Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (AB315809)

This data was developed using ab315808, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human GPCR GPRC5D expression vector containing a Myc tag. (B) HEK-293T transfected with empty vector containing a Myc tag. Tissue labeling GPCR GPRC5D with ab315808 at 1/500 (1.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T transfected with a human GPCR GPRC5D expression vector containing a Myc tag, no staining on (B) HEK-293T transfected with empty vector containing a Myc tag. The section was incubated with ab315808 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (AB315809)

This data was developed using ab315808, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GPCR GPRC5D with ab315808 at 1/500 (1.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human kidney. The section was incubated with ab315808 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (AB315809)

This data was developed using ab315808, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling GPCR GPRC5D with ab315808 at 1/500 (1.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human hepatocellular carcinoma. The section was incubated with ab315808 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (AB315809)

This data was developed using ab315808, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling GPCR GPRC5D with ab315808 at 1/500 (1.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human cerebrum. The section was incubated with ab315808 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (AB315809)

This data was developed using ab315808, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human myeloma tissue labeling GPCR GPRC5D with ab315808 at 1/500 (1.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human myeloma (PMID : 30918115). The section was incubated with ab315808 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (AB315809)

This data was developed using ab315808, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human hair follicle tissue labeling GPCR GPRC5D with ab315808 at 1/500 (1.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human hair follicle (PMID : 30918115). The section was incubated with ab315808 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (AB315809)

This data was developed using ab315808, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the higher MW band at approximately 50 kDa (in lane 2) is unknown.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

In Western blot, Anti-Integrin alpha V antibody [EPR16800] (ab179475) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] (<a href='/en-us/products/primary-antibodies/gpcr-gprc5d-antibody-epr28376-41-ab315808'>ab315808</a>) at 1/1000 dilution

Lane 1:

RPMI-8226 (human plasmacytoma, myeloma B lymphocyte) membrane fraction at 50 µg

Lane 2:

RPMI-8226 nuclear fraction at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 33 kDa,125 kDa,15 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (AB315809)

This data was developed using ab315808, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : human liver cancer.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] (<a href='/en-us/products/primary-antibodies/gpcr-gprc5d-antibody-epr28376-41-ab315808'>ab315808</a>) at 1/1000 dilution

Lane 1:

SK-MEL-28 (human malignant melanoma cell) whole cell lysate at 60 µg

Lane 2:

Human liver cancer tissue lysate at 60 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 33 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (AB315809)

This data was developed using ab315808, the same antibody clone in a different buffer formulation.

Negative control : Daudi (PMID : 32040549), KG-1a (PMID : 32040549).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the higher MW band at approximately 50 kDa is unknown.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] (<a href='/en-us/products/primary-antibodies/gpcr-gprc5d-antibody-epr28376-41-ab315808'>ab315808</a>) at 1/1000 dilution

Lane 1:

RPMI-8226 (human plasmacytoma, myeloma B lymphocyte) whole cell lysate at 50 µg

Lane 2:

Daudi (human burkitt's lymphoma lymphoblast) whole cell lysate at 50 µg

Lane 3:

SK-MEL-2 (human skin malignant melanoma cell) whole cell lysate at 50 µg

Lane 4:

KG-1a (human bone marrow myeloblast) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 33 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (AB315809)

This data was developed using ab315808, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

In Western blot, Anti-GFP antibody [E385] (ab32146) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] (<a href='/en-us/products/primary-antibodies/gpcr-gprc5d-antibody-epr28376-41-ab315808'>ab315808</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a GFP-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human GPCR GPRC5D expression vector containing a a GFP-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 33 kDa,15 kDa

false

Exposure time: 180s