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Rabbit Recombinant Monoclonal GPCR GPR48/LGR4 antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human, Mouse samples.

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Images

Western blot - Anti-GPCR GPR48/LGR4 antibody [EPR26351-175] (AB321789), expandable thumbnail
  • Western blot - Anti-GPCR GPR48/LGR4 antibody [EPR26351-175] (AB321789), expandable thumbnail
  • Western blot - Anti-GPCR GPR48/LGR4 antibody [EPR26351-175] (AB321789), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Not recommended
Mouse
Tested
Not recommended
Rat
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Target data

Function

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Its function as activator of the Wnt signaling pathway is required for the development of various organs, including liver, kidney, intestine, bone, reproductive tract and eye. May also act as a receptor for norrin (NDP), such results however require additional confirmation in vivo. Required during spermatogenesis to activate the Wnt signaling pathway in peritubular myoid cells. Required for the maintenance of intestinal stem cells and Paneth cell differentiation in postnatal intestinal crypts. Acts as a regulator of bone formation and remodeling. Involved in kidney development; required for maintaining the ureteric bud in an undifferentiated state. Involved in the development of the anterior segment of the eye. Required during erythropoiesis. Also acts as a negative regulator of innate immunity by inhibiting TLR2/TLR4 associated pattern-recognition and pro-inflammatory cytokine production. Plays an important role in regulating the circadian rhythms of plasma lipids, partially through regulating the rhythmic expression of MTTP (By similarity). Required for proper development of GnRH neurons (gonadotropin-releasing hormone expressing neurons) that control the release of reproductive hormones from the pituitary gland (By similarity).

Alternative names

GPR48, LGR4, Leucine-rich repeat-containing G-protein coupled receptor 4, G-protein coupled receptor 48

Recommended products

Rabbit Recombinant Monoclonal GPCR GPR48/LGR4 antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26351-175
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GPR48 also known as LGR4 is a member of the G protein-coupled receptor (GPCR) family. This receptor has a known molecular mass of approximately 105 kDa. It is expressed in various tissues including the liver kidneys intestines and reproductive organs. LGR4 is part of the large family of leucine-rich repeat-containing GPCRs and functions through the activation of distinct downstream signaling pathways which eventually lead to cellular responses important for physiological processes.

Biological function summary

The receptor engages in a wide range of functions through its interactions with specific ligands influencing cell proliferation differentiation and survival. LGR4 does not act alone but forms complexes with other receptors or signaling molecules to perform specific activities. It significantly impacts the development of the reproductive system and plays a role in bone formation and remodeling. The receptor contributes to cellular signaling that drives essential biological processes by modulating the activity of various downstream components.

Pathways

The LGR4 receptor is actively involved in the Wnt signaling pathway and RANKL pathway both essential pathways for development and tissue homeostasis. Within the Wnt signaling pathway LGR4 interacts with proteins like LRP6 and Frizzled receptors to regulate gene expression and cellular proliferation. In the RANKL pathway it orchestrates osteoclast differentiation which is important for bone metabolism. The receptor's ability to modulate these pathways highlights its intricate role in maintaining cellular and structural integrity in the body.

Associated diseases and disorders

Aberrations in LGR4 signaling have associations with various conditions such as osteoporosis and cancer. In osteoporosis disrupted signaling affects bone density and strength leading to increased fracture risk. In cancers altered LGR4 function or expression can contribute to tumorigenesis impacting cancer progression and metastasis. The receptor can relate to proteins such as RANK and RANKL in bone diseases and interacts with other signaling molecules to affect cancer pathways suggesting its potential as a therapeutic target in these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-GPCR GPR48/LGR4 antibody [EPR26351-175] (ab321789), expandable thumbnail

    Western blot - Anti-GPCR GPR48/LGR4 antibody [EPR26351-175] (ab321789)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human GPCR LGR5.

    Samples are non-boiled as boiling may cause protein aggregation.

    All lanes: Western blot - Anti-GPCR GPR48/LGR4 antibody [EPR26351-175] (ab321789) at 1/1000 dilution

    Lane 1: His-tagged human GPCR GPR48/LGR4 fragment at 10 ng

    Lane 2: Human GPCR LGR5 fragment at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 60 kDa, 300 kDa

    Exposure time: 15s

  • Western blot - Anti-GPCR GPR48/LGR4 antibody [EPR26351-175] (ab321789), expandable thumbnail

    Western blot - Anti-GPCR GPR48/LGR4 antibody [EPR26351-175] (ab321789)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    GPCR GPR48/LGR4 is a glycoprotein of approximately 110-120 kDa and detected as a 100-kDa band after treated with Peptide:N-glycosidase F (PNGase F).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24205130, PMID: 11401528).

    Samples are non-boiled as boiling may cause protein aggregation.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-GPCR GPR48/LGR4 antibody [EPR26351-175] (ab321789) at 1/1000 dilution

    Lane 1: NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 2: Untreated A549 (human lung carcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 3: A549 treated with Peptide:N-glycosidase F (PNGase F) whole cell lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 100 kDa, 110-120 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-GPCR GPR48/LGR4 antibody [EPR26351-175] (ab321789), expandable thumbnail

    Western blot - Anti-GPCR GPR48/LGR4 antibody [EPR26351-175] (ab321789)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody is specific to GPCR GPR48/LGR4 and does not cross react with endogenous GPCR LGR6.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24205130, PMID: 11401528).

    Samples are non-boiled as boiling may cause protein aggregation.

    In Western blot, Anti-GPCR LGR6 antibody [EPR6874] (Anti-GPCR LGR6 antibody [EPR6874] ab126747) - Total protein control staining at 1/1000 dilution.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    The high-sensitivity ECL substrate allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-GPCR GPR48/LGR4 antibody [EPR26351-175] (ab321789) at 1/1000 dilution

    Lane 1: A549 (human lung carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 40 µg

    Lane 2: A549 transfected with siRNA specifically targeting GPCR GPR48/LGR4 whole cell lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 110-120 kDa, 36 kDa, 104 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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