Anti-GPCR TGR5 antibody
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(39 Publications)
Anti-GPCR TGR5 antibody (ab72608) is a rabbit polyclonal antibody detecting GPCR TGR5 in Western Blot, IHC-P. Suitable for Human, Mouse.
- Over 30 publications
- Trusted since 2008
View Alternative Names
TGR5, GPBAR1, G-protein coupled bile acid receptor 1, G-protein coupled receptor GPCR19, Membrane-type receptor for bile acids, hBG37, hGPCR19, M-BAR, BG37
- WB
Supplier Data
Western blot - Anti-GPCR TGR5 antibody (AB72608)
Lane 1:
Western blot - Anti-GPCR TGR5 antibody (ab72608)
Lanes 2 - 3:
Western blot - Anti-GPCR TGR5 antibody (ab72608) at 1/1000 dilution
Lane 1:
Pre-immune serum
Lane 2:
Human spleen lysate
Lane 3:
Mouse spleen lysate
Predicted band size: 35 kDa
Observed band size: 27 kDa,33 kDa
false
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR TGR5 antibody (AB72608)
IHC image of ab72608 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72608, at 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR TGR5 antibody (AB72608)
Formalin-fixed, paraffin-embedded mouse colon tissue stained for GPCR TGR5 with ab72608 at 1/200 dilution in immunohistochemical analysis. DAB staining. Counter stained with Hematoxylin.
Reactivity data
Product details
Anti-GPCR TGR5 antibody (ab72608) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse samples.
What is the molecular weight of GPCR TGR5?
Anti-GPCR TGR5 (ab72608) specifically detects a band for GPCR TGR5 (UniProt: Q8TDU6) at a molecular weight of 35kDa.
Trusted by the scientific community
Anti-GPCR TGR5 (ab72608) was first used in a scientific publication in 2008 and has been cited over 30 times in peer-reviewed journals.
Properties and storage information
Form
Purity
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GPCR TGR5 plays a role in energy metabolism and the immune response. It does not form part of a larger receptor complex but interacts with different proteins to mediate its effects. Upon activation by bile acids TGR5 stimulates the production of cyclic AMP (cAMP) and regulates the release of various cytokines influencing metabolic rate and promoting anti-inflammatory effects.
Pathways
GPCR TGR5 links to the cAMP signaling pathway and plays a role in the brown adipose tissue activation pathway. It is closely related to proteins like adenylate cyclase which it activates to increase cAMP levels and potentially interacts with nuclear receptors modifying gene expression linked to energy homeostasis and inflammation control.
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Target data
Publications (39)
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Cell death & disease 14:583 PubMed37658045
2023
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Journal of translational medicine 21:498 PubMed37491256
2023
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Frontiers in oncology 12:1113785 PubMed36755861
2023
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Cells 12: PubMed36831193
2023
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International journal of clinical and experimental pathology 15:354-363 PubMed36237638
2022
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Gut microbes 14:2120744 PubMed36067404
2022
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Frontiers in cellular and infection microbiology 12:956528 PubMed35967856
2022
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The Journal of clinical investigation 132: PubMed35316215
2022
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Experimental and therapeutic medicine 23:163 PubMed35069844
2022
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Evidence-based complementary and alternative medicine : eCAM 2021:6076293 PubMed34925531
2021
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com