Anti-GPCR TGR5 antibody

• WB

Supplier Data

Western blot - Anti-GPCR TGR5 antibody (AB72608)

Lane 1:

Western blot - Anti-GPCR TGR5 antibody (ab72608)

Lanes 2 - 3:

Western blot - Anti-GPCR TGR5 antibody (ab72608) at 1/1000 dilution

Lane 1:

Pre-immune serum

Lane 2:

Human spleen lysate

Lane 3:

Mouse spleen lysate

Predicted band size: 35 kDa

Observed band size: 27 kDa,33 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR TGR5 antibody (AB72608)

IHC image of ab72608 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72608, at 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR TGR5 antibody (AB72608)

Formalin-fixed, paraffin-embedded mouse colon tissue stained for GPCR TGR5 with ab72608 at 1/200 dilution in immunohistochemical analysis. DAB staining. Counter stained with Hematoxylin.

• WB

CiteAb

Western blot - Anti-GPCR TGR5 antibody (AB72608)

Western Blotting using Anti-GPCR TGR5 antibody, ab72608. Publication image from Cheung-Flynn, J. et al., 2015, Nat Commun, 26197299. Legend direct from paper.

Signalling in the ileum and liver is altered after biliary diversion.Immunoblots of (a) ileum and (b) liver protein obtained from lean, DIO and GB-IL mice 8 weeks after surgery. Expression was normalized to GAPDH. *P<0.05, **P<0.01 by one-way analysis of variance with Dunn's post-test. $P<0.05, $$P<0.01, $$$P<0.001 by two-tailed Students t-test. Values shown are mean±s.e.m. N of 3–8 per group.

false

• WB

CiteAb

Western blot - Anti-GPCR TGR5 antibody (AB72608)

Western Blotting using Anti-GPCR TGR5 antibody, ab72608. Publication image from Cheung-Flynn, J. et al., 2015, Nat Commun, 26197299. Legend direct from paper.

Signalling in the ileum and liver is altered after biliary diversion.Immunoblots of (a) ileum and (b) liver protein obtained from lean, DIO and GB-IL mice 8 weeks after surgery. Expression was normalized to GAPDH. *P<0.05, **P<0.01 by one-way analysis of variance with Dunn's post-test. $P<0.05, $$P<0.01, $$$P<0.001 by two-tailed Students t-test. Values shown are mean±s.e.m. N of 3–8 per group.

false