Anti-GPCR LGR6 antibody [EPR6874]

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-GPCR LGR6 antibody [EPR6874] (AB126747)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GPCR LGR6 with Purified ab126747 at 1/20 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488 ,ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR LGR6 antibody [EPR6874] (AB126747)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer tissue sections labeling GPCR LGR6 with purified ab126747 at 1/240 dilution (0.61 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-GPCR LGR6 antibody [EPR6874] (AB126747)

Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GPCR LGR6 with Purified ab126747 at 1 : 50 dilution (3.0 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR LGR6 antibody [EPR6874] (AB126747)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling GPCR LGR6 with purified ab126747 at 1/240 dilution (0.61 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR LGR6 antibody [EPR6874] (AB126747)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling GPCR LGR6 with purified ab126747 at 1/240 dilution (0.61 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• WB

Supplier Data

Western blot - Anti-GPCR LGR6 antibody [EPR6874] (AB126747)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody is specific to GPCR GPR48/LGR4 and does not cross react with endogenous GPCR LGR6.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 24205130, PMID : 11401528).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GPCR LGR6 antibody [EPR6874] (ab126747) - Total protein control staining at 1/1000 dilution.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

The high-sensitivity ECL substrate allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-GPCR GPR48/LGR4 antibody [EPR26351-175] (<a href='/en-us/products/primary-antibodies/gpcr-gpr48-lgr4-antibody-epr26351-175-ab321789'>ab321789</a>) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 40 µg

Lane 2:

A549 transfected with siRNA specifically targeting GPCR GPR48/LGR4 whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 110-120 kDa,36 kDa,104 kDa

true

Exposure time: 180s

• WB

Unknown

Western blot - Anti-GPCR LGR6 antibody [EPR6874] (AB126747)

We are not sure about the extra band.

All lanes:

Western blot - Anti-GPCR LGR6 antibody [EPR6874] (ab126747) at 1/1000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HUVEC (Human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 4:

SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysate at 20 µg

Lane 5:

Mouse liver lysate at 20 µg

Lane 6:

Rat liver lysate at 20 µg

Lane 7:

Human liver lysate at 20 µg

Lane 8:

Mouse lung lysate at 20 µg

Lane 9:

Rat lung lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 104 kDa

false

• WB

Lab

Western blot - Anti-GPCR LGR6 antibody [EPR6874] (AB126747)

Recombinant Human GPCR LGR6 protein (Cat# : ab153472)

All lanes:

Western blot - Anti-GPCR LGR6 antibody [EPR6874] (ab126747) at 1/1000 dilution

All lanes:

GST-Tagged Recombinant Human GPCR LGR6 protein at 0.015 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 104 kDa

false

Exposure time: 10s