenesis impacting cancer progression and metastasis. The receptor can relate to proteins such as RANK and RANKL in bone diseases and interacts with other signaling molecules to affect cancer pathways suggesting its potential as a therapeutic target in these disorders.

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2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR48/LGR4 antibody [6G8.B3.G5.C3] (ab106089), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR48/LGR4 antibody [6G8.B3.G5.C3] (ab106089)

    ab106089 at 5μg/ml staining GPCR GPR48/LGR4 in Formalin-fixed,Paraffin-embedded Human Brain cerebellum by Immunohistochemistry.
    The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR48/LGR4 antibody [6G8.B3.G5.C3] (ab106089), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR48/LGR4 antibody [6G8.B3.G5.C3] (ab106089)

    ab106089 at 5μg/ml staining GPCR GPR48/LGR4 in Formalin-fixed,Paraffin-embedded Human Pancreas, ilset tissue by Immunoohistochemistry. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

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Product protocols

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