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AB138509

Anti-GPCR RDC1/CXCR-7 antibody [EPR9321]

4

(1 Review)

|

(13 Publications)

Rabbit Recombinant Monoclonal GPCR RDC1/CXCR-7 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 13 publications.

View Alternative Names

CMKOR1, CXCR7, GPR159, RDC1, ACKR3, Atypical chemokine receptor 3, C-X-C chemokine receptor type 7, Chemokine orphan receptor 1, G-protein coupled receptor 159, G-protein coupled receptor RDC1 homolog, CXC-R7, CXCR-7, RDC-1

3 Images
Western blot - Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (AB138509)
  • WB

Unknown

Western blot - Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (AB138509)

All lanes:

Western blot - Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (ab138509) at 1/1000 dilution

Lane 1:

THP-1 cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Lane 4:

C6 cell lysate at 10 µg

Lane 5:

Raw 264.7 cell lysate at 10 µg

Lane 6:

NIH3T3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

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Western blot - Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (AB138509)
  • WB

Unknown

Western blot - Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (AB138509)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (ab138509) at 1/10000 dilution

All lanes:

THP-1 (Human monocytic leukemia monocyte) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 41 kDa

false

Western blot - Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (AB138509)
  • WB

Unknown

Western blot - Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (AB138509)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (ab138509) at 1/10000 dilution

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg

Lane 2:

C6 (Rat glial tumor glial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9321

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPCR RDC1 also known as CXCR-7 is a G protein-coupled receptor that binds to chemokines such as CXCL12 and CXCL11. The receptor has a predicted molecular mass of approximately 40 kDa. It expresses widely in various tissues with high levels found in the heart liver kidney and brain. This receptor plays a role in the trafficking and localization of different cells often working alongside or complementing other chemokine receptors.
Biological function summary

GPCR RDC1/CXCR-7 takes part in complex cell signaling related to cell growth survival and migration. It is not usually part of a classic GPCR signaling pathway which sets it apart from other receptors in its family. Instead it can modulate pathways by forming heterodimers with other receptors such as CXCR4 affecting both ligand availability and receptor sensitivity. CXCR-7 can also function as a decoy receptor binding ligands without initiating a typical signaling cascade.

Pathways

The participation of GPCR RDC1/CXCR-7 is noted in chemokine-mediated pathways. Of particular importance is the SDF-1/CXCL12 signaling pathway which it shares with CXCR4. This pathway plays an important role in the regulation of stem cell migration and homing. CXCR-7 modulates the bioavailability of CXCL12 and influences how CXCR4 triggers downstream effects affecting cellular response to the external environment.

GPCR RDC1/CXCR-7 holds significance in cancer and cardiovascular diseases. In cancer overexpression of CXCR-7 associates with tumor growth and metastasis often in conjunction with the action of CXCR4. In cardiovascular conditions altered expression and functional shifts in CXCR-7 can lead to abnormal vascularization contributing to disease progression. Its role in these diseases makes CXCR-7 a valuable target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CXCL11 and CXCL12/SDF1 (PubMed : 16107333, PubMed : 19255243, PubMed : 19380869, PubMed : 20161793, PubMed : 22300987). Chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway (PubMed : 16940167, PubMed : 18653785, PubMed : 20018651). Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness (PubMed : 16940167, PubMed : 18653785). In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival (PubMed : 16940167, PubMed : 20388803). Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration (PubMed : 17804806, PubMed : 18653785, PubMed : 19641136, PubMed : 20887389). Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12 (PubMed : 18653785). Required for heart valve development (PubMed : 17804806). Regulates axon guidance in the oculomotor system through the regulation of CXCL12 levels (PubMed : 31211835).. (Microbial infection) Acts as a coreceptor with CXCR4 for a restricted number of HIV isolates.
See full target information ACKR3
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed38994928

2024

Roxadustat Attenuates Adverse Remodeling Following Myocardial Infarction in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Marc-Michael Zaruba,Simon Staggl,Santhosh Kumar Ghadge,Thomas Maurer,Jasmina Gavranovic-Novakovic,Vivek Jeyakumar,Patric Schönherr,Andreas Wimmer,Gerhard Pölzl,Axel Bauer,Moritz Messner

Translational pediatrics 12:1634-1645 PubMed37814711

2023

CXCR7 promotes the migration of fibroblasts derived from patients with acquired laryngotracheal stenosis by NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Mengrou Xu,Bin Hu,Jiarui Chen,Limin Zhao,Jing Wang,Xiaoyan Li

Bioengineered 13:7425-7438 PubMed35264069

2022

C-X-C motif chemokine ligand 12 (CXCL12)/C-X-C motif chemokine receptor 7(CXCR7) regulates epithelial-mesenchymal transition process and promotes the metastasis of esophageal cancer by activating signal transducer and activator of transcription 3 (STAT3) pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Guo,Chang-Yong Tong,Jian-Guang Shi,Xin-Jian Li

Frontiers in molecular biosciences 8:652443 PubMed34746227

2021

Enzalutamide-Resistant Progression of Castration-Resistant Prostate Cancer Is Driven via the JAK2/STAT1-Dependent Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Luo,Xiaobing Yang,Spyridon P Basourakos,Xuemei Zuo,Dechao Wei,Jiahui Zhao,Mingchuan Li,Qiankun Li,Tao Feng,Pengju Guo,Yongguang Jiang

Stem cell research & therapy 12:264 PubMed33941256

2021

Upregulating CXCR7 accelerates endothelial progenitor cell-mediated endothelial repair by activating Akt/Keap-1/Nrf2 signaling in diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyu Jiang,Ruiting Li,Chaoyang Xiu,Xu Ma,Hui Hu,Liming Wei,Yihan Tang,Mingyang Tao,Jungong Zhao

Marine drugs 19: PubMed33805447

2021

Combination of Fish Oil and Selenium Enhances Anticancer Efficacy and Targets Multiple Signaling Pathways in Anti-VEGF Agent Treated-TNBC Tumor-Bearing Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Hung Guo,Simon Hsia,Chieh-Han Chung,Yi-Chun Lin,Min-Yi Shih,Pei-Chung Chen,Guoo-Shyng W Hsu,Ciou-Ting Fan,Chia-Lin Peng

Respiratory research 21:287 PubMed33129326

2020

Overexpression of CXCR7 accelerates tumor growth and metastasis of lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Liu,Qian Cheng,Dong-Sheng Xu,Wen Wang,Zheng Fang,Dong-Dong Xue,Ya Zheng,Alex H Chang,Yan-Jun Lei

Inflammation research : official journal of the European Histamine Research Society ... [et al.] 69:167-178 PubMed31865399

2019

Modulation of CXC-motif chemokine receptor 7, but not 4, expression is related to migration of the human prostate cancer cell LNCaP: regulation by androgen and inflammatory stimuli.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Yu,Quynhchi Pham,Liangli Lucy Yu,Thomas T Y Wang

Experimental and therapeutic medicine 18:1091-1098 PubMed31316604

2019

Forkhead box P3 gene silencing inhibits the expression of chemokines and chemokine receptors associated with cell growth, migration, and apoptosis in hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Ou,Guang-Tao Zhang,Pei-Kai Tian,Jing-Sen Chen,Ze-Wei Lin,Yong Xie,Ai-Hong Wang,Xiao-Ping Liu,Ji-Kui Liu

Experimental cell research 364:175-183 PubMed29408206

2018

The 5T4 oncofetal glycoprotein does not act as a general organizer of the CXCL12 system in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Malte Puchert,Christian Koch,Jürgen Engele
View all publications
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