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AB106942

Anti-GPCR GPR81 antibody

0

(1 Review)

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(7 Publications)

Goat Polyclonal GPCR GPR81 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human HCAR1 aa 200-250.

View Alternative Names

GPR104, GPR81, HCA1, FKSG80, HCAR1, Hydroxycarboxylic acid receptor 1, G-protein coupled receptor 104, G-protein coupled receptor 81

1 Images
Western blot - Anti-GPCR GPR81 antibody (AB106942)
  • WB

Unknown

Western blot - Anti-GPCR GPR81 antibody (AB106942)

An additional band of unknown identity was also consistently observed at 55 kDa. This band was successfully blocked by incubation with the immunizing peptide.

All lanes:

Western blot - Anti-GPCR GPR81 antibody (ab106942) at 0.5 µg/mL

All lanes:

Human liver lysate at 35 µg

Predicted band size: 39 kDa

Observed band size: 40 kDa,55 kDa

true

Exposure time: 1hr

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human HCAR1 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

Q9BXC0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

G protein-coupled receptor 81 (GPR81) also known as Hydroxycarboxylic acid receptor 1 (HCAR1) plays an important role in lactate signaling. It has a molecular mass of approximately 40 kDa. GPR81 is expressed mainly in adipose tissue as well as in muscle and liver cells. The receptor responds to changes in lactate levels in these tissues and influences metabolic processes accordingly.
Biological function summary

GPR81 mediates lactate sensing and regulates lipolysis. It does not form part of a larger complex but functions on its own transmitting signals inside the cell upon lactate binding. This action reduces the breakdown of lipids by inhibiting the enzyme adenylate cyclase which in turn decreases intracellular cAMP levels. By limiting lipolysis GPR81 plays a role in energy balance and metabolic homeostasis.

Pathways

GPR81 integrates into energy regulation and metabolic pathways. It is significantly involved in the lactate and lipid metabolism pathway connecting with adenylate cyclase as a downstream effector. The inhibition of adenylate cyclase reduces cAMP synthesis affecting the Protein Kinase A (PKA) signaling pathway. This pathway links GPR81 to other receptors that modulate energy homeostasis such as GPR109A.

Research connects GPR81 to obesity and type 2 diabetes. By modulating lipolysis in adipose tissue GPR81 impacts fat storage and insulin sensitivity. The receptor's activity could also indirectly influence pathways involving insulin receptor substrate (IRS) proteins key players in glucose regulation. Understanding and targeting GPR81 may provide therapeutic advantages for managing these metabolic conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a receptor for L-lactate and mediates its anti-lipolytic effect through a G(i)-protein-mediated pathway.
See full target information HCAR1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell discovery 10:115 PubMed39557828

2024

Sodium oligomannate disrupts the adherence of Rib bacteria to gut epithelia to block SAA-triggered Th1 inflammation in 5XFAD transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyi Wang,Zuoquan Xie,Jie Yuan,Enjing Jin,Wen Lian,Shuaishuai Chang,Guangqiang Sun,Zhengnan Feng,Hui Xu,Chen Du,Xinying Yang,Aihua Xia,Ji Qiu,Qingli Zhang,Feifei Lin,Jia Liu,Liang Li,Xiaoguang Du,Zhongping Xiao,Zhou Yi,Zhiyu Luo,Changrong Ge,Rui Li,Mingyue Zheng,Yi Jiang,Tao Wang,Jing Zhang,Qihao Guo,Meiyu Geng

CNS neuroscience & therapeutics 30:e14465 PubMed37830163

2023

Genome-wide DNA methylation analysis identifies potent CpG signature for temzolomide response in non-G-CIMP glioblastomas with unmethylated MGMT promoter: MGMT-dependent roles of GPR81.

Applications

Unspecified application

Species

Unspecified reactive species

Bao-Bao Liang,Yu-Hong Wang,Jing-Jing Huang,Shuai Lin,Guo-Chao Mao,Zhang-Jian Zhou,Wan-Jun Yan,Chang-You Shan,Hui-Zi Wu,Amandine Etcheverry,Ya-Long He,Fang-Fang Liu,Hua-Feng Kang,An-An Yin,Shu-Qun Zhang

Cells 12: PubMed36980280

2023

Extracellular Lactic Acidosis of the Tumor Microenvironment Drives Adipocyte-to-Myofibroblast Transition Fueling the Generation of Cancer-Associated Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Andreucci,Bianca Saveria Fioretto,Irene Rosa,Marco Matucci-Cerinic,Alessio Biagioni,Eloisa Romano,Lido Calorini,Mirko Manetti

Gut microbes 14:2127456 PubMed36195972

2022

spp. and their metabolite lactate protect against acute pancreatitis via inhibition of pancreatic and systemic inflammatory responses.

Applications

Unspecified application

Species

Unspecified reactive species

Han Li,Jinyan Xie,Xiuliu Guo,Guilian Yang,Bin Cai,Jingtianyi Liu,Mengjia Yue,Yixin Tang,Gan Wang,Shuxian Chen,Jialin Guo,Xuchen Qi,Donghai Wang,Huijun Zheng,Wei Liu,Hong Yu,Chunfeng Wang,Shu Jeffrey Zhu,Feng Guo

Molecular medicine reports 26: PubMed35856408

2022

Adipose‑derived mesenchymal stem cell‑derived HCAR1 regulates immune response in the attenuation of sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Wang,Pengfei Xuan,Hongjun Tian,Xinyu Hao,Jingping Yang,Xiyuan Xu,Lixia Qiao

Molecular medicine reports 22:1727-1736 PubMed32582969

2020

Effects of GPR81 silencing combined with cisplatin stimulation on biological function in hypopharyngeal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaojing Jia,Ou Xu,Jianxing Wang,Jinhui Dong,Xiumin Ren,Xiaofang Jia,Chunguang Shan

Cell communication and signaling : CCS 13:36 PubMed26208712

2015

L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation.

Applications

WB

Species

Human

Waldemar Wagner,Wojciech M Ciszewski,Katarzyna D Kania
View all publications
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