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AB188907

Anti-GPCR GPR65/TDAG8 antibody

4

(3 Reviews)

|

(5 Publications)

Rabbit Polyclonal GPCR GPR65/TDAG8 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human GPR65.

View Alternative Names

TDAG8, GPR65, G-protein coupled receptor 65, Psychosine receptor, T-cell death-associated gene 8 protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR65/TDAG8 antibody (AB188907)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR65/TDAG8 antibody (AB188907)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for GPCR GPR65/TDAG8 with ab188907 at 10 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR65/TDAG8 antibody (AB188907)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR65/TDAG8 antibody (AB188907)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for GPCR GPR65/TDAG8 with ab188907 at 10 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR65/TDAG8 antibody (AB188907)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR65/TDAG8 antibody (AB188907)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for GPCR GPR65/TDAG8 with ab188907 at 10 μg/ml in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human GPR65. The exact immunogen used to generate this antibody is proprietary information.

Q8IYL9

Specificity

BLAST analysis of the peptide immunogen showed no homology with other human proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Bat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPR65 also known as TDAG8 is a G-protein-coupled receptor with an approximate mass of 37.8 kDa. This receptor is involved in sensing extracellular pH changes. It predominates in expression within immune cells including peripheral blood mononuclear cells (PBMCs) and is abundant in tissues like the lung and spleen. The receptor primarily localizes to the plasma membrane where it plays a role in cell signaling pathways by coupling with G-proteins.
Biological function summary

GPR65 contributes to the regulation of immune response and is implicated in the physiological process of proton-sensing. It is a part of signaling complexes that mediate the cellular response to acidic environments influencing processes like inflammation. The receptor modulates the cAMP pathway responding to extracellular acidity and impacting downstream effects on immune cell behavior such as cytokine production and cell migration.

Pathways

GPR65 is associated with the cyclic AMP (cAMP) signaling pathway and the acid-sensing pathway. It interacts with other proteins such as adenylyl cyclase to modulate cAMP levels influencing immune responses and cellular adaptations to acidic environments. GPR65 connects to pathways involving inflammatory responses and shows synergies with pathways regulated by proteins like GPR4 and OGR1 which also function as proton-sensing receptors.

GPR65 is linked to inflammatory conditions and psychiatric disorders. Studies suggest that dysregulation of GPR65 activity can contribute to inflammatory bowel diseases through abnormal immune responses. The receptor may also play a role in mood disorders where its function and expression levels correlate with altered neurochemical environments. It interacts with immune-modulatory proteins including potentially TNF-alpha affecting inflammation and influencing pathological processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Proton-sensing G-protein coupled receptor activated by extracellular pH, which is required to monitor pH changes and generate adaptive reactions (PubMed : 15326175, PubMed : 15618224, PubMed : 20855608, PubMed : 33478938, PubMed : 37722051, PubMed : 39753132, PubMed : 40211064). Activated by an optimal pH of 7.4 (PubMed : 39753132). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed : 15326175, PubMed : 15618224, PubMed : 37722051, PubMed : 39753132). GPR65 is mainly coupled to G(s) G proteins and mediates activation of adenylate cyclase activity (PubMed : 15618224, PubMed : 37722051, PubMed : 39753132). May also act as a receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids (PubMed : 11309421, PubMed : 15326175). Plays a role in immune response by maintaining lysosome function and regulating T-cell metabolism (PubMed : 27287411). Acts as a regulator of inflammation by mediating pH-sensing of extracellular acidification which takes place in inflamed tissues : activation regulates endo-lysosomal function of immune cells and T-cell metabolism (By similarity). Constitutively active in endosomes and stimulates adenylate cyclase production from endosomes independently from extracellular pH changes (PubMed : 39753132).
See full target information GPR65
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 21:238 PubMed37723567

2023

GPR65 inhibits human trophoblast cell adhesion through upregulation of MYLK and downregulation of fibronectin via cAMP-ERK signaling in a low pH environment.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Mao,Ying Feng,Yayun Zheng,Yaqiu Gao,Linyu Zhang,Xinrui Sun,Yilun Wu,Xiaofeng Zhu,Fang Ma

PLoS pathogens 19:e1011495 PubMed37418488

2023

An acidic microenvironment in Tuberculosis increases extracellular matrix degradation by regulating macrophage inflammatory responses.

Applications

Unspecified application

Species

Unspecified reactive species

Ashley M Whittington,Frances S Turner,Friedrich Baark,Sam Templeman,Daniela E Kirwan,Candice Roufosse,Nitya Krishnan,Brian D Robertson,Deborah L W Chong,Joanna C Porter,Robert H Gilman,Jon S Friedland

Journal of clinical medicine 12: PubMed36902589

2023

Expression of pH-Sensitive GPCRs in Peritoneal Carcinomatosis of Colorectal Cancer-First Results.

Applications

Unspecified application

Species

Unspecified reactive species

Philipp von Breitenbuch,Bernadett Kurz,Susanne Wallner,Florian Zeman,Christoph Brochhausen,Hans-Jürgen Schlitt,Stephan Schreml

Scientific reports 10:15327 PubMed32948783

2020

Expression profiles of proton-sensing G-protein coupled receptors in common skin tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Wybke Klatt,Susanne Wallner,Christoph Brochhausen,Judith A Stolwijk,Stephan Schreml

Experimental dermatology 28:66-71 PubMed30339292

2018

Expression of proton-sensing G-protein-coupled receptors in selected skin tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Anaïs Nassios,Susanne Wallner,Sebastian Haferkamp,Christoph Klingelhöffer,Christoph Brochhausen,Stephan Schreml
View all publications
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Product promise

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