Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (ab315809)

Rabbit Recombinant Monoclonal GPCR GPRC5D antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Transfected cell lysate - Human, Transfected cell line - Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (AB315809), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	IP	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Not recommended	Not recommended	Not recommended
Mouse	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Transfected cell line - Human	Not recommended	Tested	Not recommended	Not recommended	Not recommended
Transfected cell lysate - Human	Tested	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human, Transfected cell lysate - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Transfected cell line - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Transfected cell line - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Transfected cell lysate - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Transfected cell lysate - Human, Transfected cell line - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Transfected cell lysate - Human, Transfected cell line - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Transfected cell lysate - Human, Transfected cell line - Human	Dilution info -	Notes -

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

See full target information GPRC5D

Alternative names

G-protein coupled receptor family C group 5 member D, GPRC5D

Recommended products

Rabbit Recombinant Monoclonal GPCR GPRC5D antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Transfected cell lysate - Human, Transfected cell line - Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR28376-41
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab315809 is the carrier-free version of Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The GPCR GPRC5D also known as the GPRC5D protein or by the code 6D9 in certain research contexts is a member of the G-protein-coupled receptors (GPCRs) superfamily. This family acts as a significant mediator of cellular responses to external signals. The GPRC5D has a mass of approximately 56 kDa. It is mainly expressed in the human bone marrow and the keratinocytes of the skin with notable absence in other tissues. It also appears in certain immune cells indicating its potential involvement in immune system responses.

Biological function summary

The role of GPCR GPRC5D centers on cell signaling mechanisms within the immune system. GPCR GPRC5D engages in intracellular signaling cascades that modulate immune functions although it is not known to be part of a larger receptor complex. Its function may involve interactions with molecules that transmit environmental signals into cellular responses enhancing its importance in immune regulation and possibly other cellular functions.

Pathways

GPCR GPRC5D participates in signaling pathways like the immune regulation pathway and the skin development pathway. It relates to proteins such as arrestins which link GPCRs to downstream signaling pathways. This connection hints at a regulatory role in cellular response where GPCR GPRC5D acts as a conduit for signal transduction balancing cellular responses to external stimuli and maintaining cellular homeostasis.

Associated diseases and disorders

GPCR GPRC5D has associations with multiple myeloma and psoriasis. Its expression in bone marrow links it to multiple myeloma where its level might impact cancer progression. Proteins such as interleukin-17 and others relevant to psoriasis are connected to GPCR GPRC5D through skin-related pathways suggesting its role in inflammatory responses and potential as a therapeutic target for skin disorders. This illustrates GPRC5D's relevance in specific pathological conditions offering avenues for research into targeted treatments.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (ab315809)
This data was developed using Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human hair follicle tissue labeling GPCR GPRC5D with Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808 at 1/500 (1.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human hair follicle (PMID: 30918115). The section was incubated with Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (ab315809)
This data was developed using Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human myeloma tissue labeling GPCR GPRC5D with Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808 at 1/500 (1.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human myeloma (PMID: 30918115). The section was incubated with Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (ab315809)
This data was developed using Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808, the same antibody clone in a different buffer formulation.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.
In Western blot, Anti-GFP antibody [E385] (Anti-GFP antibody [E385] ab32146) staining at 1/5000 dilution.
All lanes: Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] (Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a GFP-tag, whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human GPCR GPRC5D expression vector containing a a GFP-tag, whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 33 kDa, 15 kDa
Exposure time: 180s
Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (ab315809)
This data was developed using Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808, the same antibody clone in a different buffer formulation.
Negative control: Daudi (PMID:32040549), KG-1a (PMID:32040549).
The identity of the higher MW band at approximately 50 kDa is unknown.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] (Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808) at 1/1000 dilution
Lane 1: RPMI-8226 (human plasmacytoma, myeloma B lymphocyte) whole cell lysate at 50 µg
Lane 2: Daudi (human burkitt's lymphoma lymphoblast) whole cell lysate at 50 µg
Lane 3: SK-MEL-2 (human skin malignant melanoma cell) whole cell lysate at 50 µg
Lane 4: KG-1a (human bone marrow myeloblast) whole cell lysate at 50 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 33 kDa, 36 kDa
Exposure time: 180s
Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (ab315809)
This data was developed using Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808, the same antibody clone in a different buffer formulation.
The identity of the higher MW band at approximately 50 kDa (in lane 2) is unknown.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.
In Western blot, Anti-Integrin alpha V antibody [EPR16800] (Anti-Integrin alpha V antibody [EPR16800] ab179475) staining at 1/5000 dilution.
All lanes: Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] (Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808) at 1/1000 dilution
Lane 1: RPMI-8226 (human plasmacytoma, myeloma B lymphocyte) membrane fraction at 50 µg
Lane 2: RPMI-8226 nuclear fraction at 50 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 33 kDa, 125 kDa, 15 kDa
Exposure time: 15s
Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (ab315809)
This data was developed using Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808, the same antibody clone in a different buffer formulation.
Negative control: human liver cancer.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] (Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808) at 1/1000 dilution
Lane 1: SK-MEL-28 (human malignant melanoma cell) whole cell lysate at 60 µg
Lane 2: Human liver cancer tissue lysate at 60 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 33 kDa, 36 kDa
Exposure time: 180s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (ab315809)
This data was developed using Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling GPCR GPRC5D with Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808 at 1/500 (1.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human hepatocellular carcinoma. The section was incubated with Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (ab315809)
This data was developed using Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GPCR GPRC5D with Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808 at 1/500 (1.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human kidney. The section was incubated with Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (ab315809)
This data was developed using Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human GPCR GPRC5D expression vector containing a Myc tag. (B) HEK-293T transfected with empty vector containing a Myc tag. Tissue labeling GPCR GPRC5D with Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808 at 1/500 (1.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) HEK-293T transfected with a human GPCR GPRC5D expression vector containing a Myc tag, no staining on (B) HEK-293T transfected with empty vector containing a Myc tag. The section was incubated with Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free (ab315809)
This data was developed using Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling GPCR GPRC5D with Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808 at 1/500 (1.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human cerebrum. The section was incubated with Anti-GPCR GPRC5D antibody [EPR28376-41] ab315808 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.