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AB272390

Anti-GPCR GPR64 antibody [EPR23658-2]

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Rabbit Recombinant Monoclonal GPCR GPR64 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.

View Alternative Names

GPR64, HE6, TM7LN2, ADGRG2, Adhesion G-protein coupled receptor G2, G-protein coupled receptor 64, Human epididymis-specific protein 6, He6

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] (AB272390)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] (AB272390)

Immunohistochemical analysis of paraffin-embedded Human Ewing sarcoma tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human Ewing sarcoma (PMID : 23338946). The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] (AB272390)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] (AB272390)

Immunohistochemical analysis of paraffin-embedded Rat epididymis tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in rat epididymis (PMID : 27476656, 12420295, 18469038).The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] (AB272390)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] (AB272390)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : No staining in rat cerebrum(PMID : 12420295).The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] (AB272390)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] (AB272390)

Immunohistochemical analysis of paraffin-embedded Mouse epididymis tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse epididymis (PMID : 27476656, 12420295, 18469038).The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] (AB272390)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] (AB272390)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : No staining in mouse liver (PMID : 12420295).The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Western blot - Anti-GPCR GPR64 antibody [EPR23658-2] (AB272390)
  • WB

Lab

Western blot - Anti-GPCR GPR64 antibody [EPR23658-2] (AB272390)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The observed molecular weight is consistent with what has been described in the literature (PMID : 12420295, 29393851).

Two bands of 180 and 130 KDa in lane 1 are glycosylated forms. The bands around 105KDa in lane 2 are de-glycosylated and partially de-glycosylated forms.

Exposure time : 54 seconds

All lanes:

Western blot - Anti-GPCR GPR64 antibody [EPR23658-2] (ab272390) at 1/1000 dilution

Lane 1:

A673 (human muscle Ewing's Sarcoma), whole cell lysate at 20 µg

Lane 2:

A673 whole cell lysate treated with PNGase F at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 112 kDa

Observed band size: 105 kDa,130 kDa,180 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] (AB272390)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] (AB272390)

Immunohistochemical analysis of paraffin-embedded Human leiomyosarcoma tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : No staining in human leiomyosarcoma (PMID : 23338946).The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR64 antibody [EPR23658-2] (AB272390)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR64 antibody [EPR23658-2] (AB272390)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A-673 cells labelling GPCR GPR64 with ab272390 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and membranous staining in A-673 cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.

  • Carrier free

    Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23658-2

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GPCR GPR64 also known as ADGRG2 is a member of the adhesion G protein-coupled receptor family. It is characterized by large extracellular domains facilitating cell adhesion and signaling. This receptor has a mass of approximately 98 kDa. GPR64 is mainly expressed in the epididymis and plays an important role in reproductive tissues. Moreover it displays a basal activity that implicates several signaling pathways.
Biological function summary

GPCR GPR64 functions as a mediator of reproductive tract development. It is not currently known to be part of any larger complex. However its signaling contributes to the modulation of fluid reabsorption in the epididymis affecting sperm maturation and storage. Beyond the reproductive system some studies suggest roles in other tissues like osteoblasts and kidney cells.

Pathways

Several pieces of research have tied GPCR GPR64 to the cAMP signaling pathway influencing the regulation of epithelial sodium channels. This impacts ion transport and homeostasis within the reproductive tissues. Additionally other proteins such as epithelial sodium channel (ENaC) have shown interactions with GPR64 affecting fluid dynamics. Further research is exploring potential connections to the Wnt signaling pathway due to similar functional roles.

GPCR GPR64 has been linked to disorders affecting fertility such as reduced sperm motility leading to male infertility. Also abnormal expression patterns have been observed in some cancer types like prostate cancer. The interaction with ENaC makes it particularly interesting in understanding how dysregulation might impact these conditions on a cellular level providing insights for potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Orphan receptor. Could be involved in a signal transduction pathway controlling epididymal function and male fertility. May regulate fluid exchange within epididymis.
See full target information ADGRG2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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