AB188585

Anti-GPD2 antibody [EPR14259]

Name: Anti-GPD2 antibody [EPR14259] (ab188585) | Abcam
Brand: Abcam Limited
SKU: AB188585
Price: 325 USD
Availability: InStock
Rating: 4 (3 reviews)

RabMAb
Recombinant
What is this?

(3 Reviews)

(15 Publications)

Rabbit Recombinant Monoclonal GPD2 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 15 publications.

View Alternative Names

GPD-M, GPDH-M, mitohondrial glycerophosphate dehydrogenase gene, mtGPD, mGDH, GPD2

3 Images

Flow Cytometry (Intracellular) - Anti-GPD2 antibody [EPR14259] (AB188585)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GPD2 antibody [EPR14259] (AB188585)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling GPD2 with ab188585 at 1/310 dilution (red) compared to a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Supplier Data

Immunoprecipitation - Anti-GPD2 antibody [EPR14259] (AB188585)

Western blot aalysis of GPD2 in HeLa cell lysate immunoprecipitated using ab188585 at 1/50 dilution.

Secondary antibody : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-GPD2 antibody [EPR14259] (ab188585)

Predicted band size: 81 kDa

false

Supplier Data

Western blot - Anti-GPD2 antibody [EPR14259] (AB188585)

All lanes:

Western blot - Anti-GPD2 antibody [EPR14259] (ab188585) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

MCF-7 cell lysate at 20 µg

Lane 3:

NIH:OVCAR-3 cell lysate at 20 µg

Lane 4:

U87-MG cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 81 kDa

Observed band size: 74 kDa

false

Carrier free

Anti-GPD2 antibody [EPR14259] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14259

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/310", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycerol-3-phosphate dehydrogenase 2 (GPD2) also known as GPDM-m and mGPDH is an enzyme that belongs to the glycerol-3-phosphate dehydrogenase family. GPD2 weighs approximately 74 kDa and is expressed in several tissues including the liver kidney and muscle. It primarily localizes in the mitochondrial inner membrane. Mechanically GPD2 catalyzes the oxidation of glycerol-3-phosphate to dihydroxyacetone phosphate playing an important role in energy metabolism by participating in the glycerol phosphate shuttle. This shuttle facilitates the transfer of reducing equivalents across the mitochondrial membrane.

Biological function summary

GPD2 plays a role in cellular energy production by linking lipid metabolism to carbohydrate metabolism. It forms part of a complex system supporting oxidative phosphorylation by transferring electrons to the mitochondrial electron transport chain. The activity of GPD2 helps sustain the NAD+/NADH balance which is essential for maintaining cellular redox states. Through its function GPD2 contributes to metabolic flexibility allowing cells to adapt to changes in fuel availability.

Pathways

GPD2 is involved in important energy production pathways such as the glycerol phosphate shuttle and the oxidative phosphorylation pathway. These pathways are important for efficient ATP production in eukaryotic cells. GPD2 interacts with other metabolic proteins like cytosolic glycerol-3-phosphate dehydrogenase (GPD1) which works in tandem to facilitate the shuttle of reducing equivalents between cytosol and mitochondria. Such processes ensure continuous energy supply necessary for cellular functions especially under conditions where carbohydrates and lipids serve interchangeably as energy sources.

GPD2 associates with conditions such as obesity and type 2 diabetes where energy metabolism is often disrupted. Altered GPD2 activity can lead to impaired mitochondrial function contributing to the pathogenesis of these metabolic disorders. Furthermore GPD2 links to proteins implicated in these diseases such as insulin receptor substrates and oxidative stress markers which further influence glucose and lipid homeostasis. Understanding GPD2's interaction with these elements helps in uncovering potential therapeutic targets for such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-responsive mitochondrial glycerol-3-phosphate dehydrogenase which seems to be a key component of the pancreatic beta-cell glucose-sensing device.

See full target information GPD2

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e70195 PubMed39576231

2024

Rapid modulation of interscapular brown adipose tissue mitochondrial activity by ketosis induced by 1,3-butanediol administration to rats.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Venditti,Gabriella Pinto,Vincenzo Migliaccio,Giuliana Panico,Gianluca Fasciolo,Rita De Matteis,Lillà Lionetti,Pieter de Lange,Stefania Serpico,Gaetana Napolitano,Claudio Agnisola,Angela Amoresano,Assunta Lombardi

PloS one 19:e0310530 PubMed39509399

2024

Isoscopoletin inhibits hepatocellular carcinoma cell proliferation via regulating glycolysis-related proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyao Ruan,Chen Wang,Jiawen Gu,Zhihui Zhu

iScience 27:109796 PubMed38832016

2024

Mitochondrial glycerol 3-phosphate dehydrogenase deficiency exacerbates lipotoxic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Qu,Xiufei Liu,Jiaran Zhu,Niexia He,Qingshan He,Linlin Zhang,Yuren Wang,Xiaoli Gong,Xin Xiong,Jinbo Liu,Chuan Wang,Gangyi Yang,Qingwu Yang,Gang Luo,Zhiming Zhu,Yi Zheng,Hongting Zheng

Cell death & disease 14:784 PubMed38036520

2023

The mitochondrial NADH shuttle system is a targetable vulnerability for Group 3 medulloblastoma in a hypoxic microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

J Contenti,Y Guo,A Mazzu,M Irondelle,M Rouleau,C Lago,G Leva,L Tiberi,I Ben-Sahra,F Bost,N M Mazure

The Journal of clinical investigation 133: PubMed36821389

2023

Kidney glycolysis serves as a mammalian phosphate sensor that maintains phosphate homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Zhou,Petra Simic,Iris Y Zhou,Peter Caravan,Xavier Vela Parada,Donghai Wen,Onica L Washington,Maria Shvedova,Kerry A Pierce,Clary B Clish,Michael Mannstadt,Tatsuya Kobayashi,Marc N Wein,Harald Jüppner,Eugene P Rhee

Animals : an open access journal from MDPI 12: PubMed36496922

2022

Effect of miR-143-3p from Extracellular Vesicles of Porcine Uterine Luminal Fluid on Porcine Trophoblast Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Ding,Qun Hu,Jianyu Gan,Xupeng Zang,Ting Gu,Zhenfang Wu,Gengyuan Cai,Linjun Hong

Cell 185:4654-4673.e28 PubMed36334589

2022

Architecture of the outbred brown fat proteome defines regulators of metabolic physiology.

Applications

Unspecified application

Species

Unspecified reactive species

Haopeng Xiao,Luiz H M Bozi,Yizhi Sun,Christopher L Riley,Vivek M Philip,Mandy Chen,Jiaming Li,Tian Zhang,Evanna L Mills,Margo P Emont,Wenfei Sun,Anita Reddy,Ryan Garrity,Jiani Long,Tobias Becher,Laura Potano Vitas,Dina Laznik-Bogoslavski,Martha Ordonez,Xinyue Liu,Xiong Chen,Yun Wang,Weihai Liu,Nhien Tran,Yitong Liu,Yang Zhang,Aaron M Cypess,Andrew P White,Yuchen He,Rebecca Deng,Heiko Schöder,Joao A Paulo,Mark P Jedrychowski,Alexander S Banks,Yu-Hua Tseng,Paul Cohen,Linus T Tsai,Evan D Rosen,Samuel Klein,Maria Chondronikola,Fiona E McAllister,Nick Van Bruggen,Edward L Huttlin,Bruce M Spiegelman,Gary A Churchill,Steven P Gygi,Edward T Chouchani

Diabetes 70:1372-1387 PubMed33741719

2021

Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Qu,Xiaoli Gong,Xiufei Liu,Rui Zhang,Yuren Wang,Bangliang Huang,Linlin Zhang,Hongting Zheng,Yi Zheng

Oxidative medicine and cellular longevity 2020:8683404 PubMed32566106

2020

Improvement of Endothelial Dysfunction of Berberine in Atherosclerotic Mice and Mechanism Exploring through TMT-Based Proteomics.

Applications

Unspecified application

Species

Unspecified reactive species

Wangxiao Tan,Yu Wang,Kaiyue Wang,Siwei Wang,Jinghua Liu,Xiaoyan Qin,Yongna Dai,Xiaoying Wang,Xiumei Gao

International journal of molecular sciences 20: PubMed31618976

2019

The Potential of the FSP1cre- Mouse Model for Studying Juvenile NAFLD.

Applications

Unspecified application

Species

Unspecified reactive species

Jiapeng Chen,Yan Zhuang,Ming Keat Sng,Nguan Soon Tan,Walter Wahli

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GPD2 antibody [EPR14259]

Flow Cytometry (Intracellular) - Anti-GPD2 antibody [EPR14259] (AB188585)

Immunoprecipitation - Anti-GPD2 antibody [EPR14259] (AB188585)

Western blot - Anti-GPD2 antibody [EPR14259] (AB188585)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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