Anti-GPCR RDC1/CXCR-7 antibody [EPR29064-191]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody [EPR29064-191] (AB324431)

Immunohistochemical analysis of paraffin-embedded (A) MCF7 (human breast adenocarcinoma epithelial cell) cell pellet (B) LNcap (human prostate carcinoma epithelial cell) cell pellet (C) T-47D (human ductal breast epithelial tumor epithelial cell) cell pellet (D) HT-29 (human colorectal adenocarcinoma epithelial cell) cell pellet tissue labeling GPCR RDC1/CXCR-7 with ab324431 at 1/1000 (0.511 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on A : MCF7 cell pellet and B : LNcap cell pellet, no staining on C : T-47D cell pellet and D : HT-29 cell pellet (PMID : 25168820, PMID : 20388803 ). The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody [EPR29064-191] (AB324431)

Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma tissue labeling GPCR RDC1/CXCR-7 with ab324431 at 1/1000 (0.511 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on blood vessels in human clear cell carcinoma. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody [EPR29064-191] (AB324431)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human CXCR7 expression vector containing a his tag. (B) HEK-293T transfected with empty vector containing a his tag. tissue labeling GPCR RDC1/CXCR-7 with ab324431 at 1/1000 (0.511 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T transfected with a human CXCR7 expression vector containing a his tag, no staining on (B) HEK-293T transfected with empty vector containing a his tag. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody [EPR29064-191] (AB324431)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling GPCR RDC1/CXCR-7 with ab324431 at 1/1000 (0.511 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on blood vessels in human spleen. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody [EPR29064-191] (AB324431)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling GPCR RDC1/CXCR-7 with ab324431 at 1/1000 (0.511 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on blood vessels in human colon carcinoma. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-GPCR RDC1/CXCR-7 antibody [EPR29064-191] (AB324431)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling GPCR RDC1/CXCR-7 with ab324431 at 1/500 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing mambrane and cytoplasmic staining in MCF7 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : T-47D (PMID : 25168820).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• Flow Cyt

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Flow Cytometry - Anti-GPCR RDC1/CXCR-7 antibody [EPR29064-191] (AB324431)

Flow cytometric analysis of T-47D (human ductal breast epithelial tumor epithelial cell, Left) / MCF7 (human breast adenocarcinoma epithelial cell, Right) cells labelling GPCR RDC1/CXCR-7 with ab324431 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Negative control : T-47D (PMID : 25168820).

• Flow Cyt

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Flow Cytometry - Anti-GPCR RDC1/CXCR-7 antibody [EPR29064-191] (AB324431)

Flow cytometric analysis of HT-29 (human colorectal adenocarcinoma epithelial cell, Left) / LNCap (human prostate carcinoma epithelial cell, Right) cells labelling GPCR RDC1/CXCR-7 with ab324431 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Negative control : HT-29 (PMID : 20388803).