AB203663

Anti-GPCR GPR55 antibody

Name: Anti-GPCR GPR55 antibody (ab203663) | Abcam
Brand: Abcam Limited
SKU: AB203663
Price: 430 USD
Availability: InStock

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(9 Publications)

Rabbit Polyclonal GPCR GPR55 antibody. Suitable for Flow Cyt, WB and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human GPR55 aa 150-200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

G-protein coupled receptor 55, GPR55

2 Images

Supplier Data

Western blot - Anti-GPCR GPR55 antibody (AB203663)

All lanes:

Western blot - Anti-GPCR GPR55 antibody (ab203663) at 1/200 dilution

Lane 1:

Mouse brain lysate

Lane 2:

Mouse embryo lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution

Predicted band size: 37 kDa

Observed band size: 37 kDa

false

Unknown

Western blot - Anti-GPCR GPR55 antibody (AB203663)

All lanes:

Western blot - Anti-GPCR GPR55 antibody (ab203663)

All lanes:

U87MG cell lysates

Secondary

All lanes:

A conjugated secondary antibody at 1/20000 dilution

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

Flow Cyt, WB

applications

Immunogen

Synthetic Peptide within Human GPR55 aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9Y2T6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GPR55 receptor also known as G protein-coupled receptor 55 or GPR-55 is a member of the G-protein-coupled receptor (GPCR) family. GPR55 is involved mechanically in cell signaling by interacting with ligands to activate intracellular signal transduction pathways. It has a molecular mass of approximately 37 kDa. This receptor is widely distributed in tissues including the brain spleen and gastrointestinal tract which supports its involvement in various physiological processes.

Biological function summary

GPR55 influences numerous functions such as modulation of neurotransmitter release and immune responses. Although not part of a larger receptor complex it acts independently as a signaling entity. GPR55 is involved in the regulation of blood pressure potentially influencing cardiovascular health. Its expression and activity in the central nervous system suggest roles in nervous system signaling and regulation.

Pathways

GPR55 integrates into the cannabinoid signaling pathway and can impact phosphatidylinositol-45-bisphosphate 3-kinase (PI3K) pathway. GPR55 interacts closely with other cannabinoid receptors like CB1 and CB2 although they functionally differ. This receptor's involvement provides flexibility in the signaling networks important for maintaining homeostasis and cellular responses to external stimuli.

GPR55 has associations with cancer and inflammation. Its signaling interference may contribute to tumor growth and could alter inflammatory responses. The receptor shares pathogenetic pathways with proteins such as CB1 and PI3K both of which influence oncogenic and inflammatory mechanisms. Understanding these interactions is essential for targeting GPR55 in therapeutic development for related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

G-protein coupled receptor that binds to several ligands including 2-arachidonoyl lysophosphatidylinositol or lysophosphatidylglucoside with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways (PubMed : 36142844, PubMed : 36523570, PubMed : 37544935). Induces the Ca(2+) release from intracellular stores via ERK, the heterotrimeric G protein GNA13 and RHOA leading to morphological changes including cell rounding and stress fiber formation (PubMed : 36142844). In macrophages, acts downstream of lysophosphatidylglucoside to inhibit the translocation of the phospholipid-transporting ABCA1 to plasma membrane and subsequent cholesterol efflux leading to lipid accumulation and foam cell formation (PubMed : 37544935).

See full target information GPR55

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

ACS omega 9:21838-21850 PubMed38799363

2024

Activation of GPR55 Ameliorates Maternal Separation-Induced Learning and Memory Deficits by Augmenting 5-HT Synthesis in the Dorsal Raphe Nucleus of Juvenile Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Sun,Ya-Ya Du,Yong-Qiang Zhang,Qin-Qin Tian,Xi Li,Jiao-Yan Yu,Yan-Yan Guo,Qing-Qing Liu,Le Yang,Yu-Mei Wu,Qi Yang,Ming-Gao Zhao

Heliyon 10:e30462 PubMed38720745

2024

GPR55 activation improves anxiety- and depression-like behaviors of mice during methamphetamine withdrawal.

Applications

Unspecified application

Species

Unspecified reactive species

Jinlong Zhang,Jie Yan,Shuyue Li,Qianqian Chen,Jiang Lin,Yilin Peng,Yuhang Liu,Binbin Wang,Xinrong Wei,Chen Sun,Shuliang Niu

PloS one 18:e0280842 PubMed36701411

2023

Heterozygous deletion of Gpr55 does not affect a hyperthermia-induced seizure, spontaneous seizures or survival in the Scn1a+/- mouse model of Dravet syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Lyndsey L Anderson,Dilara A Bahceci,Nicole A Hawkins,Declan Everett-Morgan,Samuel D Banister,Jennifer A Kearney,Jonathon C Arnold

Journal of molecular neuroscience : MN 72:1859-1874 PubMed35779192

2022

Anti-Inflammatory Properties of KLS-13019: a Novel GPR55 Antagonist for Dorsal Root Ganglion and Hippocampal Cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Douglas E Brenneman,William A Kinney,Mark E McDonnell,Pingei Zhao,Mary E Abood,Sara Jane Ward

Molecular medicine reports 25: PubMed35244189

2022

CID16020046, a GPR55 antagonist, attenuates sepsis‑induced acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Rongxin Chen,Hailin Xu,Zebin Guo,Peng Zhang,Jianxia Chen,Zheng Chen

Pharmaceuticals (Basel, Switzerland) 15: PubMed35056124

2022

GPR55 and GPR119 Receptors Contribute to the Processing of Neuropathic Pain in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ángel Zúñiga-Romero,Quetzali Rivera-Plata,Jesús Arrieta,Francisco Javier Flores-Murrieta,Juan Rodríguez-Silverio,Juan Gerardo Reyes-García,Juan Carlos Huerta-Cruz,Gustavo Ramírez-Martínez,Héctor Isaac Rocha-González

International journal of molecular sciences 22: PubMed33435517

2021

GPR55 Receptor Activation by the -Acyl Dopamine Family Lipids Induces Apoptosis in Cancer Cells via the Nitric Oxide Synthase (nNOS) Over-Stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Mikhail G Akimov,Alina M Gamisonia,Polina V Dudina,Natalia M Gretskaya,Anastasia A Gaydaryova,Andrey S Kuznetsov,Galina N Zinchenko,Vladimir V Bezuglov

International journal of molecular sciences 21: PubMed32075117

2020

Chronic Cannabidiol Administration Fails to Diminish Blood Pressure in Rats with Primary and Secondary Hypertension Despite Its Effects on Cardiac and Plasma Endocannabinoid System, Oxidative Stress and Lipid Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Patryk Remiszewski,Iwona Jarocka-Karpowicz,Michał Biernacki,Anna Jastrząb,Eberhard Schlicker,Marek Toczek,Ewa Harasim-Symbor,Anna Pędzińska-Betiuk,Barbara Malinowska

Molecular brain 10:38 PubMed28800762

2017

The novel cannabinoid receptor GPR55 mediates anxiolytic-like effects in the medial orbital cortex of mice with acute stress.

Applications

WB, IHC-FoFr

Species

Mouse, Mouse

Qi-Xin Shi,Liu-Kun Yang,Wen-Long Shi,Lu Wang,Shi-Meng Zhou,Shao-Yu Guan,Ming-Gao Zhao,Qi Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-GPCR GPR55 antibody

Western blot - Anti-GPCR GPR55 antibody (AB203663)

Western blot - Anti-GPCR GPR55 antibody (AB203663)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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