Skip to main content

Rabbit Recombinant Monoclonal GPCR RDC1/CXCR-7 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Flow Cytometry - Anti-GPCR RDC1/CXCR-7 antibody [EPR29064-22] (AB322663), expandable thumbnail
  • Flow Cytometry - Anti-GPCR RDC1/CXCR-7 antibody [EPR29064-22] (AB322663), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-GPCR RDC1/CXCR-7 antibody [EPR29064-22] (AB322663), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICC/IFIHC-P
Human
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CXCL11 and CXCL12/SDF1 (PubMed:16107333, PubMed:19255243, PubMed:19380869, PubMed:20161793, PubMed:22300987). Chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway (PubMed:16940167, PubMed:18653785, PubMed:20018651). Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness (PubMed:16940167, PubMed:18653785). In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival (PubMed:16940167, PubMed:20388803). Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration (PubMed:17804806, PubMed:18653785, PubMed:19641136, PubMed:20887389). Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12 (PubMed:18653785). Required for heart valve development (PubMed:17804806). Regulates axon guidance in the oculomotor system through the regulation of CXCL12 levels (PubMed:31211835). (Microbial infection) Acts as a coreceptor with CXCR4 for a restricted number of HIV isolates.

Alternative names

CMKOR1, CXCR7, GPR159, RDC1, ACKR3, Atypical chemokine receptor 3, C-X-C chemokine receptor type 7, Chemokine orphan receptor 1, G-protein coupled receptor 159, G-protein coupled receptor RDC1 homolog, CXC-R7, CXCR-7, RDC-1

Rabbit Recombinant Monoclonal GPCR RDC1/CXCR-7 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29064-22
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry - Anti-GPCR RDC1/CXCR-7 antibody [EPR29064-22] (ab322663), expandable thumbnail

    Flow Cytometry - Anti-GPCR RDC1/CXCR-7 antibody [EPR29064-22] (ab322663)

    Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell) (Right) / T-47D (human ductal breast epithelial tumor epithelial cell) (Left) cells labelling GPCR RDC1/CXCR-7 with ab322663 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/5000 dilution was used as the secondary antibody.

    Gated on viable cells.

    Negative control: T-47D (PMID: 25168820).

  • Flow Cytometry - Anti-GPCR RDC1/CXCR-7 antibody [EPR29064-22] (ab322663), expandable thumbnail

    Flow Cytometry - Anti-GPCR RDC1/CXCR-7 antibody [EPR29064-22] (ab322663)

    Flow cytometric analysis of LNCap (human prostate carcinoma epithelial cell) (Right) / HT-29 (human colorectal adenocarcinoma epithelial cell) (Left) cells labelling GPCR RDC1/CXCR-7 with ab322663 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/5000 dilution was used as the secondary antibody.

    Gated on viable cells.

    Negative control: HT-29 (PMID: 20388803).

  • Immunocytochemistry/ Immunofluorescence - Anti-GPCR RDC1/CXCR-7 antibody [EPR29064-22] (ab322663), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GPCR RDC1/CXCR-7 antibody [EPR29064-22] (ab322663)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling GPCR RDC1/CXCR-7 with ab322663 at 1/50 (10.54 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in MCF7 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

    Negative control: T-47D (PMID: 25168820).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com