AB72100

Anti-GPCR RDC1/CXCR-7 antibody

Name: Anti-GPCR RDC1/CXCR-7 antibody (ab72100) | Abcam
Brand: Abcam Limited
SKU: AB72100
Rating: 4 (1 reviews)

(1 Review)

(44 Publications)

Rabbit Polyclonal GPCR RDC1/CXCR-7 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 44 publications. Immunogen corresponding to Synthetic Peptide within Human ACKR3 aa 100-150.

View Alternative Names

CMKOR1, CXCR7, GPR159, RDC1, ACKR3, Atypical chemokine receptor 3, C-X-C chemokine receptor type 7, Chemokine orphan receptor 1, G-protein coupled receptor 159, G-protein coupled receptor RDC1 homolog, CXC-R7, CXCR-7, RDC-1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (AB72100)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (AB72100)

Immunohistochemical analysis of GPCR RDC1/CXCR-7 in paraffin-embedded formalin-fixed human tonsil tissue using ab72100 at 10 ug/ml

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (AB72100)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human tonsil staining GPCR RDC1/CXCR-7 with ab72100 (5ug/ml). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (AB72100)

IHC analysis of a formalin fixed paraffin embedded tissue section of human tonsil using 5ug/ml concentration ofab72100. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Supplier Data

Western blot - Anti-GPCR RDC1/CXCR-7 antibody (AB72100)

Blocking buffer : 5% non-fat milk in TBST.

All lanes:

Western blot - Anti-GPCR RDC1/CXCR-7 antibody (ab72100) at 2 µg/mL

Lane 1:

Human spleen

Lane 2:

Mouse spleen

Lane 3:

Human tonsil

Lane 4:

Human lymph node

Secondary

All lanes:

anti-rabbit HRP

Predicted band size: 41 kDa

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Unknown

Western blot - Anti-GPCR RDC1/CXCR-7 antibody (AB72100)

All lanes:

Western blot - Anti-GPCR RDC1/CXCR-7 antibody (ab72100) at 5 µg/mL

Lane 1:

Jurkat cell lysate

Lane 2:

Jurkat cell lysate with immunizing peptide

Predicted band size: 41 kDa

Observed band size: 29 kDa,42 kDa,65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ACKR3 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P25106

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "4-6 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "4-6 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPCR RDC1 also known as CXCR-7 is a G protein-coupled receptor that binds to chemokines such as CXCL12 and CXCL11. The receptor has a predicted molecular mass of approximately 40 kDa. It expresses widely in various tissues with high levels found in the heart liver kidney and brain. This receptor plays a role in the trafficking and localization of different cells often working alongside or complementing other chemokine receptors.

Biological function summary

GPCR RDC1/CXCR-7 takes part in complex cell signaling related to cell growth survival and migration. It is not usually part of a classic GPCR signaling pathway which sets it apart from other receptors in its family. Instead it can modulate pathways by forming heterodimers with other receptors such as CXCR4 affecting both ligand availability and receptor sensitivity. CXCR-7 can also function as a decoy receptor binding ligands without initiating a typical signaling cascade.

Pathways

The participation of GPCR RDC1/CXCR-7 is noted in chemokine-mediated pathways. Of particular importance is the SDF-1/CXCL12 signaling pathway which it shares with CXCR4. This pathway plays an important role in the regulation of stem cell migration and homing. CXCR-7 modulates the bioavailability of CXCL12 and influences how CXCR4 triggers downstream effects affecting cellular response to the external environment.

GPCR RDC1/CXCR-7 holds significance in cancer and cardiovascular diseases. In cancer overexpression of CXCR-7 associates with tumor growth and metastasis often in conjunction with the action of CXCR4. In cardiovascular conditions altered expression and functional shifts in CXCR-7 can lead to abnormal vascularization contributing to disease progression. Its role in these diseases makes CXCR-7 a valuable target for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CXCL11 and CXCL12/SDF1 (PubMed : 16107333, PubMed : 19255243, PubMed : 19380869, PubMed : 20161793, PubMed : 22300987). Chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway (PubMed : 16940167, PubMed : 18653785, PubMed : 20018651). Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness (PubMed : 16940167, PubMed : 18653785). In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival (PubMed : 16940167, PubMed : 20388803). Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration (PubMed : 17804806, PubMed : 18653785, PubMed : 19641136, PubMed : 20887389). Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12 (PubMed : 18653785). Required for heart valve development (PubMed : 17804806). Regulates axon guidance in the oculomotor system through the regulation of CXCL12 levels (PubMed : 31211835).. (Microbial infection) Acts as a coreceptor with CXCR4 for a restricted number of HIV isolates.

See full target information ACKR3

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Publications (44)

Recent publications for all applications. Explore the full list and refine your search

International immunopharmacology 144:113685 PubMed39608177

2024

The causative effect of CXCR7 on experimental autoimmune prostatitis injury and fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhang,Rui Feng,Sixu Chen,Zhengbin Wang,Cong Huang,Li Zhang,Jing Chen,Chaozhao Liang

Scientific reports 14:26985 PubMed39505939

2024

Chemokine receptor CXCR7 antagonism ameliorates cardiac and renal fibrosis induced by mineralocorticoid excess.

Applications

Unspecified application

Species

Unspecified reactive species

Bing H Wang,Remy Robert,Francine Z Marques,Niwanthi Rajapakse,Helen Kiriazis,Charles R Mackay,David M Kaye

European journal of clinical investigation 55:e14327 PubMed39373210

2024

ACKR3 agonism induces heterodimerization with chemokine receptor CXCR4 and attenuates platelet function.

Applications

Unspecified application

Species

Unspecified reactive species

Valerie Dicenta-Baunach,Zoi Laspa,David Schaale,Manuel Sigle,Alp Bayrak,Tatsiana Castor,Thanigaimalai Pillaiyar,Stefan Laufer,Meinrad Paul Gawaz,Anne-Katrin Rohlfing

Neurourology and urodynamics 43:2279-2289 PubMed39149821

2024

The SDF-1/CXCR4 axis is involved in adipose-derived stem cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang Li,Tibin Deng,Shaojie Zhu,Pingbo Xie,Wei Wang,Hongqing Zhou,Chenxiang Xu

Aging 15:1358-1370 PubMed36863713

2023

Ginkgolide A improves the pleiotropic function and reinforces the neuroprotective effects by mesenchymal stem cell-derived exosomes in 6-OHDA-induced cell model of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

William Shao-Tsu Chen,Tzu-Ying Lin,Chia-Hua Kuo,Dennis Jine-Yuan Hsieh,Wei-Wen Kuo,Shih-Chieh Liao,Hui-Chuan Kao,Da-Tong Ju,Yu-Jung Lin,Chih-Yang Huang

Arthritis research & therapy 24:261 PubMed36443856

2022

Epigenetic modification of miR-217 promotes intervertebral disc degeneration by targeting the FBXO21-ERK signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhonghui Chen,Jianghua Ming,Yajing Liu,Geliang Hu,Qi Liao

Cell & bioscience 12:171 PubMed36210463

2022

CXCL12/CXCR7/β-arrestin1 biased signal promotes epithelial-to-mesenchymal transition of colorectal cancer by repressing miRNAs through YAP1 nuclear translocation.

Applications

Unspecified application

Species

Unspecified reactive species

Mahan Si,Yujia Song,Xiaohui Wang,Dong Wang,Xiaohui Liu,Xianjun Qu,Zhiyu Song,Xinfeng Yu

International journal of molecular sciences 23: PubMed36233057

2022

CXCR4, CXCR5 and CD44 May Be Involved in Homing of Lymphoma Cells into the Eye in a Patient Derived Xenograft Homing Mouse Model for Primary Vitreoretinal Lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Neele Babst,Lisa K Isbell,Felix Rommel,Aysegul Tura,Mahdy Ranjbar,Salvatore Grisanti,Cordula Tschuch,Julia Schueler,Soroush Doostkam,Peter C Reinacher,Justus Duyster,Vinodh Kakkassery,Nikolas von Bubnoff

Journal of assisted reproduction and genetics 39:1237-1247 PubMed35513746

2022

Whole-organ decellularization of the human uterus and in vivo application of the bio-scaffolds in animal models.

Applications

Unspecified application

Species

Unspecified reactive species

Seyedeh Sima Daryabari,Kiarad Fendereski,Fariba Ghorbani,Mehrshad Dehnavi,Yazdan Shafikhani,Ara Omranipour,Sam Zeraatian-Nejad Davani,Masoumeh Majidi Zolbin,Seyed Mohammad Tavangar,Abdol-Mohammad Kajbafzadeh

Cell death & disease 13:380 PubMed35443745

2022

Exosomal miR-146a-5p and miR-155-5p promote CXCL12/CXCR7-induced metastasis of colorectal cancer by crosstalk with cancer-associated fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Wang,Xiaohui Wang,Yujia Song,Mahan Si,Yuqi Sun,Xiaohui Liu,Shuxiang Cui,Xianjun Qu,Xinfeng Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GPCR RDC1/CXCR-7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (AB72100)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (AB72100)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (AB72100)

Western blot - Anti-GPCR RDC1/CXCR-7 antibody (AB72100)

Western blot - Anti-GPCR RDC1/CXCR-7 antibody (AB72100)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (44)

Product promise