Rabbit Polyclonal GPCR GPR48/LGR4 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.
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Publications
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- Journal of oncology 2022:17740952022Metformin Suppresses Hepatocellular Carcinoma through Regulating Alternative Splicing of LGR4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHan Zhuo,Shuying Miao,Zhenquan Jin,Deming Zhu,Zhenggang Xu,Dongwei Sun,Jie Ji,Zhongming TanPubMed 36385965
- iScience 25:1051842022RSPO2 promotes progression of ovarian cancer through dual receptor-mediated FAK/Src signaling activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRulu Pan,Yan Yu,Haiyan Zhu,Wenyi Zhang,Yuan Qin,Lin Ye,Juji Dai,Ren Huang,Xinyan Peng,Siqi Ye,Ziqi Lin,Shishun Huang,Shuyi Chong,Liting Lu,Xincheng LuPubMed 36217544
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
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Target data
Function
Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Its function as activator of the Wnt signaling pathway is required for the development of various organs, including liver, kidney, intestine, bone, reproductive tract and eye. May also act as a receptor for norrin (NDP), such results however require additional confirmation in vivo. Required during spermatogenesis to activate the Wnt signaling pathway in peritubular myoid cells. Required for the maintenance of intestinal stem cells and Paneth cell differentiation in postnatal intestinal crypts. Acts as a regulator of bone formation and remodeling. Involved in kidney development; required for maintaining the ureteric bud in an undifferentiated state. Involved in the development of the anterior segment of the eye. Required during erythropoiesis. Also acts as a negative regulator of innate immunity by inhibiting TLR2/TLR4 associated pattern-recognition and pro-inflammatory cytokine production. Plays an important role in regulating the circadian rhythms of plasma lipids, partially through regulating the rhythmic expression of MTTP (By similarity). Required for proper development of GnRH neurons (gonadotropin-releasing hormone expressing neurons) that control the release of reproductive hormones from the pituitary gland (By similarity).
Alternative names
GPR48, LGR4, Leucine-rich repeat-containing G-protein coupled receptor 4, G-protein coupled receptor 48
Recommended products
Rabbit Polyclonal GPCR GPR48/LGR4 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GPR48 also known as LGR4 is a member of the G protein-coupled receptor (GPCR) family. This receptor has a known molecular mass of approximately 105 kDa. It is expressed in various tissues including the liver kidneys intestines and reproductive organs. LGR4 is part of the large family of leucine-rich repeat-containing GPCRs and functions through the activation of distinct downstream signaling pathways which eventually lead to cellular responses important for physiological processes.
- Biological function summary
The receptor engages in a wide range of functions through its interactions with specific ligands influencing cell proliferation differentiation and survival. LGR4 does not act alone but forms complexes with other receptors or signaling molecules to perform specific activities. It significantly impacts the development of the reproductive system and plays a role in bone formation and remodeling. The receptor contributes to cellular signaling that drives essential biological processes by modulating the activity of various downstream components.
- Pathways
The LGR4 receptor is actively involved in the Wnt signaling pathway and RANKL pathway both essential pathways for development and tissue homeostasis. Within the Wnt signaling pathway LGR4 interacts with proteins like LRP6 and Frizzled receptors to regulate gene expression and cellular proliferation. In the RANKL pathway it orchestrates osteoclast differentiation which is important for bone metabolism. The receptor's ability to modulate these pathways highlights its intricate role in maintaining cellular and structural integrity in the body.
- Associated diseases and disorders
Aberrations in LGR4 signaling have associations with various conditions such as osteoporosis and cancer. In osteoporosis disrupted signaling affects bone density and strength leading to increased fracture risk. In cancers altered LGR4 function or expression can contribute to tumorigenesis impacting cancer progression and metastasis. The receptor can relate to proteins such as RANK and RANKL in bone diseases and interacts with other signaling molecules to affect cancer pathways suggesting its potential as a therapeutic target in these disorders.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-GPCR GPR48/LGR4 antibody (ab75501)
All lanes: Western blot - Anti-GPCR GPR48/LGR4 antibody (ab75501) at 1/500 dilution
Lane 1: Jurkat cell extract at 30 µg
Lane 2: Jurkat cell extract at 30 µg with immunising peptide
Predicted band size: 104 kDa
Observed band size: 104 kDa
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