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AB272395

Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free

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Rabbit Recombinant Monoclonal GPCR GPR64 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse samples.
7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (AB272395)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (AB272395)

Immunohistochemical analysis of paraffin-embedded Human Ewing sarcoma tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human Ewing sarcoma (PMID : 23338946). The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272390).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (AB272395)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (AB272395)

Immunohistochemical analysis of paraffin-embedded Human leiomyosarcoma tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : No staining in human leiomyosarcoma (PMID : 23338946).The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272390).

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (AB272395)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (AB272395)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A-673 cells labelling GPCR GPR64 with ab272390 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and membranous staining in A-673 cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272390).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (AB272395)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (AB272395)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : No staining in rat cerebrum(PMID : 12420295).The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272390).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (AB272395)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (AB272395)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : No staining in mouse liver (PMID : 12420295).The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272390).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (AB272395)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (AB272395)

Immunohistochemical analysis of paraffin-embedded Rat epididymis tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in rat epididymis (PMID : 27476656, 12420295, 18469038).The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272390).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (AB272395)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (AB272395)

Immunohistochemical analysis of paraffin-embedded Mouse epididymis tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse epididymis (PMID : 27476656, 12420295, 18469038).The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272390).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23658-2

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab272395 is the carrier-free version of ab272390.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GPCR GPR64 also known as ADGRG2 is a member of the adhesion G protein-coupled receptor family. It is characterized by large extracellular domains facilitating cell adhesion and signaling. This receptor has a mass of approximately 98 kDa. GPR64 is mainly expressed in the epididymis and plays an important role in reproductive tissues. Moreover it displays a basal activity that implicates several signaling pathways.
Biological function summary

GPCR GPR64 functions as a mediator of reproductive tract development. It is not currently known to be part of any larger complex. However its signaling contributes to the modulation of fluid reabsorption in the epididymis affecting sperm maturation and storage. Beyond the reproductive system some studies suggest roles in other tissues like osteoblasts and kidney cells.

Pathways

Several pieces of research have tied GPCR GPR64 to the cAMP signaling pathway influencing the regulation of epithelial sodium channels. This impacts ion transport and homeostasis within the reproductive tissues. Additionally other proteins such as epithelial sodium channel (ENaC) have shown interactions with GPR64 affecting fluid dynamics. Further research is exploring potential connections to the Wnt signaling pathway due to similar functional roles.

GPCR GPR64 has been linked to disorders affecting fertility such as reduced sperm motility leading to male infertility. Also abnormal expression patterns have been observed in some cancer types like prostate cancer. The interaction with ENaC makes it particularly interesting in understanding how dysregulation might impact these conditions on a cellular level providing insights for potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adhesion G-protein coupled receptor (aGPCR) for steroid hormones, such as dehydroepiandrosterone (DHEA; also named 3beta-hydroxyandrost-5-en-17-one) and androstenedione (PubMed : 29393851, PubMed : 35982227, PubMed : 39884271). Involved in a signal transduction pathway controlling epididymal function and male fertility (PubMed : 29393851). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed : 33303626, PubMed : 34234254). ADGRG2 is coupled to G(s) G proteins and mediates activation of adenylate cyclase activity (PubMed : 29393851, PubMed : 34234254). Also able to couple with G(q) G proteins in vitro (PubMed : 29393851). Together with CFTR, required to promote fluid reabsorption within efferent ductule (PubMed : 29393851).
See full target information ADGRG2

Alternative Names

GPR64, HE6, TM7LN2, ADGRG2, Adhesion G-protein coupled receptor G2, G-protein coupled receptor 64, Human epididymis-specific protein 6, He6
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com