AB77517

Anti-GPCR GPR87 antibody

Name: Anti-GPCR GPR87 antibody (ab77517) | Abcam
Brand: Abcam Limited
SKU: AB77517
Price: 535 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal GPCR GPR87 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human GPR87.

View Alternative Names

GPR95, FKSG78, GPR87, G-protein coupled receptor 87, G-protein coupled receptor 95

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR87 antibody (AB77517)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR87 antibody (AB77517)

Immunofluorescence analysis of GPCR GPR87 in HUVEC cells using ab77517, at 1/500 dilution, in the absence or presence of immunising peptide.

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Western blot - Anti-GPCR GPR87 antibody (AB77517)

All lanes:

Western blot - Anti-GPCR GPR87 antibody (ab77517) at 1/500 dilution

Lane 1:

Extracts from HUVEC cells at 5 µg

Lane 2:

Extracts from HT-29 cells at 5 µg

Lane 3:

Extracts from HUVEC cells at 5 µg with immunising peptide

Predicted band size: 41 kDa

Observed band size: 20 kDa,25 kDa,44 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ELISA, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human GPR87. The exact immunogen used to generate this antibody is proprietary information.

Q9BY21

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPCR GPR87 also known as G protein-coupled receptor 87 is a transmembrane receptor involved in various signaling processes. It has a molecular weight of approximately 42 kDa. GPR87 is expressed in various tissues with higher levels in certain cancers such as lung and bladder cancers. This receptor belongs to the large GPCR family which functions in transmitting signals from outside the cell to the inside leading to various cellular responses.

Biological function summary

In mediating cellular communication GPR87 plays a role in autocrine signaling mechanisms. It is not typically part of a larger complex but works closely with ligands and other signaling molecules to exert its effects. Studies indicate that GPR87 activation could promote cell proliferation and survival particularly in cancerous tissues suggesting a role in oncogenic pathways and cellular homeostasis.

Pathways

Multiple studies indicate that GPR87 participates in the p53 and Wnt signaling pathways. It might modulate the expression of target proteins such as p53 which is an important player in cell cycle regulation and apoptosis. GPR87 interacts with other proteins within these pathways potentially contributing to the regulatory loops that control cell fate and proliferation.

Researchers link GPR87 with lung and bladder cancer due to its overexpression in these tissues. It correlates with tumor aggression and poor prognosis indicating its role in cancer progression. The p53 protein a well-known tumor suppressor interacts with GPR87 adding complexity to the regulatory networks involved in these cancers. Understanding GPR87's contributions to these diseases may offer insights for developing targeted therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for lysophosphatidic acid (LPA). Necessary for p53/TP53-dependent survival in response to DNA damage.

See full target information GPR87

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Amino acids 52:1285-1298 PubMed32918616

2020

Taurine attenuates gossypol-induced apoptosis of C2C12 mouse myoblasts via the GPR87-AMPK/AKT signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Yu,Yang Wang,Ping Li,Meng Li,Xuejun Gao

Anticancer research 40:733-741 PubMed32014915

2020

Inhibition of Cell-surface Molecular GPR87 With GPR87-suppressing Adenoviral Vector Disturb Tumor Proliferation in Lung Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Kita,Tetsuhiko Go,Nariyasu Nakashima,Dage Liu,Yoshimasa Tokunaga,Xia Zhang,Takayuki Nakano,Kazuhito Nii,Sung Soo Chang,Hiroyasu Yokomise

Molecular therapy oncolytics 17:384-393 PubMed32405536

2020

G Protein-Coupled Receptor GPR87 Promotes the Expansion of PDA Stem Cells through Activating JAK2/STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Jianxin Jiang,Chao Yu,Xingjun Guo,Hao Zhang,She Tian,Kun Cai,Zhiwei He,Chengyi Sun

Journal of agricultural and food chemistry 67:1927-1936 PubMed30678459

2019

Taurine Promotes Milk Synthesis via the GPR87-PI3K-SETD1A Signaling in BMECs.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Yu,Yang Wang,Zhe Wang,Yanxu Liu,Yang Yu,Xuejun Gao

International journal of molecular sciences 16:24319-31 PubMed26473854

2015

G Protein-Coupled Receptor 87 (GPR87) Promotes Cell Proliferation in Human Bladder Cancer Cells.

Applications

Species

Unspecified reactive species

Xia Zhang,Dage Liu,Yushi Hayashida,Homare Okazoe,Takeshi Hashimoto,Nobufumi Ueda,Mikio Sugimoto,Yoshiyuki Kakehi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GPCR GPR87 antibody

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR87 antibody (AB77517)

Western blot - Anti-GPCR GPR87 antibody (AB77517)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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